BMRB Entry 16847

Title:
Assignments for LC8 bound to a peptide corresponding to residues 233-249 of neuronal Nitric Oxide Synthase
Deposition date:
2010-04-07
Original release date:
2010-04-30
Authors:
Hall, Justin
Citation:

Citation: Hall, Justin. "Assignments for LC8 bound to a peptide corresponding to residues 233-249 of neuronal Nitric Oxide Synthase"  .

Assembly members:

Assembly members:
LC8, polymer, 89 residues, Formula weight is not available
nNOS, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15Da

Data sets:
Data typeCount
15N chemical shifts89
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC81
2nNOS2

Entities:

Entity 1, LC8 89 residues - Formula weight is not available

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Entity 2, nNOS 17 residues - Formula weight is not available

1   METLYSASPMETGLYILEGLNVALASPARG
2   ASPLEUASPGLYLYSSERHIS

Samples:

sample_1: LC8, [U-98% 15N], 1 mM; nNOS 2 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; sodium citrate 50 mM; sodium chloride 50 mM; sodium azide 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift calculation

NMR spectrometers:

  • Bruker AM 600 MHz

Related Database Links:

BMRB 15076 15077 15078 15953 17692
PDB
EMBL CDQ62741 CDQ75050
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975 AAA36376 AAA62405 AAB49040 AAB60654 AAI56400
REF NP_001135068 NP_001177683 NP_001188181 NP_001245530 NP_001245836 NP_000611 NP_001191147 XP_001083352 XP_003832698 XP_003907271
SP O96860 Q24117 P29475
TPG DAA34424
DBJ BAA03895 BAG57679
AlphaFold O96860 Q24117 P29475

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks