BMRB Entry 15953

Title:
H55K mutant of LC8 at pH 6.7 from Drosophila Melanogaster
Deposition date:
2008-09-15
Original release date:
2008-09-30
Authors:
Hall, Justin; Hall, Andrea; Pursifull, Nathan; Barbar, Elisar
Citation:

Citation: Hall, Justin; Hall, Andrea; Pursifull, Nathan; Barbar, Elisar. "Differences in Dynamic Structure of LC8 Monomer, Dimer and Dimer-Peptide Complexes."  Biochemistry 47, 11940-11952 (2008).
PubMed: 18942858

Assembly members:

Assembly members:
H55K_mutant_of_LC8, polymer, 89 residues, 10372 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15da

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts88
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer, chain 11
2monomer, chain 21

Entities:

Entity 1, monomer, chain 1 89 residues - 10372 Da.

H55K is an obligate monomer of LC8

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPLYSCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Samples:

sample_1: H55K mutant of LC8, [U-13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 5 mM; DSS 5 mM; D2O 10%; glycerol 3%; Maleic Acid 5 mM; Roche Protease Inhibitor 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.7; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

Burrow_Owl vbeta, In house - chemical shift assignment

NMR spectrometers:

  • Bruker Magnex 600 MHz

Related Database Links:

BMRB 15076 15077 15078 16847 17692
PDB
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF NP_001177683 NP_001245530 NP_001245836 NP_477408 NP_525075
SP O96860 Q24117
AlphaFold Q24117 O96860

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks