BMRB Entry 15953

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15953

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Title:
H55K mutant of LC8 at pH 6.7 from Drosophila Melanogaster
Deposition date:
2008-09-15
Original release date:
2008-09-30
Authors:
Hall, Justin; Hall, Andrea; Pursifull, Nathan; Barbar, Elisar
Citation:

Citation: Hall, Justin; Hall, Andrea; Pursifull, Nathan; Barbar, Elisar. "Differences in Dynamic Structure of LC8 Monomer, Dimer and Dimer-Peptide Complexes."  Biochemistry 47, 11940-11952 (2008).
PubMed: 18942858

Assembly members:

Assembly members:
H55K_mutant_of_LC8, polymer, 89 residues, 10372 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15da

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H55K_mutant_of_LC8: MSDRKAVIKNADMSEEMQQD AVDCATQALEKYNIEKDIAA YIKKEFDKKYNPTWKCIVGR NFGSYVTHETRHFIYFYLGQ VAILLFKSG

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts88
1H chemical shifts87

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer, chain 11
2monomer, chain 21

Entities:

Entity 1, monomer, chain 1 89 residues - 10372 Da.

H55K is an obligate monomer of LC8

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPLYSCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Related Database Links:

BMRB 15076 15077 15078 16847 17692
PDB 1RHW 2P2T 2PG1 3BRI 3BRL 3DVH 3DVP 3DVT 3E2B 3FM7 3GLW 4QH7 4QH8
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF NP_001177683 NP_001245530 NP_001245836 NP_477408 NP_525075
SP O96860 Q24117
AlphaFold O96860 Q24117

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks