BMRB Entry 15953

Name: H55K mutant of LC8 at pH 6.7 from Drosophila Melanogaster
Published: 2008-09-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15953

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

H55K mutant of LC8 at pH 6.7 from Drosophila Melanogaster

Deposition date:

2008-09-15

Original release date:

2008-09-30

Authors:

Hall, Justin; Hall, Andrea; Pursifull, Nathan; Barbar, Elisar

Citation:

Citation: Hall, Justin; Hall, Andrea; Pursifull, Nathan; Barbar, Elisar. "Differences in Dynamic Structure of LC8 Monomer, Dimer and Dimer-Peptide Complexes." Biochemistry 47, 11940-11952 (2008).
PubMed: 18942858

Assembly members:

Assembly members:
H55K_mutant_of_LC8, polymer, 89 residues, 10372 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15da

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H55K_mutant_of_LC8: MSDRKAVIKNADMSEEMQQD AVDCATQALEKYNIEKDIAA YIKKEFDKKYNPTWKCIVGR NFGSYVTHETRHFIYFYLGQ VAILLFKSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	172
15N chemical shifts	88
1H chemical shifts	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer, chain 1	1
2	monomer, chain 2	1

Entities:

Entity 1, monomer, chain 1 89 residues - 10372 Da.

H55K is an obligate monomer of LC8

1

MET

SER

ASP

ARG

LYS

ALA

VAL

ILE

LYS

ASN

2

ALA

ASP

MET

SER

GLU

MET

GLN

ASP

3

ALA

VAL

ASP

CYS

ALA

THR

GLN

ALA

LEU

GLU

4

LYS

TYR

ASN

ILE

GLU

LYS

ASP

ILE

ALA

5

TYR

ILE

LYS

GLU

PHE

ASP

LYS

TYR

6

ASN

PRO

THR

TRP

LYS

CYS

ILE

VAL

GLY

ARG

7

ASN

PHE

GLY

SER

TYR

VAL

THR

HIS

GLU

THR

8

ARG

HIS

PHE

ILE

TYR

PHE

TYR

LEU

GLY

GLN

9

VAL

ALA

ILE

LEU

PHE

LYS

SER

GLY

Samples:

sample_1: H55K mutant of LC8, [U-13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 5 mM; DSS 5 mM; D2O 10%; glycerol 3%; Maleic Acid 5 mM; Roche Protease Inhibitor 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.7; pressure: 1 atm; temperature: 303.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

Burrow_Owl vbeta, In house - chemical shift assignment

NMR spectrometers:

Bruker Magnex 600 MHz

BMRB	15076 15077 15078 16847 17692
PDB	1RHW 2P2T 2PG1 3BRI 3BRL 3DVH 3DVP 3DVT 3E2B 3FM7 3GLW 4QH7 4QH8
GB	AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF	NP_001177683 NP_001245530 NP_001245836 NP_477408 NP_525075
SP	O96860 Q24117
AlphaFold	O96860 Q24117