BMRB Entry 16820

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16820
MolProbity Validation Chart

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Title:
SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN S8E FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D
Deposition date:
2010-04-01
Original release date:
2012-08-03
Authors:
Liu, Gaohua; Rossi, Paolo; Wang, Dongyan; Nwosu, Chioma; OWENS, LEAH; Xiao, Rong; Liu, Jinfeng; BARAN, MICHAEL; SWAPNA, G.V.T; ACTON, THOMAS; Rost, BURKHARD; Montelione, Gaetano
Citation:

Citation: Liu, Gaohua; Rossi, Paolo; Montelione, Gaetano. "SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN S8E FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D"  .

Assembly members:

Assembly members:
S8E, polymer, 294 residues, 33088.738 Da.

Natural source:

Natural source:   Common Name: Methanobacterium thermoautotrophicum   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S8E: GDRRVRLIRTRGGNTKVRLA SDTRINVVDPETGKVEIAEI RNVVENTANPHFVRRNIITR GAVVETNLGNVRVTSRPGQD GVINGVLIRELEHHHHHHGD RRVRLIRTRGGNTKVRLASD TRINVVDPETGKVEIAEIRN VVENTANPHFVRRNIITRGA VVETNLGNVRVTSRPGQDGV INGVLIRELEHHHHHHGDRR VRLIRTRGGNTKVRLASDTR INVVDPETGKVEIAEIRNVV ENTANPHFVRRNIITRGAVV ETNLGNVRVTSRPGQDGVIN GVLIRELEHHHHHH

Data sets:
Data typeCount
residual dipolar couplings45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S8E1

Entities:

Entity 1, S8E 294 residues - 33088.738 Da.

1   GLYASPARGARGVALARGLEUILEARGTHR
2   ARGGLYGLYASNTHRLYSVALARGLEUALA
3   SERASPTHRARGILEASNVALVALASPPRO
4   GLUTHRGLYLYSVALGLUILEALAGLUILE
5   ARGASNVALVALGLUASNTHRALAASNPRO
6   HISPHEVALARGARGASNILEILETHRARG
7   GLYALAVALVALGLUTHRASNLEUGLYASN
8   VALARGVALTHRSERARGPROGLYGLNASP
9   GLYVALILEASNGLYVALLEUILEARGGLU
10   LEUGLUHISHISHISHISHISHISGLYASP
11   ARGARGVALARGLEUILEARGTHRARGGLY
12   GLYASNTHRLYSVALARGLEUALASERASP
13   THRARGILEASNVALVALASPPROGLUTHR
14   GLYLYSVALGLUILEALAGLUILEARGASN
15   VALVALGLUASNTHRALAASNPROHISPHE
16   VALARGARGASNILEILETHRARGGLYALA
17   VALVALGLUTHRASNLEUGLYASNVALARG
18   VALTHRSERARGPROGLYGLNASPGLYVAL
19   ILEASNGLYVALLEUILEARGGLULEUGLU
20   HISHISHISHISHISHISGLYASPARGARG
21   VALARGLEUILEARGTHRARGGLYGLYASN
22   THRLYSVALARGLEUALASERASPTHRARG
23   ILEASNVALVALASPPROGLUTHRGLYLYS
24   VALGLUILEALAGLUILEARGASNVALVAL
25   GLUASNTHRALAASNPROHISPHEVALARG
26   ARGASNILEILETHRARGGLYALAVALVAL
27   GLUTHRASNLEUGLYASNVALARGVALTHR
28   SERARGPROGLYGLNASPGLYVALILEASN
29   GLYVALLEUILEARGGLULEUGLUHISHIS
30   HISHISHISHIS

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.94 mM; H2O 95%; D2O 5%

sample_2: entity, [U-10% 13C; U-99% 15N], 1.0 mM; H2O 95%; D2O 5%

sample_3: entity, [U-100% 13C; U-100% 15N], 0.94 mM; D2O 100%

sample_4: entity, [U-10% 13C; U-99% 15N], 1.0 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQC_TROSYsample_4anisotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinement, structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - data analysis, peak picking, refinement

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

PSVS, Bhattacharya and Montelione - data analysis, refinement

NMR spectrometers:

  • Bruker Avance 800 MHz
  • GE Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16091