BMRB Entry 16091

Name: SOLUTION STRUCTURE OF 30S RIBOSOMAL PROTEIN S8E; FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D
Published: 2009-02-17

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PDB ID: 2kcp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16091
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF 30S RIBOSOMAL PROTEIN S8E; FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D

Deposition date:

2008-12-24

Original release date:

2009-02-17

Authors:

Liu, Gaohua; Rossi, Paolo; Wang, Dongyan; NWOSU, CHIOMA; OWENS, LEAH; Xiao, Rong; Liu, Jinfeng; BARAN, MICHAEL; SWAPNA, G.V.T; ACTON, THOMAS; Rost, BURKHARD; MONTELIONE, GAETANO

Citation:

Citation: Liu, Gaohua; MONTELIONE, Gaetano. "SOLUTION STRUCTURE OF 30S RIBOSOMAL PROTEIN S8E FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET" .

Assembly members:

Assembly members:
rps8e, polymer, 294 residues, 33088.738 Da.

Natural source:

Natural source: Common Name: Methanobacterium thermoautotrophicum Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: not available Genus/species: Methanobacterium thermoautotrophicum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rps8e: GDRRVRLIRTRGGNTKVRLA SDTRINVVDPETGKVEIAEI RNVVENTANPHFVRRNIITR GAVVETNLGNVRVTSRPGQD GVINGVLIRELEHHHHHHGD RRVRLIRTRGGNTKVRLASD TRINVVDPETGKVEIAEIRN VVENTANPHFVRRNIITRGA VVETNLGNVRVTSRPGQDGV INGVLIRELEHHHHHHGDRR VRLIRTRGGNTKVRLASDTR INVVDPETGKVEIAEIRNVV ENTANPHFVRRNIITRGAVV ETNLGNVRVTSRPGQDGVIN GVLIRELEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	303
15N chemical shifts	97
1H chemical shifts	662

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rps8e	1

Entities:

Entity 1, rps8e 294 residues - 33088.738 Da.

1

GLY

ASP

ARG

VAL

ARG

LEU

ILE

ARG

THR

2

ARG

GLY

ASN

THR

LYS

VAL

ARG

LEU

ALA

3

SER

ASP

THR

ARG

ILE

ASN

VAL

ASP

PRO

4

GLU

THR

GLY

LYS

VAL

GLU

ILE

ALA

GLU

ILE

5

ARG

ASN

VAL

GLU

ASN

THR

ALA

ASN

PRO

6

HIS

PHE

VAL

ARG

ASN

ILE

THR

ARG

7

GLY

ALA

VAL

GLU

THR

ASN

LEU

GLY

ASN

8

VAL

ARG

VAL

THR

SER

ARG

PRO

GLY

GLN

ASP

9

GLY

VAL

ILE

ASN

GLY

VAL

LEU

ILE

ARG

GLU

10

LEU

GLU

HIS

GLY

ASP

11

ARG

VAL

ARG

LEU

ILE

ARG

THR

ARG

GLY

12

GLY

ASN

THR

LYS

VAL

ARG

LEU

ALA

SER

ASP

13

THR

ARG

ILE

ASN

VAL

ASP

PRO

GLU

THR

14

GLY

LYS

VAL

GLU

ILE

ALA

GLU

ILE

ARG

ASN

15

VAL

GLU

ASN

THR

ALA

ASN

PRO

HIS

PHE

16

VAL

ARG

ASN

ILE

THR

ARG

GLY

ALA

17

VAL

GLU

THR

ASN

LEU

GLY

ASN

VAL

ARG

18

VAL

THR

SER

ARG

PRO

GLY

GLN

ASP

GLY

VAL

19

ILE

ASN

GLY

VAL

LEU

ILE

ARG

GLU

LEU

GLU

20

HIS

GLY

ASP

ARG

21

VAL

ARG

LEU

ILE

ARG

THR

ARG

GLY

ASN

22

THR

LYS

VAL

ARG

LEU

ALA

SER

ASP

THR

ARG

23

ILE

ASN

VAL

ASP

PRO

GLU

THR

GLY

LYS

24

VAL

GLU

ILE

ALA

GLU

ILE

ARG

ASN

VAL

25

GLU

ASN

THR

ALA

ASN

PRO

HIS

PHE

VAL

ARG

26

ARG

ASN

ILE

THR

ARG

GLY

ALA

VAL

27

GLU

THR

ASN

LEU

GLY

ASN

VAL

ARG

VAL

THR

28

SER

ARG

PRO

GLY

GLN

ASP

GLY

VAL

ILE

ASN

29

GLY

VAL

LEU

ILE

ARG

GLU

LEU

GLU

HIS

30

HIS

Samples:

sample_1: rps8e, [U-100% 13C; U-100% 15N], 0.94 mM; H2O 95%; D2O 5%

sample_2: rps8e, [U-10% 13C; U-100% 15N], 1 mM; H2O 95%; D2O 5%

sample_1d20: rps8e, [U-100% 13C; U-100% 15N], 0.94 mM; D2O 100%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1d20	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1d20	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - geometry optimization, peak picking, refinement

PSVS, Bhattacharya and Montelione - geometry optimization

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 800 MHz
Varian INOVA 600 MHz