BMRB Entry 16741

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16741

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Title:
Backbone assignments for Paracoccus denitrificans amicyanin-CuI.
Deposition date:
2010-02-21
Original release date:
2010-10-05
Authors:
Ubbink, Marcellus
Citation:

Citation: Meschi, Francesca; Wiertz, Frank; Klauss, Linda; Cavalieri, Chiara; Blok, Anneloes; Ludwig, Bernd; Heering, Hendrik; Merli, Angelo; Rossi, Gian Luigi; Ubbink, Marcellus. "Amicyanin Transfers Electrons from Methylamine Dehydrogenase to Cytochrome c-551i via a Ping-Pong Mechanism, not a Ternary Complex."  J. Am. Chem. Soc. 132, 14537-14545 (2010).
PubMed: 20873742

Assembly members:

Assembly members:
amicyanin, polymer, 106 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Paracoccus denitrificans   Taxonomy ID: 266   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Paracoccus denitrificans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28ami

Entity Sequences (FASTA):

Entity Sequences (FASTA):
amicyanin: GDKATIPSESPFAAAEVADG AIVVDIAKMKYETPELHVKV GDTVTWINREAMPHNVHFVA GVLGEAALKGPMMKKEQAYS LTFTEAGTYDYHCTPHPFMR GKVVVE

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts97
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polypeptide1
2copper(I) ion2

Entities:

Entity 1, polypeptide 106 residues - Formula weight is not available

1   GLYASPLYSALATHRILEPROSERGLUSER
2   PROPHEALAALAALAGLUVALALAASPGLY
3   ALAILEVALVALASPILEALALYSMETLYS
4   TYRGLUTHRPROGLULEUHISVALLYSVAL
5   GLYASPTHRVALTHRTRPILEASNARGGLU
6   ALAMETPROHISASNVALHISPHEVALALA
7   GLYVALLEUGLYGLUALAALALEULYSGLY
8   PROMETMETLYSLYSGLUGLNALATYRSER
9   LEUTHRPHETHRGLUALAGLYTHRTYRASP
10   TYRHISCYSTHRPROHISPROPHEMETARG
11   GLYLYSVALVALVALGLU

Entity 2, copper(I) ion - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: amicyanin, [U-15N], 1.2 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

AZARA, Boucher - processing

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 16740
PDB
EMBL CAA39199
GB ABL72795
PRF 1702223A
REF WP_041530795
SP P22364
AlphaFold P22364

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks