BMRB Entry 16740

Name: Amide assignments for Paracoccus denitrificans amicyanin-ZnII.
Published: 2010-10-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16740

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Amide assignments for Paracoccus denitrificans amicyanin-ZnII.

Deposition date:

2010-02-21

Original release date:

2010-10-05

Authors:

Ubbink, Marcellus

Citation:

Citation: Meschi, Francesca; Wiertz, Frank; Klauss, Linda; Cavalieri, Chiara; Blok, Anneloes; Ludwig, Bernd; Heering, Hendrik; Merli, Angelo; Rossi, Gian Luigi; Ubbink, Marcellus. "Amicyanin Transfers Electrons from Methylamine Dehydrogenase to Cytochrome c-551i via a Ping-Pong Mechanism, not a Ternary Complex." J. Am. Chem. Soc. 132, 14537-14545 (2010).
PubMed: 20873742

Assembly members:

Assembly members:
amicyanin, polymer, 106 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Paracoccus denitrificans Taxonomy ID: 266 Superkingdom: Bacteria Kingdom: not available Genus/species: Paracoccus denitrificans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28ami

Entity Sequences (FASTA):

Entity Sequences (FASTA):
amicyanin: GDKATIPSESPFAAAEVADG AIVVDIAKMKYETPELHVKV GDTVTWINREAMPHNVHFVA GVLGEAALKGPMMKKEQAYS LTFTEAGTYDYHCTPHPFMR GKVVVE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	96
1H chemical shifts	98

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide	1
2	zinc(II) ion	2

Entities:

Entity 1, polypeptide 106 residues - Formula weight is not available

1

GLY

ASP

LYS

ALA

THR

ILE

PRO

SER

GLU

SER

2

PRO

PHE

ALA

GLU

VAL

ALA

ASP

GLY

3

ALA

ILE

VAL

ASP

ILE

ALA

LYS

MET

LYS

4

TYR

GLU

THR

PRO

GLU

LEU

HIS

VAL

LYS

VAL

5

GLY

ASP

THR

VAL

THR

TRP

ILE

ASN

ARG

GLU

6

ALA

MET

PRO

HIS

ASN

VAL

HIS

PHE

VAL

ALA

7

GLY

VAL

LEU

GLY

GLU

ALA

LEU

LYS

GLY

8

PRO

MET

LYS

GLU

GLN

ALA

TYR

SER

9

LEU

THR

PHE

THR

GLU

ALA

GLY

THR

TYR

ASP

10

TYR

HIS

CYS

THR

PRO

HIS

PRO

PHE

MET

ARG

11

GLY

LYS

VAL

GLU

Entity 2, zinc(II) ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: amicyanin, [U-15N], 1.2 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 7.9; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

AZARA, Boucher - processing

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

Bruker DMX 600 MHz

BMRB	16741
PDB	1AAC 1AAJ 1AAN 1BXA 1MDA 1MG2 1MG3 1SF3 1SF5 1SFD 1SFH 1T5K 2GB2 2GBA 2GC4 2GC7 2IDQ 2IDS 2IDT 2IDU 2J55 2J56 2J57 2MTA 2OV0 2QDV 2QDW 2RAC 3IE9 3IEA 3L45 3PLY 3RYM 4P5R 4P5S
EMBL	CAA39199
GB	ABL72795
PRF	1702223A
REF	WP_041530795
SP	P22364
AlphaFold	P22364