BMRB Entry 16621

Name: 1H, 13C, and 15N chemical shift assignment of KCNE3 in isotropic bicelles
Published: 2010-02-11

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PDB ID: 2ndj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16621
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignment of KCNE3 in isotropic bicelles

Deposition date:

2009-12-02

Original release date:

2010-02-11

Authors:

Kang, Congbao; Vanoye, Carlos; Welch, Richard; Sanders, Charles

Citation:

Citation: Kang, Congbao; Vanoye, Carlos; Welch, Richard; Van Horn, Wade; Sanders, Charles. "Functional delivery of a membrane protein into oocyte membranes using bicelles." Biochemistry 49, 653-655 (2010).
PubMed: 20044833

Assembly members:

Assembly members:
KCNE3, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KCNE3: MGHHHHHHGMETTNGTETWY ESLHAVLKALNATLHSNLLC RPGPGLGPDNQTERRASLPG RDDNSYMYILFVMFLFAVTV GSLILGYTRSRKVDKRSDPY HVYIKNRVSMI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	284
15N chemical shifts	96
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KCNE3	1

Entities:

Entity 1, KCNE3 111 residues - Formula weight is not available

1

MET

GLY

HIS

GLY

MET

2

GLU

THR

ASN

GLY

THR

GLU

THR

TRP

TYR

3

GLU

SER

LEU

HIS

ALA

VAL

LEU

LYS

ALA

LEU

4

ASN

ALA

THR

LEU

HIS

SER

ASN

LEU

CYS

5

ARG

PRO

GLY

PRO

GLY

LEU

GLY

PRO

ASP

ASN

6

GLN

THR

GLU

ARG

ALA

SER

LEU

PRO

GLY

7

ARG

ASP

ASN

SER

TYR

MET

TYR

ILE

LEU

8

PHE

VAL

MET

PHE

LEU

PHE

ALA

VAL

THR

VAL

9

GLY

SER

LEU

ILE

LEU

GLY

TYR

THR

ARG

SER

10

ARG

LYS

VAL

ASP

LYS

ARG

SER

ASP

PRO

TYR

11

HIS

VAL

TYR

ILE

LYS

ASN

ARG

VAL

SER

MET

12

ILE

Samples:

KCNE3_in_bicelles: KCNE3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; DTT 2 mM; EDTA 2 mM; bicelles 20%; D2O 10%; imidazole 250 mM; H2O 90%

sample_conditions_1: ionic strength: 0.250 M; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	KCNE3_in_bicelles	isotropic	sample_conditions_1
3D TROSY-HNCACB	KCNE3_in_bicelles	isotropic	sample_conditions_1
3D TROSY-CBCA(CO)NH	KCNE3_in_bicelles	isotropic	sample_conditions_1
3D TROSY-HNCA	KCNE3_in_bicelles	isotropic	sample_conditions_1
3D HN(CO)CA	KCNE3_in_bicelles	isotropic	sample_conditions_1
3D HNCO	KCNE3_in_bicelles	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - peak picking

NMR spectrometers:

Bruker Avance 800 MHz

GB	NM_005472 AAD28089 AAG16255 AAI10613 AAI13744 ABB00304
BMRB	16602
DBJ	BAG34817
EMBL	CAG33490 CAI56768
REF	NP_005463 XP_001115704 XP_001174917 XP_002822287 XP_003777893
SP	Q9Y6H6
AlphaFold	Q9Y6H6