BMRB Entry 16602

Name: 1H, 13C and 15N Chemical Shift Assignment Assignment of Human Membrane Protein KCNE3 in LMPC micelles
Published: 2010-02-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16602

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift Assignment Assignment of Human Membrane Protein KCNE3 in LMPC micelles

Deposition date:

2009-11-09

Original release date:

2010-02-11

Authors:

Kang, Congbao; Vanoye, Carlos; Sakakura, Masayoshi; Welch, Richard; Sanders, Charles

Citation:

Citation: Kang, Congbao; Vanoye, Carlos; Welch, Richard; Van Horn, Wade; Sanders, Charles. "Functional delivery of a membrane protein into oocyte membranes using bicelles." Biochemistry 49, 653-655 (2010).
PubMed: 20044833

Assembly members:

Assembly members:
KCNE3, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KCNE3: MGHHHHHHGMETTNGTETWY ESLHAVLKALNATLHSNLLC RPGPGLGPDNQTEERRASLP GRDDNSYMYILFVMFLFAVT VGSLILGYTRSRKVDKRSDP YHVYIKNRVSMI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	264
15N chemical shifts	91
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KCNE3	1

Entities:

Entity 1, KCNE3 112 residues - Formula weight is not available

The first nine residues are an affinity tag used in protein purification

1

MET

GLY

HIS

GLY

MET

2

GLU

THR

ASN

GLY

THR

GLU

THR

TRP

TYR

3

GLU

SER

LEU

HIS

ALA

VAL

LEU

LYS

ALA

LEU

4

ASN

ALA

THR

LEU

HIS

SER

ASN

LEU

CYS

5

ARG

PRO

GLY

PRO

GLY

LEU

GLY

PRO

ASP

ASN

6

GLN

THR

GLU

ARG

ALA

SER

LEU

PRO

7

GLY

ARG

ASP

ASN

SER

TYR

MET

TYR

ILE

8

LEU

PHE

VAL

MET

PHE

LEU

PHE

ALA

VAL

THR

9

VAL

GLY

SER

LEU

ILE

LEU

GLY

TYR

THR

ARG

10

SER

ARG

LYS

VAL

ASP

LYS

ARG

SER

ASP

PRO

11

TYR

HIS

VAL

TYR

ILE

LYS

ASN

ARG

VAL

SER

12

MET

ILE

Samples:

sample_KCNE3: D2O 10%; DTT 2 mM; EDTA 2 mM; imidazole 250 mM; LMPC 10%; KCNE3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM

sample_conditions_1: ionic strength: 0.25 M; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D TROSY-1H-15N HSQC	sample_KCNE3	isotropic	sample_conditions_1
3D TROSY-HNCA	sample_KCNE3	isotropic	sample_conditions_1
3D TROSY-CBCA(CO)NH	sample_KCNE3	isotropic	sample_conditions_1
3DTROSY- HN(CO)CA	sample_KCNE3	isotropic	sample_conditions_1
3D TROSYHNCACB	sample_KCNE3	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	16621
DBJ	BAG34817
EMBL	CAG33490 CAI56768
GB	AAD28089 AAG16255 AAI10613 AAI13744 ABB00304
REF	NP_005463 XP_001115704 XP_001174917 XP_002822287 XP_003777893
SP	Q9Y6H6
AlphaFold	Q9Y6H6