BMRB Entry 1658
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR1658
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Citation: Schmidt, Jurgen; Thuring, Harald; Werner, Angela; Ruterjans, Heinz; Quaas, Rainer; Hahn, Ulrich. "Two-dimensional 1H, 15N-NMR investigation of uniformly 15N-labeled ribonuclease
T1 (Complete assignment of 15N resonances)" Eur. J. Biochem. 197, 643-653 (1991).
Assembly members:
ribonuclease T1, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 5062 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Aspergillus oryzae
Experimental source: Production method: not available Host organism: Escherichia coli
Entity Sequences (FASTA):
ribonuclease T1: ACDYTCGSNCYSSSDVSTAQ
AAGYKLHEDGETVGSNSYPH
KYNNYEGFDFSVSSPYYEWP
ILSSGDVYSGGSPGADRVVF
NENNQLAGVITHTGASGNNF
VECT
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 120 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ribonuclease T1 | 1 |
Entities:
Entity 1, ribonuclease T1 104 residues - Formula weight is not available
| 1 | ALA | CYS | ASP | TYR | THR | CYS | GLY | SER | ASN | CYS | ||||
| 2 | TYR | SER | SER | SER | ASP | VAL | SER | THR | ALA | GLN | ||||
| 3 | ALA | ALA | GLY | TYR | LYS | LEU | HIS | GLU | ASP | GLY | ||||
| 4 | GLU | THR | VAL | GLY | SER | ASN | SER | TYR | PRO | HIS | ||||
| 5 | LYS | TYR | ASN | ASN | TYR | GLU | GLY | PHE | ASP | PHE | ||||
| 6 | SER | VAL | SER | SER | PRO | TYR | TYR | GLU | TRP | PRO | ||||
| 7 | ILE | LEU | SER | SER | GLY | ASP | VAL | TYR | SER | GLY | ||||
| 8 | GLY | SER | PRO | GLY | ALA | ASP | ARG | VAL | VAL | PHE | ||||
| 9 | ASN | GLU | ASN | ASN | GLN | LEU | ALA | GLY | VAL | ILE | ||||
| 10 | THR | HIS | THR | GLY | ALA | SER | GLY | ASN | ASN | PHE | ||||
| 11 | VAL | GLU | CYS | THR |
Samples:
sample_one:
sample_condition_set_one: pH: 5.5 na; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz