BMRB Entry 15905

Title:
1J coupling constants related to the Ca carbons in Ribonuclease T1 from Aspergillus oryzae
Deposition date:
2008-08-04
Original release date:
2008-11-19
Authors:
Schmidt, Jurgen; Lohr, Frank
Citation:

Citation: Schmidt, Jurgen; Howard, Mark; Maestre-Martinez, Mitcheell; Perez, Carlos; Lohr, Frank. "Variation in protein C(alpha)-related one-bond J couplings"  Magn. Reson. Chem. 47, 16-30 (2009).
PubMed: 18853398

Assembly members:

Assembly members:
RNase_T1, polymer, 104 residues, 11089.3 Da.

Natural source:

Natural source:   Common Name: Aspergillus oryzae   Taxonomy ID: 5062   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aspergillus oryzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pA2T1

Data sets:
Data typeCount
coupling constants360

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 104 residues - 11089.3 Da.

1   ALACYSASPTYRTHRCYSGLYSERASNCYS
2   TYRSERSERSERASPVALSERTHRALAGLN
3   ALAALAGLYTYRLYSLEUHISGLUASPGLY
4   GLUTHRVALGLYSERASNSERTYRPROHIS
5   LYSTYRASNASNTYRGLUGLYPHEASPPHE
6   SERVALSERSERPROTYRTYRGLUTRPPRO
7   ILELEUSERSERGLYASPVALTYRSERGLY
8   GLYSERPROGLYALAASPARGVALVALPHE
9   ASNGLUASNASNGLNLEUALAGLYVALILE
10   THRHISTHRGLYALASERGLYASNASNPHE
11   VALGLUCYSTHR

Samples:

doubly_labeled: RNase_T1, [U-95% 13C; U-95% 15N], 2 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D Ca-coupled [15N,1H]-TROSY-HNCOdoubly_labeledisotropicsample_conditions_1
2D IPAP-type HN(CO-a/b-NCa-J)-TROSYdoubly_labeledisotropicsample_conditions_1
3D Cb-coupled [15N,1H]-TROSY-HN(CO)CAdoubly_labeledisotropicsample_conditions_1
3D Ca-coupled HA(CACO)NHdoubly_labeledisotropicsample_conditions_1
3D Ha-coupled ct-[15N,1H]-TROSY-iHNCAdoubly_labeledisotropicsample_conditions_1
3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CAdoubly_labeledisotropicsample_conditions_1
3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlationdoubly_labeledisotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

jeval, JM Schmidt - coupling constant extraction, data analysis, multiplet simulation

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16469 1658 16580
PDB
DBJ BAA05707 BAA08407 BAE64671
EMBL CAA30560
GB AAA72541 AAA72994 EED52238 EIT74451 KDE77875
REF XP_001825804 XP_002377402
SP P00651
AlphaFold P00651