BMRB Entry 16575

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16575
MolProbity Validation Chart

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Title:
HUMAN NEDD4 3RD WW DOMAIN COMPLEX WITH EBOLA ZAIRE VIRUS MATRIX PROTEIN VP40 DERIVED PEPTIDE ILPTAPPEYMEA
Deposition date:
2009-10-23
Original release date:
2015-06-19
Authors:
Iglesias-Bexiga, Manuel; Luque, Irene; Macias, Maria; Blanco, Francisco; Cobos, Eva; Bonet, Roman
Citation:

Citation: Iglesias-Bexiga, Manuel. "HUMAN NEDD4 3RD WW DOMAIN COMPLEX WITH EBOLA ZAIRE VIRUS MATRIX PROTEIN VP40 DERIVED PEPTIDE"  .

Assembly members:

Assembly members:
NEDD4-WW3, polymer, 49 residues, 4213.847 Da.
ILPTAPPEYMEA, polymer, 12 residues, 1008.108 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NEDD4-WW3: GAMGPSEIEQGFLPKGWEVR HAPNGRPFFIDHNTKTTTWE DPRLKIPAH
ILPTAPPEYMEA: ILPTAPPEYMEA

Data sets:
Data typeCount
15N chemical shifts45
1H chemical shifts398

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NEDD4-WW31
2ILPTAPPEYMEA2

Entities:

Entity 1, NEDD4-WW3 49 residues - 4213.847 Da.

Residues 1-4 represent a non-native cloning tag resulting from TEV cleavage

1   GLYALAMETGLYPROSERGLUILEGLUGLN
2   GLYPHELEUPROLYSGLYTRPGLUVALARG
3   HISALAPROASNGLYARGPROPHEPHEILE
4   ASPHISASNTHRLYSTHRTHRTHRTRPGLU
5   ASPPROARGLEULYSILEPROALAHIS

Entity 2, ILPTAPPEYMEA 12 residues - 1008.108 Da.

1   ILELEUPROTHRALAPROPROGLUTYRMET
2   GLUALA

Samples:

15nSAMPLE: PROTEIN, [U-100% 15N], 1 mM; PEPTIDE 10 mM; sodium phosphate 20 mM; sodium azide 0.02 v/v; H2O 90%; D2O 10%

15N13Csample: PROTEIN, [U-100% 13C; U-100% 15N], 0.5 mM; PEPTIDE 5 mM; sodium phosphate 20 mM; sodium azide 0.02 v/v; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.00; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15nSAMPLEisotropicsample_conditions_1
2D 1H-1H TOCSY15nSAMPLEisotropicsample_conditions_1
2D 1H-1H NOESY15nSAMPLEisotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16574 18971
PDB
REF XP_011758823 XP_012325247
AlphaFold Q05128

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks