BMRB Entry 16574

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PDB ID: 2kpz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16574
MolProbity Validation Chart

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Title:
HUMAN NEDD4 3RD WW DOMAIN COMPLEX WITH THE HUMAN T-CELL LEUKEMIA VIRUS 1 GAG-PRO POLIPROTEIN DERIVED PEPTIDE SDPQIPPPYVEP
Deposition date:
2009-10-23
Original release date:
2015-06-19
Authors:
Iglesias-Bexiga, Manuel; Luque, Irene; Macias, Maria; Blanco, Francisco; Cobos, Eva; Bonet, Roman
Citation:

Citation: Iglesias-Bexiga, Manuel; Luque, Irene; Macias, Maria Jesus. "HUMAN NEDD4 3RD WW DOMAIN COMPLEX WITH HUMAN T-CELL LEUKEMIA VIRUS GAP-PRO POLIPROTEIN DERIVED PEPTIDE"  .

Assembly members:

Assembly members:
PROTEIN, polymer, 49 residues, 4100.688 Da.
PEPTIDE, polymer, 12 residues, 1039.193 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PROTEIN: GAMGPSEIEQGFLPKGWEVR HAPNGRPFFIDHNTKTTTWE DPRLKIPAH
PEPTIDE: SDPQIPPPYVEP

Data sets:
Data typeCount
13C chemical shifts122
15N chemical shifts46
1H chemical shifts399

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NEDD4 3RD WW1
2T-CELL LEUKEMIA VIRUS2

Entities:

Entity 1, NEDD4 3RD WW 49 residues - 4100.688 Da.

Residues 1-4 represent a non-native cloning tag resulting from TEV cleavage

1   GLYALAMETGLYPROSERGLUILEGLUGLN
2   GLYPHELEUPROLYSGLYTRPGLUVALARG
3   HISALAPROASNGLYARGPROPHEPHEILE
4   ASPHISASNTHRLYSTHRTHRTHRTRPGLU
5   ASPPROARGLEULYSILEPROALAHIS

Entity 2, T-CELL LEUKEMIA VIRUS 12 residues - 1039.193 Da.

1   SERASPPROGLNILEPROPROPROTYRVAL
2   GLUPRO

Related Database Links:

BMRB 16575 18971
PDB 2KPZ 2KQ0 2M3O 4N7F
REF XP_011758823 XP_012325247
AlphaFold P0C210

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