BMRB Entry 16445

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16445

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Title:
Backbone and side chain 1H, 13C, 15N chemical shift assignments for K2
Deposition date:
2009-08-10
Original release date:
2009-11-16
Authors:
Findlater, Emma; Steffen, Graether
Citation:

Citation: Findlater, Emma; Graether, Steffen. "NMR assignments of the intrinsically disordered K(2) and YSK (2) dehydrins"  Biomol. NMR Assignments 3, 273-275 (2009).
PubMed: 19842064

Assembly members:

Assembly members:
K2, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: frost grape   Taxonomy ID: 96939   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Vitis riparia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K2: MKEKIKERIPGMGRKDEQKQ TSATSTPGQGQQQKGMMEKI KEKLPGAH

Data sets:
Data typeCount
13C chemical shifts174
15N chemical shifts43
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K2 monomer1

Entities:

Entity 1, K2 monomer 48 residues - Formula weight is not available

1   METLYSGLULYSILELYSGLUARGILEPRO
2   GLYMETGLYARGLYSASPGLUGLNLYSGLN
3   THRSERALATHRSERTHRPROGLYGLNGLY
4   GLNGLNGLNLYSGLYMETMETGLULYSILE
5   LYSGLULYSLEUPROGLYALAHIS

Samples:

sample_1: K2, [U-100% 13C; U-100% 15N], 1.0 mM; D2O 5%; H2O 95%; NaCl 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR v2.0.7, Vim Wranken, et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16450
DBJ BAM13716 BAM13717 BAM13718 BAM13719
GB AAF37268 AAV36560 AAW58105 AAW58107 AFG16849
REF NP_001268221

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks