BMRB Entry 16450

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16450

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Title:
Backbone and side chain 1H, 13C, 15N chemical shift assignments for YSK2
Deposition date:
2009-08-17
Original release date:
2009-11-16
Authors:
Findlater, Emma; Steffen, Graether
Citation:

Citation: Findlater, Emma; Graether, Steffen. "NMR assignments of the intrinsically disordered K(2) and YSK (2) dehydrins"  Biomol. NMR Assignments 3, 273-275 (2009).
PubMed: 19842064

Assembly members:

Assembly members:
YSK2, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: eudicots   Taxonomy ID: 96939   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Vitis riparia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YSK2: MAYQQDPCANPTRQTGKTGG QTDQYGNPVHQTEALGAYGA GTGTGMHGGEHQQQPHQQPG VLNRSGSSSSEDDGQGGRRK KGMKEKIKERIPGMGRKDEQ KQTSATSTPGQGQQQKGMME KIKEKLPGAH

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts109
1H chemical shifts615

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YSK2 monomer1

Entities:

Entity 1, YSK2 monomer 130 residues - Formula weight is not available

1   METALATYRGLNGLNASPPROCYSALAASN
2   PROTHRARGGLNTHRGLYLYSTHRGLYGLY
3   GLNTHRASPGLNTYRGLYASNPROVALHIS
4   GLNTHRGLUALALEUGLYALATYRGLYALA
5   GLYTHRGLYTHRGLYMETHISGLYGLYGLU
6   HISGLNGLNGLNPROHISGLNGLNPROGLY
7   VALLEUASNARGSERGLYSERSERSERSER
8   GLUASPASPGLYGLNGLYGLYARGARGLYS
9   LYSGLYMETLYSGLULYSILELYSGLUARG
10   ILEPROGLYMETGLYARGLYSASPGLUGLN
11   LYSGLNTHRSERALATHRSERTHRPROGLY
12   GLNGLYGLNGLNGLNLYSGLYMETMETGLU
13   LYSILELYSGLULYSLEUPROGLYALAHIS

Samples:

sample_1: YSK2, [U-100% 13C; U-100% 15N], 0.6 mM; H2O 95%; D2O 5%; NaCl 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAM13716 BAM13717
GB AAF37268 AAW58105 AEP60143 AFD62287 AFG16844

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks