BMRB Entry 16304

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16304

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Title:
SLAS-bound beta-synuclein
Deposition date:
2009-05-14
Original release date:
2009-06-03
Authors:
Rao, Jampani Nageswara; Kim, Yujin; Park, Leena; Ulmer, Tobias
Citation:

Citation: Rao, Jampani Nageswara; Kim, Yujin; Park, Leena; Ulmer, Tobias. "Effect of Pseudorepeat Rearrangement on alpha-Synuclein Misfolding, Vesicle Binding, and Micelle Binding"  J. Mol. Biol. 390, 516-529 (2009).
PubMed: 19481090

Assembly members:

Assembly members:
beta-synuclein, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-42

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-synuclein: MDVFMKGLSMAKEGVVAAAE KTKQGVTEAAEKTKEGVLYV GSKTREGVVQGVASVAEKTK EQASHLGGAVFSGAGNIAAA TGLVKREEFPTDLKPEEVAQ EAAEEPLIEPLMEPEGESYE DPPQEEYQEYEPEA

Data sets:
Data typeCount
13C chemical shifts344
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 134 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERMET
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALTHRGLUALAALA
4   GLULYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRARGGLUGLYVALVALGLN
6   GLYVALALASERVALALAGLULYSTHRLYS
7   GLUGLNALASERHISLEUGLYGLYALAVAL
8   PHESERGLYALAGLYASNILEALAALAALA
9   THRGLYLEUVALLYSARGGLUGLUPHEPRO
10   THRASPLEULYSPROGLUGLUVALALAGLN
11   GLUALAALAGLUGLUPROLEUILEGLUPRO
12   LEUMETGLUPROGLUGLYGLUSERTYRGLU
13   ASPPROPROGLNGLUGLUTYRGLNGLUTYR
14   GLUPROGLUALA

Samples:

sample_1: beta-synuclein, [U-100% 13C; U-100% 15N; U-80% 2H], 0.75 mM; sodium lauroyl sarcosinate 75 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15298
DBJ BAA04610 BAG73791
EMBL CAG33308
GB AAB28945 AAB30860 AAC80286 AAH02902 AAH19409
PIR I56498
REF NP_001001502 NP_001068828 NP_001106164 NP_001248469 NP_003076
SP P33567 Q16143 Q63754 Q91ZZ3
TPG DAA27687
AlphaFold P33567 Q16143 Q63754 Q91ZZ3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks