BMRB Entry 16304

Name: SLAS-bound beta-synuclein
Published: 2009-06-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16304

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SLAS-bound beta-synuclein

Deposition date:

2009-05-14

Original release date:

2009-06-03

Authors:

Rao, Jampani Nageswara; Kim, Yujin; Park, Leena; Ulmer, Tobias

Citation:

Citation: Rao, Jampani Nageswara; Kim, Yujin; Park, Leena; Ulmer, Tobias. "Effect of Pseudorepeat Rearrangement on alpha-Synuclein Misfolding, Vesicle Binding, and Micelle Binding" J. Mol. Biol. 390, 516-529 (2009).
PubMed: 19481090

Assembly members:

Assembly members:
beta-synuclein, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-42

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-synuclein: MDVFMKGLSMAKEGVVAAAE KTKQGVTEAAEKTKEGVLYV GSKTREGVVQGVASVAEKTK EQASHLGGAVFSGAGNIAAA TGLVKREEFPTDLKPEEVAQ EAAEEPLIEPLMEPEGESYE DPPQEEYQEYEPEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	344
15N chemical shifts	122
1H chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer	1

Entities:

Entity 1, monomer 134 residues - Formula weight is not available

1

MET

ASP

VAL

PHE

MET

LYS

GLY

LEU

SER

MET

2

ALA

LYS

GLU

GLY

VAL

ALA

GLU

3

LYS

THR

LYS

GLN

GLY

VAL

THR

GLU

ALA

4

GLU

LYS

THR

LYS

GLU

GLY

VAL

LEU

TYR

VAL

5

GLY

SER

LYS

THR

ARG

GLU

GLY

VAL

GLN

6

GLY

VAL

ALA

SER

VAL

ALA

GLU

LYS

THR

LYS

7

GLU

GLN

ALA

SER

HIS

LEU

GLY

ALA

VAL

8

PHE

SER

GLY

ALA

GLY

ASN

ILE

ALA

9

THR

GLY

LEU

VAL

LYS

ARG

GLU

PHE

PRO

10

THR

ASP

LEU

LYS

PRO

GLU

VAL

ALA

GLN

11

GLU

ALA

GLU

PRO

LEU

ILE

GLU

PRO

12

LEU

MET

GLU

PRO

GLU

GLY

GLU

SER

TYR

GLU

13

ASP

PRO

GLN

GLU

TYR

GLN

GLU

TYR

14

GLU

PRO

GLU

ALA

Samples:

sample_1: beta-synuclein, [U-100% 13C; U-100% 15N; U-80% 2H], 0.75 mM; sodium lauroyl sarcosinate 75 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

BMRB	15298
DBJ	BAA04610 BAG73791
EMBL	CAG33308
GB	AAB28945 AAB30860 AAC80286 AAH02902 AAH19409
PIR	I56498
REF	NP_001001502 NP_001068828 NP_001106164 NP_001248469 NP_003076
SP	P33567 Q16143 Q63754 Q91ZZ3
TPG	DAA27687
AlphaFold	P33567 Q16143 Q63754 Q91ZZ3