BMRB Entry 15298

Title:
Beta-synuclein
Deposition date:
2007-06-11
Original release date:
2007-08-08
Authors:
Bertoncini, Carlos; Rasia, Rodolfo; Binolfi, Andres; Zweckstetter, Markus; Griesinger, Christian; Fernandez, Claudio
Citation:

Citation: Bertoncini, Carlos; Rasia, Rodolfo; Lamberto, Gonzalo; Binolfi, Andres; Zweckstetter, Markus; Griesinger, Christian; Fernandez, Claudio. "Structural characterization of the intrinsically unfolded protein beta-synuclein, a natural negative regulator of alpha-Synuclein aggregation"  J. Mol. Biol. 372, 708-722 (2007).
PubMed: 17681539

Assembly members:

Assembly members:
beta-synuclein, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PT7-7

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts124
1H chemical shifts246

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polypeptide chain1

Entities:

Entity 1, polypeptide chain 134 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERMET
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALTHRGLUALAALA
4   GLULYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRARGGLUGLYVALVALGLN
6   GLYVALALASERVALALAGLULYSTHRLYS
7   GLUGLNALASERHISLEUGLYGLYALAVAL
8   PHESERGLYALAGLYASNILEALAALAALA
9   THRGLYLEUVALLYSARGGLUGLUPHEPRO
10   THRASPLEULYSPROGLUGLUVALALAGLN
11   GLUALAALAGLUGLUPROLEUILEGLUPRO
12   LEUMETGLUPROGLUGLYGLUSERTYRGLU
13   ASPPROPROGLNGLUGLUTYRGLNGLUTYR
14   GLUPROGLUALA

Samples:

sample_1: beta-synuclein, [U-99% 13C; U-99% 15N], 400 uM; sodium chloride 100 mM; MES 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

SPARKY v3, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16304
DBJ BAA04610 BAG73791
EMBL CAG33308
GB AAB28945 AAB30860 AAC80286 AAH02902 AAH19409
PIR I56498
REF NP_001001502 NP_001068828 NP_001106164 NP_001248469 NP_003076
SP P33567 Q16143 Q63754 Q91ZZ3
TPG DAA27687
AlphaFold Q63754 P33567 Q16143 Q91ZZ3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks