BMRB Entry 15298

Name: Beta-synuclein
Published: 2007-08-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15298

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Beta-synuclein

Deposition date:

2007-06-11

Original release date:

2007-08-08

Authors:

Bertoncini, Carlos; Rasia, Rodolfo; Binolfi, Andres; Zweckstetter, Markus; Griesinger, Christian; Fernandez, Claudio

Citation:

Citation: Bertoncini, Carlos; Rasia, Rodolfo; Lamberto, Gonzalo; Binolfi, Andres; Zweckstetter, Markus; Griesinger, Christian; Fernandez, Claudio. "Structural characterization of the intrinsically unfolded protein beta-synuclein, a natural negative regulator of alpha-Synuclein aggregation" J. Mol. Biol. 372, 708-722 (2007).
PubMed: 17681539

Assembly members:

Assembly members:
beta-synuclein, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-synuclein: MDVFMKGLSMAKEGVVAAAE KTKQGVTEAAEKTKEGVLYV GSKTREGVVQGVASVAEKTK EQASHLGGAVFSGAGNIAAA TGLVKREEFPTDLKPEEVAQ EAAEEPLIEPLMEPEGESYE DPPQEEYQEYEPEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	383
15N chemical shifts	124
1H chemical shifts	246

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide chain	1

Entities:

Entity 1, polypeptide chain 134 residues - Formula weight is not available

1

MET

ASP

VAL

PHE

MET

LYS

GLY

LEU

SER

MET

2

ALA

LYS

GLU

GLY

VAL

ALA

GLU

3

LYS

THR

LYS

GLN

GLY

VAL

THR

GLU

ALA

4

GLU

LYS

THR

LYS

GLU

GLY

VAL

LEU

TYR

VAL

5

GLY

SER

LYS

THR

ARG

GLU

GLY

VAL

GLN

6

GLY

VAL

ALA

SER

VAL

ALA

GLU

LYS

THR

LYS

7

GLU

GLN

ALA

SER

HIS

LEU

GLY

ALA

VAL

8

PHE

SER

GLY

ALA

GLY

ASN

ILE

ALA

9

THR

GLY

LEU

VAL

LYS

ARG

GLU

PHE

PRO

10

THR

ASP

LEU

LYS

PRO

GLU

VAL

ALA

GLN

11

GLU

ALA

GLU

PRO

LEU

ILE

GLU

PRO

12

LEU

MET

GLU

PRO

GLU

GLY

GLU

SER

TYR

GLU

13

ASP

PRO

GLN

GLU

TYR

GLN

GLU

TYR

14

GLU

PRO

GLU

ALA

Samples:

sample_1: beta-synuclein, [U-99% 13C; U-99% 15N], 400 uM; sodium chloride 100 mM; MES 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 900 MHz

BMRB	16304
DBJ	BAA04610 BAG73791
EMBL	CAG33308
GB	AAB28945 AAB30860 AAC80286 AAH02902 AAH19409
PIR	I56498
REF	NP_001001502 NP_001068828 NP_001106164 NP_001248469 NP_003076
SP	P33567 Q16143 Q63754 Q91ZZ3
TPG	DAA27687
AlphaFold	P33567 Q16143 Q63754 Q91ZZ3