BMRB Entry 16183

Name: Tammar Wallaby Prion Protein (121-230)
Published: 2009-05-20

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PDB ID: 2kfl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16183
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Tammar Wallaby Prion Protein (121-230)

Deposition date:

2009-02-24

Original release date:

2009-05-20

Authors:

Christen, Barbara; Hornemann, Simone; Damberger, Fred; Wuthrich, Kurt

Citation:

Citation: Christen, Barbara; Hornemann, Simone; Damberger, Fred; Wuthrich, Kurt. "Prion protein NMR structure from tammar wallaby (Macropus eugenii) shows that the beta2-alpha2 loop is modulated by long-range sequence effects." J. Mol. Biol. 389, 833-845 (2009).
PubMed: 19393664

Assembly members:

Assembly members:
wpp_121-230, polymer, 112 residues, 13167.715 Da.

Natural source:

Natural source: Common Name: tammar wallaby Taxonomy ID: 9315 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Macropus Eugenii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSETA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
wpp_121-230: GSVVGGLGGYMLGSAMSRPV MHFGNEYEDRYYRENQYRYP NQVMYRPIDQYGSQNSFVHD CVNITVKQHTTTTTTKGENF TETDIKIMERVVEQMCITQY QNEYQAAQRYYN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	473
15N chemical shifts	132
1H chemical shifts	782

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	wpp_121-230	1

Entities:

Entity 1, wpp_121-230 112 residues - 13167.715 Da.

1

GLY

SER

VAL

GLY

LEU

GLY

TYR

2

MET

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

VAL

3

MET

HIS

PHE

GLY

ASN

GLU

TYR

GLU

ASP

ARG

4

TYR

ARG

GLU

ASN

GLN

TYR

ARG

TYR

PRO

5

ASN

GLN

VAL

MET

TYR

ARG

PRO

ILE

ASP

GLN

6

TYR

GLY

SER

GLN

ASN

SER

PHE

VAL

HIS

ASP

7

CYS

VAL

ASN

ILE

THR

VAL

LYS

GLN

HIS

THR

8

THR

LYS

GLY

GLU

ASN

PHE

9

THR

GLU

THR

ASP

ILE

LYS

ILE

MET

GLU

ARG

10

VAL

GLU

GLN

MET

CYS

ILE

THR

GLN

TYR

11

GLN

ASN

GLU

TYR

GLN

ALA

GLN

ARG

TYR

12

TYR

ASN

Samples:

sample_1: sodium acetate, [U-100% 2H], 10 mM; entity, [U-99% 13C; U-99% 15N], 1.5 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.01 M; pH: 4.5; pressure: 1 atm; temperature: 293.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D H(CCCO)NH	sample_1	isotropic	sample_conditions_1
3D (H)C(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - data analysis

ATHNOS-CANDID v1.2, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking

DYANA v1.0.3, Guntert, Mumenthaler and Wuthrich - structure solution

OPALP, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

Molmol, Koradi, Billeter and Wuthrich - data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 750 MHz
Bruker Avance 900 MHz

PDB	2KFL
GB	AAT68001 AAT68002