BMRB Entry 15809

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15809

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Title:
myristoylated yeast ARF1, GDP bound
Deposition date:
2008-06-18
Original release date:
2009-03-12
Authors:
Prestegard, James; Liu, Yizhou
Citation:

Citation: Liu, Yizhou; Kahn, Richard; Prestegard, James. "Structure and Membrane Interaction of Myristoylated ARF1"  Structure 17, 79-87 (2009).
PubMed: 19141284

Assembly members:

Assembly members:
myr-yARF1.GDP, polymer, 184 residues, Formula weight is not available
GDP, non-polymer, 443.201 Da.
MYR, non-polymer, 228.371 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20(b)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myr-yARF1.GDP: GLFASKLFSNLFGNKEMRIL MVGLDGAGKTTVLYKLKLGE VITTIPTIGFNVETVQYKNI SFTVWDVGGQDRIRSLWRHY YRNTEGVIFVVDSNDRSRIG EAREVMQRMLNEDELRNAAW LVFANKQDLPEAMSAAEITE KLGLHSIRNRPWFIQATCAT SGEGLYEGLEWLSNSLKNST LVRP

Data sets:
Data typeCount
13C chemical shifts525
15N chemical shifts163
1H chemical shifts529

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myr-yARF1.GDP1
2GDP2
3MYR3

Entities:

Entity 1, myr-yARF1.GDP 184 residues - Formula weight is not available

1   GLYLEUPHEALASERLYSLEUPHESERASN
2   LEUPHEGLYASNLYSGLUMETARGILELEU
3   METVALGLYLEUASPGLYALAGLYLYSTHR
4   THRVALLEUTYRLYSLEULYSLEUGLYGLU
5   VALILETHRTHRILEPROTHRILEGLYPHE
6   ASNVALGLUTHRVALGLNTYRLYSASNILE
7   SERPHETHRVALTRPASPVALGLYGLYGLN
8   ASPARGILEARGSERLEUTRPARGHISTYR
9   TYRARGASNTHRGLUGLYVALILEPHEVAL
10   VALASPSERASNASPARGSERARGILEGLY
11   GLUALAARGGLUVALMETGLNARGMETLEU
12   ASNGLUASPGLULEUARGASNALAALATRP
13   LEUVALPHEALAASNLYSGLNASPLEUPRO
14   GLUALAMETSERALAALAGLUILETHRGLU
15   LYSLEUGLYLEUHISSERILEARGASNARG
16   PROTRPPHEILEGLNALATHRCYSALATHR
17   SERGLYGLUGLYLEUTYRGLUGLYLEUGLU
18   TRPLEUSERASNSERLEULYSASNSERTHR
19   LEUVALARGPRO

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, MYR - C14 H28 O2 - 228.371 Da.

1   MYR

Samples:

sample_2: myr-yARF1.GDP, [U-13C; U-15N; Methyl-1H;U-2H;], 0.8 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_3: myr-yARF1.GDP, [U-13C; U-15N; U-2H], 0.5 mM; KH2PO4-K2HPO4 50 mM; NaCl 50 mM; K2SO4 10 mM; MgCl2 2 mM; DTT 2 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_1: myr-yARF1.GDP, [U-13C; U-15N; U-2H], 0.7 mM; KH2PO4-K2HPO4 50 mM; NaCl 50 mM; K2SO4 10 mM; MgCl2 2 mM; DTT 2 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 13C-filtered-13C-edited NOESYsample_2isotropicsample_conditions_1
3D CCmHm-TOCSYsample_2isotropicsample_conditions_1
HSQC-TROSYsample_3anisotropicsample_conditions_1
HNCO-JNHsample_3anisotropicsample_conditions_1
HNCO-JNCOsample_3anisotropicsample_conditions_1
HSQC-TROSYsample_2isotropicsample_conditions_1
HNCO-JNHsample_2isotropicsample_conditions_1
HNCO-JNCOsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Zhengrong and Bax - processing

SPARKY, Goddard - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 15626
PDB
DBJ GAA22060
EMBL CAA58255 CAA98769 CAG60356 CAY78316 CCC67945
GB AAA34431 AHY74824 AJP37565 AJU57677 AJU58380
REF NP_010089 XP_001646910 XP_003667691 XP_003674325 XP_003676799
SP P11076
TPG DAA11671
AlphaFold P11076

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks