BMRB Entry 15626

Name: backbone assignment of yeast (Saccharomyces cerevisiae) ARF1 ,non-myristoylated, GDP-bound
Published: 2008-06-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15626

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

backbone assignment of yeast (Saccharomyces cerevisiae) ARF1 ,non-myristoylated, GDP-bound

Deposition date:

2008-01-15

Original release date:

2008-06-30

Authors:

Liu, Yizhou

Citation:

Citation: Liu, Yizhou; Prestegard, James. "Direct measurement of dipole-dipole/CSA cross-correlated relaxation by a constant-time experiment" J. Magn. Reson. 193, 23-31 (2008).
PubMed: 18406649

Assembly members:

Assembly members:
yeast_ARF1, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yeast_ARF1: GLFASKLFSNLFGNKEMRIL MVGLDGAGKTTVLYKLKLGE VITTIPTIGFNVETVQYKNI SFTVWDVGGQDRIRSLWRHY YRNTEGVIFVVDSNDRSRIG EAREVMQRMLNEDELRNAAW LVFANKQDLPEAMSAAEITE KLGLHSIRNRPWFIQATCAT SGEGLYEGLEWLSNSLKNST LEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	299
15N chemical shifts	155
1H chemical shifts	155

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yeast ARF1	1

Entities:

Entity 1, yeast ARF1 188 residues - Formula weight is not available

1

GLY

LEU

PHE

ALA

SER

LYS

LEU

PHE

SER

ASN

2

LEU

PHE

GLY

ASN

LYS

GLU

MET

ARG

ILE

LEU

3

MET

VAL

GLY

LEU

ASP

GLY

ALA

GLY

LYS

THR

4

THR

VAL

LEU

TYR

LYS

LEU

LYS

LEU

GLY

GLU

5

VAL

ILE

THR

ILE

PRO

THR

ILE

GLY

PHE

6

ASN

VAL

GLU

THR

VAL

GLN

TYR

LYS

ASN

ILE

7

SER

PHE

THR

VAL

TRP

ASP

VAL

GLY

GLN

8

ASP

ARG

ILE

ARG

SER

LEU

TRP

ARG

HIS

TYR

9

TYR

ARG

ASN

THR

GLU

GLY

VAL

ILE

PHE

VAL

10

VAL

ASP

SER

ASN

ASP

ARG

SER

ARG

ILE

GLY

11

GLU

ALA

ARG

GLU

VAL

MET

GLN

ARG

MET

LEU

12

ASN

GLU

ASP

GLU

LEU

ARG

ASN

ALA

TRP

13

LEU

VAL

PHE

ALA

ASN

LYS

GLN

ASP

LEU

PRO

14

GLU

ALA

MET

SER

ALA

GLU

ILE

THR

GLU

15

LYS

LEU

GLY

LEU

HIS

SER

ILE

ARG

ASN

ARG

16

PRO

TRP

PHE

ILE

GLN

ALA

THR

CYS

ALA

THR

17

SER

GLY

GLU

GLY

LEU

TYR

GLU

GLY

LEU

GLU

18

TRP

LEU

SER

ASN

SER

LEU

LYS

ASN

SER

THR

19

LEU

GLU

HIS

Samples:

sample_1: yeast ARF1, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

VNMRJ, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	15809
PDB	2K5U 2KSQ 3TJZ 4Q66 5A1U 5A1V 5A1W 5A1X 5A1Y
DBJ	GAA22060
EMBL	CAA58255 CAA98769 CAG60356 CAY78316 CCC67945
GB	AAA34431 AHY74824 AJP37565 AJU57677 AJU58380
REF	NP_010089 XP_001646910 XP_003667691 XP_003674325 XP_003676799
SP	P11076
TPG	DAA11671
AlphaFold	P11076