BMRB Entry 15606
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15606
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Babon, Jeffrey; Sabo, Jennifer; Soetopo, Alfreda; Yao, Shenggen; Bailey, Micheal; Zhang, Jian-Guo; Nicola, Nicos; Norton, Raymond. "The SOCS Box Domain of SOCS3: Structure and Interaction with the ElonginBC-Cullin5 Ubiquitin Ligase" J. Mol. Biol. 381, 928-940 (2008).
PubMed: 18590740
Assembly members:
socs_box, polymer, 40 residues, Formula weight is not available
elonginB, polymer, 118 residues, Formula weight is not available
elonginC, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T
Entity Sequences (FASTA):
socs_box: VATLQHLCRKTVNGHLDSYE
KVTQLPGPIREFLDQYDAPL
elonginB: MDVFLMIRRHKTTIFTDAKE
SSTVFELKRIVEGILKRPPD
EQRLYKDDQLLDDGKTLGEC
GFTSQTARPQAPATVGLAFR
ADDTFEALCIEPFSSPPELP
DVMKPQDSGSSANEQAVQ
elonginC: MYVKLISSDGHEFIVKREHA
LTSGTIKAMLSGPGQFAENE
TNEVNFREIPSHVLSKVCMY
FTYKVRYTNSSTEIPEFPIA
PEIALELLMAANFLDC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 639 |
| 15N chemical shifts | 202 |
| 1H chemical shifts | 741 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chain a | 1 |
| 2 | chain b | 2 |
| 3 | chain c | 3 |
Entities:
Entity 1, chain a 40 residues - Formula weight is not available
| 1 | VAL | ALA | THR | LEU | GLN | HIS | LEU | CYS | ARG | LYS | |
| 2 | THR | VAL | ASN | GLY | HIS | LEU | ASP | SER | TYR | GLU | |
| 3 | LYS | VAL | THR | GLN | LEU | PRO | GLY | PRO | ILE | ARG | |
| 4 | GLU | PHE | LEU | ASP | GLN | TYR | ASP | ALA | PRO | LEU |
Entity 2, chain b 118 residues - Formula weight is not available
| 1 | MET | ASP | VAL | PHE | LEU | MET | ILE | ARG | ARG | HIS | ||||
| 2 | LYS | THR | THR | ILE | PHE | THR | ASP | ALA | LYS | GLU | ||||
| 3 | SER | SER | THR | VAL | PHE | GLU | LEU | LYS | ARG | ILE | ||||
| 4 | VAL | GLU | GLY | ILE | LEU | LYS | ARG | PRO | PRO | ASP | ||||
| 5 | GLU | GLN | ARG | LEU | TYR | LYS | ASP | ASP | GLN | LEU | ||||
| 6 | LEU | ASP | ASP | GLY | LYS | THR | LEU | GLY | GLU | CYS | ||||
| 7 | GLY | PHE | THR | SER | GLN | THR | ALA | ARG | PRO | GLN | ||||
| 8 | ALA | PRO | ALA | THR | VAL | GLY | LEU | ALA | PHE | ARG | ||||
| 9 | ALA | ASP | ASP | THR | PHE | GLU | ALA | LEU | CYS | ILE | ||||
| 10 | GLU | PRO | PHE | SER | SER | PRO | PRO | GLU | LEU | PRO | ||||
| 11 | ASP | VAL | MET | LYS | PRO | GLN | ASP | SER | GLY | SER | ||||
| 12 | SER | ALA | ASN | GLU | GLN | ALA | VAL | GLN |
Entity 3, chain c 96 residues - Formula weight is not available
| 1 | MET | TYR | VAL | LYS | LEU | ILE | SER | SER | ASP | GLY | ||||
| 2 | HIS | GLU | PHE | ILE | VAL | LYS | ARG | GLU | HIS | ALA | ||||
| 3 | LEU | THR | SER | GLY | THR | ILE | LYS | ALA | MET | LEU | ||||
| 4 | SER | GLY | PRO | GLY | GLN | PHE | ALA | GLU | ASN | GLU | ||||
| 5 | THR | ASN | GLU | VAL | ASN | PHE | ARG | GLU | ILE | PRO | ||||
| 6 | SER | HIS | VAL | LEU | SER | LYS | VAL | CYS | MET | TYR | ||||
| 7 | PHE | THR | TYR | LYS | VAL | ARG | TYR | THR | ASN | SER | ||||
| 8 | SER | THR | GLU | ILE | PRO | GLU | PHE | PRO | ILE | ALA | ||||
| 9 | PRO | GLU | ILE | ALA | LEU | GLU | LEU | LEU | MET | ALA | ||||
| 10 | ALA | ASN | PHE | LEU | ASP | CYS |
Samples:
sample_1: socs box, [U-100% 13C; U-100% 15N], 1 mM; elonginB 1 mM; elonginC 1 mM; potassium chloride 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.7; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
TALOS, Cornilescu, Delaglio and Bax - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA22430 BAA22537 BAC32977 BAD42435 BAE23929 BAB21946 BAB22417 BAB22684 BAB25690 BAB25727 BAB27825 BAB27939 BAB28546 BAB28641 BAC30437 |
| EMBL | CAB56083 CAG38736 CAG46495 CAL37774 CAL38024 CAG32006 CAJ81641 CBN81906 CDQ94726 CDQ97243 |
| GB | AAB62403 AAC26223 AAD42231 AAH52031 AAH60858 AAA75522 AAA80968 AAC08452 AAH13306 AAH51927 AAA41109 AAA67650 AAH09104 AAH13809 AAH28545 |
| REF | NP_001026801 NP_001116668 NP_001116851 NP_001181255 NP_003946 NP_001029972 NP_009039 NP_080581 NP_112391 NP_996896 NP_001002440 NP_001004673 NP_001007889 NP_001016420 NP_001039958 |
| SP | O14543 O35718 O88583 Q68AM8 Q9BEG9 P62869 P62870 Q15370 P83940 P83941 Q15369 Q2KII4 |
| TPG | DAA18184 DAA15620 DAA22672 |
| BMRB | 19333 19333 |
| PRF | 2119399A 2119399B |
| AlphaFold | O14543 O35718 O88583 Q68AM8 Q9BEG9 P62869 P62870 Q15370 P83940 P83941 Q15369 Q2KII4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks