BMRB Entry 15202

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15202

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Title:
Rub184b
Deposition date:
2007-04-04
Original release date:
2007-08-23
Authors:
Schwarten, Melanie; Berghaus, Carsten; Heumann, Rolf; Stoll, Raphael
Citation:

Citation: Schwarten, Melanie; Berghaus, Carsten; Heumann, Rolf; Stoll, Raphael. "Sequence-specific 1H, 13C, and 15N backbone assignment of the activated 21 kDa GTPase rRheb"  Biomol. NMR Assignments 1, 105-108 (2007).
PubMed: 19636840

Assembly members:

Assembly members:
Rub184_GppNHp, polymer, 184 residues, Formula weight is not available
GNP, non-polymer, Formula weight is not available
MG, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rub184_GppNHp: SPQSKSRKIAILGYRSVGKS SLTIQFVEGQFVDSYDPTIE NTFTKLITVNGQEYHLQLVD TAGQDEYSIFPQTYSIDING YILVYSVTSIKSFEVIKVIH GKLLDMVGKVQIPIMLVGNK KDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVF RRIILEAEKIDGAASQGKSS CSVM

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts131
1H chemical shifts243

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rub184_GppNHp1
2GppNHp2
3Mg2+3

Entities:

Entity 1, Rub184_GppNHp 184 residues - Formula weight is not available

1   SERPROGLNSERLYSSERARGLYSILEALA
2   ILELEUGLYTYRARGSERVALGLYLYSSER
3   SERLEUTHRILEGLNPHEVALGLUGLYGLN
4   PHEVALASPSERTYRASPPROTHRILEGLU
5   ASNTHRPHETHRLYSLEUILETHRVALASN
6   GLYGLNGLUTYRHISLEUGLNLEUVALASP
7   THRALAGLYGLNASPGLUTYRSERILEPHE
8   PROGLNTHRTYRSERILEASPILEASNGLY
9   TYRILELEUVALTYRSERVALTHRSERILE
10   LYSSERPHEGLUVALILELYSVALILEHIS
11   GLYLYSLEULEUASPMETVALGLYLYSVAL
12   GLNILEPROILEMETLEUVALGLYASNLYS
13   LYSASPLEUHISMETGLUARGVALILESER
14   TYRGLUGLUGLYLYSALALEUALAGLUSER
15   TRPASNALAALAPHELEUGLUSERSERALA
16   LYSGLUASNGLNTHRALAVALASPVALPHE
17   ARGARGILEILELEUGLUALAGLULYSILE
18   ASPGLYALAALASERGLNGLYLYSSERSER
19   CYSSERVALMET

Entity 2, GppNHp - Formula weight is not available

1   GNP

Entity 3, Mg2+ - Formula weight is not available

1   MG

Samples:

Rub184_GppNHp_15N: GTP-binding protein, [U-15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 10 mM; magnesium chloride 5 mM; DTT 1 mM; TSP 1 mM; H2O 90%; D2O 10%

Rub184_GppNHp_15N13C: GTP-binding protein, [U-13C; U-15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 10 mM; magnesium chloride 5 mM; DTT 1 mM; TSP 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCRub184_GppNHp_15Nisotropicsample_conditions_1
3D HNCARub184_GppNHp_15N13Cisotropicsample_conditions_1
3D HN(CO)CARub184_GppNHp_15N13Cisotropicsample_conditions_1
3D CBCA(CO)NHRub184_GppNHp_15N13Cisotropicsample_conditions_1
3D HNHARub184_GppNHp_15N13Cisotropicsample_conditions_1
3D 1H-15N NOESYRub184_GppNHp_15Nisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15048
PDB
DBJ BAA11211 BAB13483 BAE30208 BAF82059 BAG54198
EMBL CAA82774
GB AAA21380 AAD15548 AAF73125 AAH12273 AAH16155
REF NP_001026934 NP_001155338 NP_001232539 NP_001248111 NP_001272585
SP Q15382 Q56JV3 Q62639 Q921J2
TPG DAA25358 DAA30304
AlphaFold Q15382 Q56JV3 Q62639 Q921J2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks