BMRB Entry 15048

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15048

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Title:
Rub184
Deposition date:
2006-11-21
Original release date:
2007-11-21
Authors:
Berghaus, Carsten; Schwarten, Melanie; Heumann, Rolf; Stoll, Raphael
Citation:

Citation: Berghaus, Carsten; Schwarten, Melanie; Heumann, Rolf; Stoll, Raphael. "Sequence-specific 1H, 13C, and 15N backbone assignment of the GTPase rRheb in its GDP-bound form"  Biomol. NMR Assignments 1, 45-47 (2007).
PubMed: 19636822

Assembly members:

Assembly members:
Rub184, polymer, 184 residues, Formula weight is not available
GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rub184: SPQSKSRKIAILGYRSVGKS SLTIQFVEGQFVDSYDPTIE NTFTKLITVNGQEYHLQLVD TAGQDEYSIFPQTYSIDING YILVYSVTSIKSFEVIKVIH GKLLDMVGKVQIPIMLVGNK KDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVF RRIILEAEKIDGAASQGKSS CSVM

Data sets:
Data typeCount
13C chemical shifts528
15N chemical shifts167
1H chemical shifts881

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rub1841
2nucleotide2

Entities:

Entity 1, Rub184 184 residues - Formula weight is not available

1   SERPROGLNSERLYSSERARGLYSILEALA
2   ILELEUGLYTYRARGSERVALGLYLYSSER
3   SERLEUTHRILEGLNPHEVALGLUGLYGLN
4   PHEVALASPSERTYRASPPROTHRILEGLU
5   ASNTHRPHETHRLYSLEUILETHRVALASN
6   GLYGLNGLUTYRHISLEUGLNLEUVALASP
7   THRALAGLYGLNASPGLUTYRSERILEPHE
8   PROGLNTHRTYRSERILEASPILEASNGLY
9   TYRILELEUVALTYRSERVALTHRSERILE
10   LYSSERPHEGLUVALILELYSVALILEHIS
11   GLYLYSLEULEUASPMETVALGLYLYSVAL
12   GLNILEPROILEMETLEUVALGLYASNLYS
13   LYSASPLEUHISMETGLUARGVALILESER
14   TYRGLUGLUGLYLYSALALEUALAGLUSER
15   TRPASNALAALAPHELEUGLUSERSERALA
16   LYSGLUASNGLNTHRALAVALASPVALPHE
17   ARGARGILEILELEUGLUALAGLULYSILE
18   ASPGLYALAALASERGLNGLYLYSSERSER
19   CYSSERVALMET

Entity 2, nucleotide - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Samples:

Rub184_15N: GTP-binding protein, [U-15N], 0.9 – 1.2 mM; D2O 10%; potassium phosphate 20 mM; H2O 90%; DTT 1 mM; GUANOSINE-5'-DIPHOSPHATE0.9 – 1.2 mM; sodium chloride 10 mM; TSP 1 mM; magnesium chloride 5 mM

Rub184_15N13C: GTP-binding protein, [U-13C; U-15N], 0.9 – 1.2 mM; GUANOSINE-5'-DIPHOSPHATE0.9 – 1.2 mM; D2O 10%; H2O 90%; DTT 1 mM; sodium chloride 10 mM; potassium phosphate 20 mM; TSP 1 mM; magnesium chloride 5 mM

Rub184_15N_conditions: pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCRub184_15NisotropicRub184_15N_conditions
3D HNCORub184_15N13CisotropicRub184_15N_conditions
3D HNCARub184_15N13CisotropicRub184_15N_conditions
3D HN(CO)CARub184_15N13CisotropicRub184_15N_conditions
3D HNHARub184_15NisotropicRub184_15N_conditions
3D HNHBRub184_15NisotropicRub184_15N_conditions
3D HBHA(CO)NHRub184_15N13CisotropicRub184_15N_conditions
3D 1H-15N NOESYRub184_15NisotropicRub184_15N_conditions

Software:

NMRPipe, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - processing

NMRDraw, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - data analysis

NMRView, B Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

BMRB 15202
PDB
DBJ BAA11211 BAB13483 BAE30208 BAF82059 BAG54198
EMBL CAA82774
GB AAA21380 AAD15548 AAF73125 AAH12273 AAH16155
REF NP_001026934 NP_001155338 NP_001232539 NP_001248111 NP_001272585
SP Q15382 Q56JV3 Q62639 Q921J2
TPG DAA25358 DAA30304
AlphaFold Q15382 Q56JV3 Q62639 Q921J2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks