BMRB Entry 15143

Title:
1H (NH, HA, HB) Chemical Shift Assignments of the Non-Specific Lipid Transfer Protein (nsLTP1) with post translational modification, isolated from Barley Seeds
Deposition date:
2007-02-24
Original release date:
2007-10-30
Authors:
Wijesinha-Bettoni, Ramani; Gao, Chunli; Jenkins, John; Mackie, Alan; Wilde, Peter; Mills, E. N; Smith, Lorna
Citation:

Citation: Wijesinha-Bettoni, Ramani; Gao, Chunli; Jenkins, John; Mackie, Alan; Wilde, Peter; Mills, E. N; Smith, Lorna. "Post-translational modification of barley LTP1b: the lipid adduct lies in the hydrophobic cavity and alters the protein dynamics"  FEBS Lett. 581, 4557-4561 (2007).
PubMed: 17854802

Assembly members:

Assembly members:
nsLTP1b, polymer, 91 residues, Formula weight is not available
CHDA, non-polymer, 294.3 Da.

Natural source:

Natural source:   Common Name: barley   Taxonomy ID: 4513   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Hordeum vulgare

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount
1H chemical shifts279

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barley lipid transfer protein with post-transaltional modification1
2cis-7-heptadecenoic acid2

Entities:

Entity 1, barley lipid transfer protein with post-transaltional modification 91 residues - Formula weight is not available

1   LEUASNCYSGLYGLNVALASPSERLYSMET
2   LYSPROCYSLEUTHRTYRVALGLNGLYGLY
3   PROGLYPROSERGLYGLUCYSCYSASNGLY
4   VALARGASPLEUHISASNGLNALAGLNSER
5   SERGLYASPARGGLNTHRVALCYSASNCYS
6   LEULYSGLYILEALAARGGLYILEHISASN
7   LEUASNLEUASNASNALAALASERILEPRO
8   SERLYSCYSASNVALASNVALPROTYRTHR
9   ILESERPROASPILEASPCYSSERARGILE
10   TYR

Entity 2, cis-7-heptadecenoic acid - C17 H28 05 - 294.3 Da.

1   CHDA

Samples:

sample_1: nsLTP1b 4 ± 0.2 mM

sample_conditions_1: pH: 4.0; temperature: 310 K

sample_conditions_2: pH: 3; temperature: 299 K

sample_conditions_3: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_3
2D 1H-1H NOESYsample_1isotropicsample_conditions_3

Software:

SPARKY vVersion 3.110, Goddard - data analysis

NMR spectrometers:

  • Home-built . 600 MHz

Related Database Links:

BMRB 4142
PDB
DBJ BAK05317
EMBL CAA28805 CAA41946 CAA42832 CAP16136
GB AAA32970
SP P07597
AlphaFold P07597