BMRB Entry 15143

Name: 1H (NH, HA, HB) Chemical Shift Assignments of the Non-Specific Lipid Transfer Protein (nsLTP1) with post translational modification, isolated from Barley Seeds
Published: 2007-10-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15143

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H (NH, HA, HB) Chemical Shift Assignments of the Non-Specific Lipid Transfer Protein (nsLTP1) with post translational modification, isolated from Barley Seeds

Deposition date:

2007-02-24

Original release date:

2007-10-30

Authors:

Wijesinha-Bettoni, Ramani; Gao, Chunli; Jenkins, John; Mackie, Alan; Wilde, Peter; Mills, E. N; Smith, Lorna

Citation:

Citation: Wijesinha-Bettoni, Ramani; Gao, Chunli; Jenkins, John; Mackie, Alan; Wilde, Peter; Mills, E. N; Smith, Lorna. "Post-translational modification of barley LTP1b: the lipid adduct lies in the hydrophobic cavity and alters the protein dynamics" FEBS Lett. 581, 4557-4561 (2007).
PubMed: 17854802

Assembly members:

Assembly members:
nsLTP1b, polymer, 91 residues, Formula weight is not available
CHDA, non-polymer, 294.3 Da.

Natural source:

Natural source: Common Name: barley Taxonomy ID: 4513 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Hordeum vulgare

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nsLTP1b: LNCGQVDSKMKPCLTYVQGG PGPSGECCNGVRDLHNQAQS SGDRQTVCNCLKGIARGIHN LNLNNAASIPSKCNVNVPYT ISPDIDCSRIY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	279

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	barley lipid transfer protein with post-transaltional modification	1
2	cis-7-heptadecenoic acid	2

Entities:

Entity 1, barley lipid transfer protein with post-transaltional modification 91 residues - Formula weight is not available

1

LEU

ASN

CYS

GLY

GLN

VAL

ASP

SER

LYS

MET

2

LYS

PRO

CYS

LEU

THR

TYR

VAL

GLN

GLY

3

PRO

GLY

PRO

SER

GLY

GLU

CYS

ASN

GLY

4

VAL

ARG

ASP

LEU

HIS

ASN

GLN

ALA

GLN

SER

5

SER

GLY

ASP

ARG

GLN

THR

VAL

CYS

ASN

CYS

6

LEU

LYS

GLY

ILE

ALA

ARG

GLY

ILE

HIS

ASN

7

LEU

ASN

LEU

ASN

ALA

SER

ILE

PRO

8

SER

LYS

CYS

ASN

VAL

ASN

VAL

PRO

TYR

THR

9

ILE

SER

PRO

ASP

ILE

ASP

CYS

SER

ARG

ILE

10

TYR

Entity 2, cis-7-heptadecenoic acid - C17 H28 05 - 294.3 Da.

1

CHDA

Samples:

sample_1: nsLTP1b 4 ± 0.2 mM

sample_conditions_1: pH: 4.0; temperature: 310 K

sample_conditions_2: pH: 3; temperature: 299 K

sample_conditions_3: pH: 5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_3
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_3

Software:

SPARKY vVersion 3.110, Goddard - data analysis

NMR spectrometers:

Home-built . 600 MHz

BMRB	4142
PDB	1BE2 1JTB 1LIP 1MID 3GSH
DBJ	BAK05317
EMBL	CAA28805 CAA41946 CAA42832 CAP16136
GB	AAA32970
SP	P07597
AlphaFold	P07597