BMRB Entry 15073

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15073

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Title:
NMR assignments of CO-bound ferrous Corynebacterium diphtheria Heme Oxygenase
Deposition date:
2006-12-12
Original release date:
2007-06-26
Authors:
Hom, Kellie; Deshmukh, Rahul; Furci, Lena; Wilks, Angela
Citation:

Citation: Hom, Kellie; Deshmukh, Rahul; Furci, Lena; Wilks, Angela. "NMR assignments of cd-HO, a 24 kDa heme oxygenase from Corynebacterium diphtheria"  Biomol. NMR Assignments 1, 55-56 (2007).
PubMed: 19636825

Assembly members:

Assembly members:
heme_oxygenase, polymer, 215 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Corynebacterium diphtheria   Taxonomy ID: 1717   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium diphtheriae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
heme_oxygenase: MTTATAGLAVELKQSTAQAH EKAEHSTFMSDLLKGRLGVA EFTRLQEQAWLFYTALEQAV DAVRASGFAESLLDPALNRA EVLARDLDKLNGSSEWRSRI TASPAVIDYVNRLEEIRDNV DGPALVAHHYVRYLGDLSGG QVIARMMQRHYGVDPEALGF YHFEGIAKLKVYKDEYREKL NNLELSDEQREHLLKEATDA FVFNHQVFADLGKGL

Data sets:
Data typeCount
13C chemical shifts472
15N chemical shifts163
1H chemical shifts163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1heme oxygenase1
2ferrous heme2

Entities:

Entity 1, heme oxygenase 215 residues - Formula weight is not available

1   METTHRTHRALATHRALAGLYLEUALAVAL
2   GLULEULYSGLNSERTHRALAGLNALAHIS
3   GLULYSALAGLUHISSERTHRPHEMETSER
4   ASPLEULEULYSGLYARGLEUGLYVALALA
5   GLUPHETHRARGLEUGLNGLUGLNALATRP
6   LEUPHETYRTHRALALEUGLUGLNALAVAL
7   ASPALAVALARGALASERGLYPHEALAGLU
8   SERLEULEUASPPROALALEUASNARGALA
9   GLUVALLEUALAARGASPLEUASPLYSLEU
10   ASNGLYSERSERGLUTRPARGSERARGILE
11   THRALASERPROALAVALILEASPTYRVAL
12   ASNARGLEUGLUGLUILEARGASPASNVAL
13   ASPGLYPROALALEUVALALAHISHISTYR
14   VALARGTYRLEUGLYASPLEUSERGLYGLY
15   GLNVALILEALAARGMETMETGLNARGHIS
16   TYRGLYVALASPPROGLUALALEUGLYPHE
17   TYRHISPHEGLUGLYILEALALYSLEULYS
18   VALTYRLYSASPGLUTYRARGGLULYSLEU
19   ASNASNLEUGLULEUSERASPGLUGLNARG
20   GLUHISLEULEULYSGLUALATHRASPALA
21   PHEVALPHEASNHISGLNVALPHEALAASP
22   LEUGLYLYSGLYLEU

Entity 2, ferrous heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: heme oxygenase, [U-13C; U-15N], 1 – 2 mM; PROTOPORPHYRIN IX CONTAINING FE1 – 2 mM; H2O 95%; D2O, [U-2H], 5%; potassium chloride 100 mM; potassium phosphate 50 mM; DSS 0.5 mM; CO1 – 2 mM; sodium dithionite2 – 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.11, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 15070 15071
PDB
DBJ BAA76407
EMBL CAE50198 CKH06070
GB AAC44832 AEX42338 AEX44660 AEX46855 AEX67845
REF WP_003852259 WP_010935240 WP_014302152 WP_014303664 WP_014307115
SP P71119
AlphaFold P71119

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks