BMRB Entry 15070

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15070

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Title:
NMR Assignments of the apo Corynebacterium diphtheria Heme Oxygenase-G135A
Deposition date:
2006-12-07
Original release date:
2007-06-26
Authors:
Hom, Kellie
Citation:

Citation: Hom, Kellie; Furci, Lena; Deshmukh, Rahul; Wilks, Angela. "NMR assignments of cd-HO, a 24 kDa heme oxygenase from Corynebacterium diphtheria"  Biomol. NMR Assignments 1, 55-56 (2007).
PubMed: 19636825

Assembly members:

Assembly members:
heme_oxygenase, polymer, 215 residues, 24152 Da.

Natural source:

Natural source:   Common Name: Corynebacterium diphtheria   Taxonomy ID: 1717   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium diphtheriae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
heme_oxygenase: MTTATAGLAVELKQSTAQAH EKAEHSTFMSDLLKGRLGVA EFTRLQEQAWLFYTALEQAV DAVRASGFAESLLDPALNRA EVLARDLDKLNGSSEWRSRI TASPAVIDYVNRLEEIRDNV DGPALVAHHYVRYLADLSGG QVIARMMQRHYGVDPEALGF YHFEGIAKLKVYKDEYREKL NNLELSDEQREHLLKEATDA FVFNHQVFADLGKGL

Data sets:
Data typeCount
13C chemical shifts571
15N chemical shifts190
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1heme oxygenase1

Entities:

Entity 1, heme oxygenase 215 residues - 24152 Da.

1   METTHRTHRALATHRALAGLYLEUALAVAL
2   GLULEULYSGLNSERTHRALAGLNALAHIS
3   GLULYSALAGLUHISSERTHRPHEMETSER
4   ASPLEULEULYSGLYARGLEUGLYVALALA
5   GLUPHETHRARGLEUGLNGLUGLNALATRP
6   LEUPHETYRTHRALALEUGLUGLNALAVAL
7   ASPALAVALARGALASERGLYPHEALAGLU
8   SERLEULEUASPPROALALEUASNARGALA
9   GLUVALLEUALAARGASPLEUASPLYSLEU
10   ASNGLYSERSERGLUTRPARGSERARGILE
11   THRALASERPROALAVALILEASPTYRVAL
12   ASNARGLEUGLUGLUILEARGASPASNVAL
13   ASPGLYPROALALEUVALALAHISHISTYR
14   VALARGTYRLEUALAASPLEUSERGLYGLY
15   GLNVALILEALAARGMETMETGLNARGHIS
16   TYRGLYVALASPPROGLUALALEUGLYPHE
17   TYRHISPHEGLUGLYILEALALYSLEULYS
18   VALTYRLYSASPGLUTYRARGGLULYSLEU
19   ASNASNLEUGLULEUSERASPGLUGLNARG
20   GLUHISLEULEULYSGLUALATHRASPALA
21   PHEVALPHEASNHISGLNVALPHEALAASP
22   LEUGLYLYSGLYLEU

Samples:

sample_1: heme oxygenase, [U-13C; U-15N; U-2H], 1 – 2 mM; H2O 95%; D2O 5%; potassium phosphate 50 mM; potassium chloride 100 mM; DSS 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.11, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 15071 15073
PDB
DBJ BAA76407
EMBL CAE50198 CKH06070
GB AAC44832 AEX42338 AEX44660 AEX46855 AEX67845
REF WP_003852259 WP_010935240 WP_014302152 WP_014303664 WP_014307115
SP P71119
AlphaFold P71119

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks