BMRB Entry 11209

Name: Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)
Published: 2011-07-21

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PDB ID: 1x43
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11209
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)" .

Assembly members:

Assembly members:
SH3 domain, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041220-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGLNDLKESSNNRKA RVLYDYDAANSTELSLLADE VITVFSVVGMDSDWLMGERG NQKGKVPITYLELLNSGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	326
15N chemical shifts	74
1H chemical shifts	518

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain GRB2-like protein B1	1

Entities:

Entity 1, SH3 domain GRB2-like protein B1 81 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LEU

ASN

ASP

2

LEU

LYS

GLU

SER

ASN

ARG

LYS

ALA

3

ARG

VAL

LEU

TYR

ASP

TYR

ASP

ALA

ASN

4

SER

THR

GLU

LEU

SER

LEU

ALA

ASP

GLU

5

VAL

ILE

THR

VAL

PHE

SER

VAL

GLY

MET

6

ASP

SER

ASP

TRP

LEU

MET

GLY

GLU

ARG

GLY

7

ASN

GLN

LYS

GLY

LYS

VAL

PRO

ILE

THR

TYR

8

LEU

GLU

LEU

ASN

SER

GLY

PRO

SER

9

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.52 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, DelaglioF. - processing

NMRView v5.0.4, JohnsonB.A. - data analysis

Kujira v0.9296, KobayashiN. - data analysis

CYANA v2.0.17, GuntertP. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1X43
DBJ	BAE34495
GB	ABA54268 EDL12028
REF	NP_001268971