Chemical shift reference of 1H was based on the proton of water (4.784ppm at 298K) and then those of 15N and 13C were calculated based on their gyromagnetic ratios.

Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)

endophilin B1 testis variant [Mus musculus]

SH3-domain GRB2-like B1 (endophilin), isoform CRA_c, partial [Mus musculus]

1.52mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax