BMRB Entry 11177

Name: Backbone chemical shift assignments for Thermus thermophilus RecO
Published: 2011-06-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11177

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments for Thermus thermophilus RecO

Deposition date:

2010-07-08

Original release date:

2011-06-03

Authors:

Inoue, Jin; Nagae, Takayuki; Mishima, Masaki; Ito, Yutaka; Shibata, Takehiko; Mikawa, Tsutomu

Citation:

Citation: Inoue, Jin; Nagae, Takayuki; Mishima, Masaki; Ito, Yutaka; Shibata, Takehiko; Mikawa, Tsutomu. "A mechanism for single-stranded DNA-binding protein (SSB) displacement from single-stranded DNA upon SSB-RecO interaction." J. Biol. Chem. 286, 6720-6732 (2011).
PubMed: 21169364

Assembly members:

Assembly members:
RecO, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thermus thermophilus HB8 Taxonomy ID: 300852 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RecO: MAPAYTGKVERYRLEEGIVV GRKPLPQGDLLLRLVTPRGS LEAVVRKGQRPTGRTGRLSL FHHVRFQLYAKGEGLPTLTQ AELLGRLHGLEAPRRFLLAA FLAELAYRLASPEAAPRIYP LLVSGLRGIAKHEDPLLPLV WAGWRVAKAGGIGPNLEGEG LRLKRGRLGEEGVYLGREGV EALKATLRLPGAQALPHLEG APLNRLFLALKAHAEEALGP LRSAEAIGV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	189
1H chemical shifts	189

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RecO	1

Entities:

Entity 1, RecO 229 residues - Formula weight is not available

1

MET

ALA

PRO

ALA

TYR

THR

GLY

LYS

VAL

GLU

2

ARG

TYR

ARG

LEU

GLU

GLY

ILE

VAL

3

GLY

ARG

LYS

PRO

LEU

PRO

GLN

GLY

ASP

LEU

4

LEU

ARG

LEU

VAL

THR

PRO

ARG

GLY

SER

5

LEU

GLU

ALA

VAL

ARG

LYS

GLY

GLN

ARG

6

PRO

THR

GLY

ARG

THR

GLY

ARG

LEU

SER

LEU

7

PHE

HIS

VAL

ARG

PHE

GLN

LEU

TYR

ALA

8

LYS

GLY

GLU

GLY

LEU

PRO

THR

LEU

THR

GLN

9

ALA

GLU

LEU

GLY

ARG

LEU

HIS

GLY

LEU

10

GLU

ALA

PRO

ARG

PHE

LEU

ALA

11

PHE

LEU

ALA

GLU

LEU

ALA

TYR

ARG

LEU

ALA

12

SER

PRO

GLU

ALA

PRO

ARG

ILE

TYR

PRO

13

LEU

VAL

SER

GLY

LEU

ARG

GLY

ILE

ALA

14

LYS

HIS

GLU

ASP

PRO

LEU

PRO

LEU

VAL

15

TRP

ALA

GLY

TRP

ARG

VAL

ALA

LYS

ALA

GLY

16

GLY

ILE

GLY

PRO

ASN

LEU

GLU

GLY

GLU

GLY

17

LEU

ARG

LEU

LYS

ARG

GLY

ARG

LEU

GLY

GLU

18

GLU

GLY

VAL

TYR

LEU

GLY

ARG

GLU

GLY

VAL

19

GLU

ALA

LEU

LYS

ALA

THR

LEU

ARG

LEU

PRO

20

GLY

ALA

GLN

ALA

LEU

PRO

HIS

LEU

GLU

GLY

21

ALA

PRO

LEU

ASN

ARG

LEU

PHE

LEU

ALA

LEU

22

LYS

ALA

HIS

ALA

GLU

ALA

LEU

GLY

PRO

23

LEU

ARG

SER

ALA

GLU

ALA

ILE

GLY

VAL

Samples:

sample_1: RecO, [U-100% 13C; U-100% 15N], 0.4 mM; TRIS 20 mM; DTT 2 mM; EDTA 2 mM; Magnesium chloride 10 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 7.5; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	11047
DBJ	BAD70446
GB	AAS80606 AEG33049 AFH39328 EIA39541
REF	WP_008632002 WP_011228077 WP_014510042 WP_014629934 WP_024119227