BMRB Entry 11047

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11047

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Title:
RecO-bound ssDNA
Deposition date:
2008-05-15
Original release date:
2009-10-15
Authors:
Shibata, Takehiko; Ito, Yutaka
Citation:

Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems."  J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448

Assembly members:

Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
ttRecO, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA (5'-D(*DTP*DAP*DCP*DG)-3'): TACG
ttRecO: MAPAYTGKVERYRLEEGIVV GRKPLPQGDLLLRLVTPRGS LEAVVRKGQRPTGRTGRLSL FHHVRFQLYAKGEGLPTLTQ AELLGRLHGLEAPRRFLLAA FLAELAYRLASPEAAPRIYP LLVSGLRGIAKHEDPLLPLV WAGWRVAKAGGIGPNLEGEG LRLKRGRLGEEGVYLGREGV EALKATLRLPGAQALPHLEG APLNRLFLALKAHAEEALGP LRSAEAIGV

Data sets:
Data typeCount
1H chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*DTP*DAP*DCP*DG)-3') 11
2DNA (5'-D(*DTP*DAP*DCP*DG)-3') 21
3DNA (5'-D(*DTP*DAP*DCP*DG)-3') 31
4DNA (5'-D(*DTP*DAP*DCP*DG)-3') 41
5DNA (5'-D(*DTP*DAP*DCP*DG)-3') 51
6DNA (5'-D(*DTP*DAP*DCP*DG)-3') 61
7DNA (5'-D(*DTP*DAP*DCP*DG)-3') 71
8DNA (5'-D(*DTP*DAP*DCP*DG)-3') 81
9DNA (5'-D(*DTP*DAP*DCP*DG)-3') 91
10DNA (5'-D(*DTP*DAP*DCP*DG)-3') 101
11ttRecO2

Entities:

Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.

1   DTDADCDG

Entity 2, ttRecO 229 residues - Formula weight is not available

1   METALAPROALATYRTHRGLYLYSVALGLU
2   ARGTYRARGLEUGLUGLUGLYILEVALVAL
3   GLYARGLYSPROLEUPROGLNGLYASPLEU
4   LEULEUARGLEUVALTHRPROARGGLYSER
5   LEUGLUALAVALVALARGLYSGLYGLNARG
6   PROTHRGLYARGTHRGLYARGLEUSERLEU
7   PHEHISHISVALARGPHEGLNLEUTYRALA
8   LYSGLYGLUGLYLEUPROTHRLEUTHRGLN
9   ALAGLULEULEUGLYARGLEUHISGLYLEU
10   GLUALAPROARGARGPHELEULEUALAALA
11   PHELEUALAGLULEUALATYRARGLEUALA
12   SERPROGLUALAALAPROARGILETYRPRO
13   LEULEUVALSERGLYLEUARGGLYILEALA
14   LYSHISGLUASPPROLEULEUPROLEUVAL
15   TRPALAGLYTRPARGVALALALYSALAGLY
16   GLYILEGLYPROASNLEUGLUGLYGLUGLY
17   LEUARGLEULYSARGGLYARGLEUGLYGLU
18   GLUGLYVALTYRLEUGLYARGGLUGLYVAL
19   GLUALALEULYSALATHRLEUARGLEUPRO
20   GLYALAGLNALALEUPROHISLEUGLUGLY
21   ALAPROLEUASNARGLEUPHELEUALALEU
22   LYSALAHISALAGLUGLUALALEUGLYPRO
23   LEUARGSERALAGLUALAILEGLYVAL

Samples:

sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 0.5 mM; ttRecO 0.05 mM; MgCl2 5 mM; NaCl 150 mM; D2O 100%

sample_conditions_1: ionic strength: 150 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
transferred NOESYsample_1isotropicsample_conditions_1
DQF-COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1
ROESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11177
DBJ BAD70446
GB AAS80606 AEG33049 AFH39328 EIA39541
REF WP_008632002 WP_011228077 WP_014510042 WP_014629934 WP_024119227