BMRB Entry 7231

Title:
Solution structure of the heme-binding protein p22HBP
Deposition date:
2006-07-14
Original release date:
2006-10-19
Authors:
Gell, D.; Mackay, J.
Citation:

Citation: Gell, D.; Westmann, B.; Gorman, D.; Liew, C.; Welch, J.; Weiss, M.; Mackay, J.. "A novel haem-binding interface in the 22 kDa haem-binding protein p22HBP"  J. Mol. Biol. 362, 287-297 (2006).
PubMed: 16905148

Assembly members:

Assembly members:
22 kDa heme binding protein, polymer, 192 residues, 21197 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T2

Data sets:
Data typeCount
13C chemical shifts565
15N chemical shifts172
1H chemical shifts1082
coupling constants53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Heme-binding protein 1, p22HBP1

Entities:

Entity 1, Heme-binding protein 1, p22HBP 192 residues - 21197 Da.

1   GLYSERMETLEUGLYMETILEARGASNSER
2   LEUPHEGLYSERVALGLUTHRTRPPROTRP
3   GLNVALLEUSERTHRGLYGLYLYSGLUASP
4   VALSERTYRGLUGLUARGALACYSGLUGLY
5   GLYLYSPHEALATHRVALGLUVALTHRASP
6   LYSPROVALASPGLUALALEUARGGLUALA
7   METPROLYSILEMETLYSTYRVALGLYGLY
8   THRASNASPLYSGLYVALGLYMETGLYMET
9   THRVALPROVALSERPHEALAVALPHEPRO
10   ASNGLUASPGLYSERLEUGLNLYSLYSLEU
11   LYSVALTRPPHEARGILEPROASNGLNPHE
12   GLNGLYSERPROPROALAPROSERASPGLU
13   SERVALLYSILEGLUGLUARGGLUGLYILE
14   THRVALTYRSERTHRGLNPHEGLYGLYTYR
15   ALALYSGLUALAASPTYRVALALAHISALA
16   THRGLNLEUARGTHRTHRLEUGLUGLYTHR
17   PROALATHRTYRGLNGLYASPVALTYRTYR
18   CYSALAGLYTYRASPPROPROMETLYSPRO
19   TYRGLYARGARGASNGLUVALTRPLEUVAL
20   LYSALA

Samples:

sample_1: 22 kDa heme binding protein, [U-95% 13C; U-95% 15N], 0.5 – 1.0 mM; sodium phosphate (Ha2HPO4/NaH2PO4) 20 mM; 5,5-dimethylsilapentanesulfonate 0.002 mM; D2O 5%

sample_2: 22 kDa heme binding protein, [U-95% 13C; U-95% 15N], 0.5 – 1.0 mM; sodium phosphate (Ha2HPO4/NaH2PO4) 20 mM; 5,5-dimethylsilapentanesulfonate 0.002 mM; D2O%

sample_cond_1: ionic strength: 20 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNHAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

xwinnmr v3.5 patchlevel 6 - collection

SPARKY v3.110 - data analysis

DYANA v5.1 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 17953 6620
PDB
DBJ BAA33770
EMBL CAJ18470
GB AAD32096 AAH12654 AAI68221 EDL10544 EDM01631
REF NP_001102121 NP_038574 XP_006237591
SP Q9R257
AlphaFold Q9R257

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks