BMRB Entry 17953

Name: NMR Structure of protoporphyrin-IX bound murine p22HBP
Published: 2012-09-24

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PDB ID: 4a1m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17953
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structure of protoporphyrin-IX bound murine p22HBP

Deposition date:

2011-09-22

Original release date:

2012-09-24

Authors:

Goodfellow, Brian; Dias, J.; Macedo, A.; Ferreira, G.; Peterson, F.; Volkman, B.; Duarte, I.

Citation:

Citation: Goodfellow, Brian; Dias, J.; Macedo, A.; Ferreira, G.; Peterson, F.; Volkman, B.; Duarte, I.. "The NMR Structure of protoporphyrin-IX bound murine p22HBP" to be published ., .-..

Assembly members:

Assembly members:
HEME-BINDING_PROTEIN_1, polymer, 195 residues, 21737.3322 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PJN2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HEME-BINDING_PROTEIN_1: MKNSTHHHHHHNSLFGSVET WPWQVLSTGGKEDVSYEERA CEGGKFATVEVTDKPVDEAL REAMPKIMKYVGGTNDKGVG MGMTVPVSFAVFPNEDGSLQ KKLKVWFRIPNQFQGSPPAP SDESVKIEEREGITVYSTQF GGYAKEADYVAHATQLRTTL EGTPATYQGDVYYCAGYDPP MKPYGRRNEVWLVKA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	639
15N chemical shifts	166
1H chemical shifts	1031

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HEME-BINDING PROTEIN 1	1

Entities:

Entity 1, HEME-BINDING PROTEIN 1 195 residues - 21737.3322 Da.

1

MET

LYS

ASN

SER

THR

HIS

2

HIS

ASN

SER

LEU

PHE

GLY

SER

VAL

GLU

THR

3

TRP

PRO

TRP

GLN

VAL

LEU

SER

THR

GLY

4

LYS

GLU

ASP

VAL

SER

TYR

GLU

ARG

ALA

5

CYS

GLU

GLY

LYS

PHE

ALA

THR

VAL

GLU

6

VAL

THR

ASP

LYS

PRO

VAL

ASP

GLU

ALA

LEU

7

ARG

GLU

ALA

MET

PRO

LYS

ILE

MET

LYS

TYR

8

VAL

GLY

THR

ASN

ASP

LYS

GLY

VAL

GLY

9

MET

GLY

MET

THR

VAL

PRO

VAL

SER

PHE

ALA

10

VAL

PHE

PRO

ASN

GLU

ASP

GLY

SER

LEU

GLN

11

LYS

LEU

LYS

VAL

TRP

PHE

ARG

ILE

PRO

12

ASN

GLN

PHE

GLN

GLY

SER

PRO

ALA

PRO

13

SER

ASP

GLU

SER

VAL

LYS

ILE

GLU

ARG

14

GLU

GLY

ILE

THR

VAL

TYR

SER

THR

GLN

PHE

15

GLY

TYR

ALA

LYS

GLU

ALA

ASP

TYR

VAL

16

ALA

HIS

ALA

THR

GLN

LEU

ARG

THR

LEU

17

GLU

GLY

THR

PRO

ALA

THR

TYR

GLN

GLY

ASP

18

VAL

TYR

CYS

ALA

GLY

TYR

ASP

PRO

19

MET

LYS

PRO

TYR

GLY

ARG

ASN

GLU

VAL

20

TRP

LEU

VAL

LYS

ALA

Samples:

sample_1: HEME-BINDING_PROTEIN_1, [U-13C; U-15N], 1 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 8.000; pressure: 1.000 atm; temperature: 303.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D/3D 15N NOESY	sample_1	solution	sample_conditions_1
2D/3D 13C NOESY (aliphatic and aromatic)	sample_1	solution	sample_conditions_1

Software:

AutoDep v4.3, AutoDep -

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

Bruker DMX 600 MHz

UNP	HEBP1_MOUSE
PDB	2GOV 2HVA 4A1M
DBJ	BAA33770
EMBL	CAJ18470
GB	AAD32096 AAH12654 AAI68221 EDL10544 EDM01631
REF	NP_038574 XP_006237591 XP_011239526
SP	Q9R257
AlphaFold	O88814 Q9R257