BMRB Entry 7140

Title:
Csk SH2 domain, free form
Deposition date:
2006-06-02
Original release date:
2011-05-26
Authors:
Liu, Dongsheng; Cowburn, David
Citation:

Citation: Cowburn, David; Liu, Dongsheng. "Domain interactions in protein tyrosine kinase Csk identified by segmental labeling NMR methods"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
SH2, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts92
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2 domain of human Csk1

Entities:

Entity 1, SH2 domain of human Csk 106 residues - Formula weight is not available

1   ALAGLYTHRLYSLEUSERLEUMETPROTRP
2   PHEHISGLYLYSILETHRARGGLUGLNALA
3   GLUARGLEULEUTYRPROPROGLUTHRGLY
4   LEUPHELEUVALARGGLUSERTHRASNTYR
5   PROGLYASPTYRTHRLEUCYSVALSERCYS
6   ASPGLYLYSVALGLUHISTYRARGILEMET
7   TYRHISALASERLYSLEUSERILEASPGLU
8   GLUVALTYRPHEGLUASNLEUMETGLNLEU
9   VALGLUHISTYRTHRSERASPALAASPGLY
10   LEUCYSTHRARGLEUILELYSPROLYSVAL
11   METGLUGLYTHRVALALA

Samples:

sample_1: SH2, [U-95% 13C; U-90% 15N], 0.8 ± 0.1 mM

conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC_(example)sample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 7141
PDB
DBJ BAD97346 BAE23297 BAE33565 BAE42047 BAF83220
EMBL CAA41484 CAA42556 CAA42713 CAB58562 CAG46758
GB AAA18766 AAH18394 AAH52006 AAH98863 AAI04848
PRF 1709363A
REF NP_001025210 NP_001068865 NP_001120662 NP_001177102 NP_001248565
SP P32577 P41240 P41241 Q0VBZ0
TPG DAA17512
AlphaFold P32577 Q0VBZ0 P41241 P41240

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks