BMRB Entry 6918

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for CRC-N (algal centrin N-term)
Deposition date:
2005-12-06
Original release date:
2007-01-29
Authors:
Sheehan, Jonathan; Bunick, Christopher; Hu, Haitao; Fagan, Patricia; Meyn, Susan; Chazin, Walter
Citation:

Citation: Sheehan, Jonathan; Bunick, Christopher; Hu, Haitao; Fagan, Patricia; Meyn, Susan; Chazin, Walter. "Structure of the N-terminal Calcium Sensor Domain of Centrin Reveals the Biochemical Basis for Domain-Specific Function"  J. Biol. Chem. 281, 2876-2881 (2006).
PubMed: 16317001

Assembly members:

Assembly members:
CRC-N, polymer, 96 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: green algae   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts93
1H chemical shifts659

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain of caltractin1
2CALCIUM (II) ION2
3CALCIUM (II) ION2

Entities:

Entity 1, N-terminal domain of caltractin 96 residues - Formula weight is not available

1   GLYSERMETSERTYRLYSALALYSTHRVAL
2   VALSERALAARGARGASPGLNLYSLYSGLY
3   ARGVALGLYLEUTHRGLUGLUGLNLYSGLN
4   GLUILEARGGLUALAPHEASPLEUPHEASP
5   THRASPGLYSERGLYTHRILEASPALALYS
6   GLULEULYSVALALAMETARGALALEUGLY
7   PHEGLUPROLYSLYSGLUGLUILELYSLYS
8   METILESERGLUILEASPLYSASPGLYSER
9   GLYTHRILEASPPHEGLUGLUPHELEUTHR
10   METMETTHRALALYSMET

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CRC-N, [U-13C; U-15N], 1 mM; CaCl25 – 10 mM; Tris-d11 25 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
(H)CC(CO)NHsample_1not availableconditions_1
H(CCCO)NHsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
1H COSYsample_1not availableconditions_1
1H 2-quantumsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 6820
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks