BMRB Entry 6832

Title:
Comparative NMR study on the impact of point mutations on protein stability of Pseudomonas mendocina lipase
Deposition date:
2005-09-21
Original release date:
2006-08-07
Authors:
Favier, Adrien; Sibille, Nathalie; van Nuland, Nico; Boelens, Rolf; Ganshaw, Grant; Bott, Rick
Citation:

Citation: Sibille, Nathalie; Favier, Adrien; Azuaga, Ana; Ganshaw, Grant; Bott, Rick; Bonvin, Alexandre; Boelens, Rolf; van Nuland, Nico. "Comparative NMR study on the impact of point mutations on protein stability of Pseudomonas mendocina lipase"  Protein Sci. 15, 1915-1927 (2006).
PubMed: 16823035

Assembly members:

Assembly members:
Pseudomonas mendocina lipase enzyme, polymer, 258 residues, Formula weight is not available
DIETHYL P-NITROPHENYL PHOSPHATE, non-polymer, 275.195 Da.

Natural source:

Natural source:   Common Name: Pseudomonas mendocina   Taxonomy ID: 300   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas mendocina

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts671
15N chemical shifts237
1H chemical shifts237

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lipase PG1
2e6002

Entities:

Entity 1, lipase PG 258 residues - Formula weight is not available

1   ALAPROLEUPROASPTHRPROGLYALAPRO
2   PHEPROALAVALALAASNPHEASPARGSER
3   GLYPROTYRTHRVALSERSERGLNSERGLU
4   GLYPROSERCYSARGILETYRARGPROARG
5   ASPLEUGLYGLNGLYGLYVALARGHISPRO
6   VALILELEUTRPGLYASNGLYTHRGLYALA
7   GLYPROSERTHRTYRALAGLYLEULEUSER
8   HISTRPALASERHISGLYPHEVALVALALA
9   ALAALAGLUTHRSERASNALAGLYTHRGLY
10   ARGGLUMETLEUALACYSLEUASPTYRLEU
11   VALARGGLUASNASPTHRPROTYRGLYTHR
12   TYRSERGLYLYSLEUASNTHRGLYARGVAL
13   GLYTHRSERGLYHISSERGLNGLYGLYGLY
14   GLYSERILEMETALAGLYGLNASPTHRARG
15   VALARGTHRTHRALAPROILEGLNPROTYR
16   THRLEUGLYLEUGLYHISASPSERALASER
17   GLNARGARGGLNGLNGLYPROMETPHELEU
18   METSERGLYGLYGLYASPTHRILEALAPRO
19   PROTYRLEUASNALAGLNPROVALTYRARG
20   ARGALAASNVALPROVALPHETRPGLYGLU
21   ARGARGTYRVALGLYHISPHEGLUPROVAL
22   GLYSERGLYGLYALATYRARGGLYPROSER
23   THRALATRPPHEARGPHEGLNLEUMETASP
24   ASPGLNASPALAARGALATHRPHETYRGLY
25   ALAGLNCYSSERLEUCYSTHRSERLEULEU
26   TRPSERVALGLUARGARGGLYLEU

Entity 2, e600 - C10 H14 N O6 P - 275.195 Da.

1   EPP

Samples:

sample_1: Pseudomonas mendocina lipase enzyme, [U-95% 13C; U-90% 15N], 0.8 ± 0.1 mM

sample_2: Pseudomonas mendocina lipase enzyme, [U-90% 15N], 0.4 ± 0.1 mM

conditions_1: pH: 5.8; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
1H-15N heteronuclear NOEnot availablenot availableconditions_1
15N T1not availablenot availableconditions_1
15N T1 rhonot availablenot availableconditions_1

Software:

xwinnmr v3.5 -

NMR spectrometers:

  • BRUKER advance ULTRA SHIELDED 600 MHz

Related Database Links:

BMRB 6935
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks