BMRB Entry 6935

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6935

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Title:
Comparative NMR study on the impact of point mutations on protein stability of Pseudomonas mendocina lipase
Deposition date:
2006-01-11
Original release date:
2006-08-07
Authors:
Favier, Adrien; Sibille, Nathalie; van Nuland, Nico; Boelens, Rolf; Ganshaw, Grant; Bott, Rick
Citation:

Citation: Sibille, Nathalie; Favier, Adrien; Azuaga, Ana; Ganshaw, Grant; Bott, Rick; Bonvin, Alexandre; Boelens, Rolf; van Nuland, Nico. "Comparative NMR study on the impact of point mutations on protein stability of Pseudomonas mendocina lipase"  Protein Sci. 15, 1915-1927 (2006).
PubMed: 16823035

Assembly members:

Assembly members:
Pseudomonas mendocina lipase enzyme, polymer, 258 residues, Formula weight is not available
EPP, non-polymer, 275.195 Da.

Natural source:

Natural source:   Common Name: Pseudomonas mendocina   Taxonomy ID: 300   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas mendocina

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pseudomonas mendocina lipase enzyme: APLPDTPGAPFPAVANFDRS GPYTVSSQSEGPSCRIYRPR DLGQGGVRHPVILWGNGTGA GPSTYAGLLSHWASHGFVVA AAETSNAGTGREMLACLDYL VRENDTPYGTYSGKLNTGRV GTSGHSQGGGGSIMAGQDTR VRTTAPIQPYTLGLGHDSAS QRRQQGPMFLMSGGGDTIAF PYLNAQPVYRRANVPVFWGE RRYVSHFEPVGSGGAYRGPS TAWFRFQLMDDQDARATFYG AQCSLCTSLLWSVERRGL

Data sets:
Data typeCount
13C chemical shifts435
15N chemical shifts235
1H chemical shifts235

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lipase WT1
2e6002

Entities:

Entity 1, lipase WT 258 residues - Formula weight is not available

1   ALAPROLEUPROASPTHRPROGLYALAPRO
2   PHEPROALAVALALAASNPHEASPARGSER
3   GLYPROTYRTHRVALSERSERGLNSERGLU
4   GLYPROSERCYSARGILETYRARGPROARG
5   ASPLEUGLYGLNGLYGLYVALARGHISPRO
6   VALILELEUTRPGLYASNGLYTHRGLYALA
7   GLYPROSERTHRTYRALAGLYLEULEUSER
8   HISTRPALASERHISGLYPHEVALVALALA
9   ALAALAGLUTHRSERASNALAGLYTHRGLY
10   ARGGLUMETLEUALACYSLEUASPTYRLEU
11   VALARGGLUASNASPTHRPROTYRGLYTHR
12   TYRSERGLYLYSLEUASNTHRGLYARGVAL
13   GLYTHRSERGLYHISSERGLNGLYGLYGLY
14   GLYSERILEMETALAGLYGLNASPTHRARG
15   VALARGTHRTHRALAPROILEGLNPROTYR
16   THRLEUGLYLEUGLYHISASPSERALASER
17   GLNARGARGGLNGLNGLYPROMETPHELEU
18   METSERGLYGLYGLYASPTHRILEALAPHE
19   PROTYRLEUASNALAGLNPROVALTYRARG
20   ARGALAASNVALPROVALPHETRPGLYGLU
21   ARGARGTYRVALSERHISPHEGLUPROVAL
22   GLYSERGLYGLYALATYRARGGLYPROSER
23   THRALATRPPHEARGPHEGLNLEUMETASP
24   ASPGLNASPALAARGALATHRPHETYRGLY
25   ALAGLNCYSSERLEUCYSTHRSERLEULEU
26   TRPSERVALGLUARGARGGLYLEU

Entity 2, e600 - C10 H14 N O6 P - 275.195 Da.

1   EPP

Samples:

sample_1: Pseudomonas mendocina lipase enzyme, [U-95% 13C; U-90% 15N], 0.8 ± 0.1 mM

sample_2: Pseudomonas mendocina lipase enzyme, [U-90% 15N], 0.4 ± 0.1 mM

conditions_1: pH: 5.8; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 6832
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks