BMRB Entry 6801

Title:
1H, 13C, and 15N Chemical Shift Assignments for Human Small Ubiquitin-like Modifier Protein Isoform 2 (SUMO-2)
Deposition date:
2005-08-30
Original release date:
2007-04-11
Authors:
Chang, Chung-ke; Wang, Ying Hui; Chung, Tung-Liang; Chang, Chi-Fon; Li, Steven; Huang, Tai-huang
Citation:

Citation: Chang, Chung-ke; Wang, Ying Hui; Chang, Chi-Fon; Chung, Tung-Liang; Tien, Shi-chi; Li, Steven; Huang, Tai-huang. "Solution structure and dynamics of human SUMO-2"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
human SUMO-2, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts366
15N chemical shifts92
1H chemical shifts584

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human SUMO-2 monomer1

Entities:

Entity 1, Human SUMO-2 monomer 96 residues - Formula weight is not available

1   METALAASPGLULYSPROLYSGLUGLYVAL
2   LYSTHRGLUASNASNASPHISILEASNLEU
3   LYSVALALAGLYGLNASPGLYSERVALVAL
4   GLNPHELYSILELYSARGHISTHRPROLEU
5   SERLYSLEUMETLYSALATYRCYSGLUARG
6   GLNGLYLEUSERMETARGGLNILEARGPHE
7   ARGPHEASPGLYGLNPROILEASNGLUTHR
8   ASPTHRPROALAGLNLEUGLUMETGLUASP
9   GLUASPTHRILEASPVALPHEGLNGLNGLN
10   THRGLYGLYVALTYRTYR

Samples:

sample_1: human SUMO-2, [U-95% 13C; U-95% 15N], 0.8 ± 0.1 mM; sodium phoshate 50 ± 1 mM; sodium chloride 150 ± 1 mM; EDTA 1 ± 0.01 mM; DSS 1 ± 0.01 mM; deuterium oxide 10 ± 1 %; water 90 ± 1 %; sodium azide 0.01 ± 0.0001 %; Complete(R) protease inhibitor cocktail

conditions_1: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H15N_NOESY_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HCCH_TOCSYsample_1not availableconditions_1
1H13C_NOESY_HSQCsample_1not availableconditions_1
HBCB(CGCD)HDsample_1not availableconditions_1
HBCB(CGCDCE)HEsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 11267 19961
PDB
DBJ BAB28360 BAC39397 BAE21037 BAE35772 BAE39412
EMBL CAA67897 CAG32064 CAL37097
GB AAB49682 AAB92355 AAD45399 AAH08450 AAH16775
REF NP_001003422 NP_001074186 NP_001165519 NP_001185620 NP_001231073
SP P61955 P61956 P61957 P61958 P61959
TPG DAA13334 DAA18189
AlphaFold P61957 P61955 P61956 P61958 P61959

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks