BMRB Entry 6602

Title:
1H, 13C, and 15N Chemical Shift Assignments for the GAF A Domain of Phosphodiesterase 5
Deposition date:
2005-04-20
Original release date:
2005-10-27
Authors:
Sekharan, Monica; Rajagopal, Ponni; Klevit, Rachel
Citation:

Citation: Sekharan, Monica; Rajagopal, Ponni; Klevit, Rachel. "Backbone 1H, 13C, and 15N Resonance Assignment of the 46 kDa Dimeric GAF A Domain of Phosphodiesterase 5"  J. Biomol. NMR 33, 75-75 (2005).
PubMed: 16222566

Assembly members:

Assembly members:
5 GAF A, polymer, 204 residues, Formula weight is not available
35G, non-polymer, 345.205 Da.

Natural source:

Natural source:   Common Name: House Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDE5 GAF A domain1
2cGMP1

Entities:

Entity 1, PDE5 GAF A domain 204 residues - Formula weight is not available

1   PROLEUTHRPROPROARGPHEASPSERASP
2   GLUVALASPGLNCYSSERARGLEULEUGLU
3   LEUVALLYSASPILESERSERHISLEUASP
4   VALTHRALALEUCYSHISLYSILEPHELEU
5   HISILEHISGLYLEUILESERALAASPARG
6   TYRSERLEUPHELEUVALCYSGLUASPSER
7   SERLYSASPLYSPHELEUILESERARGLEU
8   PHEASPVALALAGLUGLYSERTHRLEUGLU
9   GLUALASERASNASNCYSILEARGLEUGLU
10   TRPASNLYSGLYILEVALGLYHISVALALA
11   ALAPHEGLYGLUPROLEUASNILELYSASP
12   ALATYRGLUASPPROARGPHEASNALAGLU
13   VALASPGLNILETHRGLYTYRLYSTHRGLN
14   SERILELEUCYSMETPROILELYSASNHIS
15   ARGGLUGLUVALVALGLYVALALAGLNALA
16   ILEASNLYSLYSSERGLYASNGLYGLYTHR
17   PHETHRGLULYSASPGLULYSASPPHEALA
18   ALATYRLEUALAPHECYSGLYILEVALLEU
19   HISASNALAGLNLEUTYRGLUTHRSERLEU
20   LEUGLUASNLYSARGASNLEUGLUHISHIS
21   HISHISHISHIS

Samples:

15N_cGMP-bound_PDE5_GAF_A_NMR_sample: PDE5 GAF A, [U-98% 15N], 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

13C-15N_cGMP-bound_PDE5_GAF_A_NMR_sample: PDE5 GAF A, [U-99% 13C; U-99% 15N], 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

2H-13C-15N_cGMP-bound_PDE5_GAF_A_NMR_sample: PDE5 GAF A, [U-99% 2H; U-99% 13C; U-98% 15N], 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Ala: PDE5 GAF A, [U-98% 15N]-Ala, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Ile: PDE5 GAF A, [U-98% 15N]-Ile, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Leu: PDE5 GAF A, [U-98% 15N]-Leu, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Val: PDE5 GAF A, [U-98% 15N]-Val, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Asp: PDE5 GAF A, [U-98% 15N]-Asp, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Glu: PDE5 GAF A, [U-98% 15N]-Glu, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Phe: PDE5 GAF A, [U-98% 15N]-Phe, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Lys: PDE5 GAF A, [U-98% 15N]-Lys, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

Sample_condition_set_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableSample_condition_set_1
HN(CO)CAnot availablenot availableSample_condition_set_1
HNCACBnot availablenot availableSample_condition_set_1
HN(CO)CACBnot availablenot availableSample_condition_set_1
HNCOnot availablenot availableSample_condition_set_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz MHz

Related Database Links:

BMRB 15734
PDB
DBJ BAA84659 BAA84660 BAC27332
GB AAP31235

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks