BMRB Entry 15734
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PDB ID: 2k31
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15734
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Heikaus, Clemens; Stout, Joseph; Sekharan, Monica; Eakin, Catherine; Rajagopal, Ponni; Brzovic, Peter; Beavo, Joseph; Klevit, Rachel. "Solution Structure of the cGMP Binding GAF Domain from Phosphodiesterase 5: INSIGHTS INTO NUCLEOTIDE SPECIFICITY, DIMERIZATION, AND cGMP-DEPENDENT CONFORMATIONAL CHANGE" J. Biol. Chem. 283, 22749-22759 (2008).
PubMed: 18534985
Assembly members:
mPDE5_GAFA, polymer, 176 residues, 20037.4 Da.
GUANOSINE-3',5'-MONOPHOSPHATE, non-polymer, 345.205 Da.
Natural source: Common Name: Mus musculus Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRunH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 978 |
| 13C chemical shifts | 636 |
| 15N chemical shifts | 151 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | GUANOSINE-3',5'-MONOPHOSPHATE | 2 |
Entities:
Entity 1, entity_1 176 residues - 20037.4 Da.
| 1 | MET | ASP | VAL | THR | ALA | LEU | CYS | HIS | LYS | ILE | ||||
| 2 | PHE | LEU | HIS | ILE | HIS | GLY | LEU | ILE | SER | ALA | ||||
| 3 | ASP | ARG | TYR | SER | LEU | PHE | LEU | VAL | CYS | GLU | ||||
| 4 | ASP | SER | SER | LYS | ASP | LYS | PHE | LEU | ILE | SER | ||||
| 5 | ARG | LEU | PHE | ASP | VAL | ALA | GLU | GLY | SER | THR | ||||
| 6 | LEU | GLU | GLU | ALA | SER | ASN | ASN | CYS | ILE | ARG | ||||
| 7 | LEU | GLU | TRP | ASN | LYS | GLY | ILE | VAL | GLY | HIS | ||||
| 8 | VAL | ALA | ALA | PHE | GLY | GLU | PRO | LEU | ASN | ILE | ||||
| 9 | LYS | ASP | ALA | TYR | GLU | ASP | PRO | ARG | PHE | ASN | ||||
| 10 | ALA | GLU | VAL | ASP | GLN | ILE | THR | GLY | TYR | LYS | ||||
| 11 | THR | GLN | SER | ILE | LEU | CYS | MET | PRO | ILE | LYS | ||||
| 12 | ASN | HIS | ARG | GLU | GLU | VAL | VAL | GLY | VAL | ALA | ||||
| 13 | GLN | ALA | ILE | ASN | LYS | LYS | SER | GLY | ASN | GLY | ||||
| 14 | GLY | THR | PHE | THR | GLU | LYS | ASP | GLU | LYS | ASP | ||||
| 15 | PHE | ALA | GLU | TYR | LEU | ALA | PHE | CYS | GLY | GLU | ||||
| 16 | VAL | LEU | HIS | ASN | ALA | GLN | LEU | TYR | GLU | THR | ||||
| 17 | SER | LEU | LEU | GLU | ASN | LYS | ARG | ASN | LEU | GLU | ||||
| 18 | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, GUANOSINE-3',5'-MONOPHOSPHATE - C10 H12 N5 O7 P - 345.205 Da.
| 1 | 35G |
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