BMRB Entry 6385

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for pleckstrin human DEP domain
Deposition date:
2004-11-10
Original release date:
2005-01-14
Authors:
Civera, Concepcion; Simon, Bernd; Stier, Gunter; Sattler, Michael; Macias, Maria
Citation:

Citation: Civera, Concepcion; Simon, Bernd; Stier, Gunter; Sattler, Michael; Macias, Maria. "Structure and dynamics of the human pleckstrin DEP domain: distinct molecular features of a novel DEP domain subfamily."  Proteins 58, 354-366 (2005).
PubMed: 15573383

Assembly members:

Assembly members:
pleckstrin, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1087
13C chemical shifts328
15N chemical shifts112
coupling constants71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DEP monomer1

Entities:

Entity 1, DEP monomer 103 residues - Formula weight is not available

1   METGLUTHRILEASPLEUGLYALALEUTYR
2   LEUSERMETLYSASPTHRGLULYSGLYILE
3   LYSGLULEUASNLEUGLULYSASPLYSLYS
4   ILEPHEASNHISCYSPHETHRGLYASNCYS
5   VALILEASPTRPLEUVALSERASNGLNSER
6   VALARGASNARGGLNGLUGLYLEUMETILE
7   ALASERSERLEULEUASNGLUGLYTYRLEU
8   GLNPROALAGLYASPMETSERLYSSERALA
9   VALASPGLYTHRALAGLUASNPROPHELEU
10   ASPASNPROASPALAPHETYRTYRPHEPRO
11   ASPSERGLY

Samples:

sample_1: pleckstrin, [U-13C; U-15N], 0.5 – 1.5 mM

Ex-cond_1: pH: 6.5; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
1H-15N HSQCsample_1not availableEx-cond_1
CBCANHsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
H(CC)(CO)NHsample_1not availableEx-cond_1
C(CO)NHsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
HBCBCCDHDsample_1not availableEx-cond_1
HBCBCCDCEHEsample_1not availableEx-cond_1
2D TOCSYsample_1not availableEx-cond_1
2DNOESYsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • BRUKER DRX 500 MHz
  • BRUKER DRX 600 MHz

Related Database Links:

BMRB 6873
PDB
DBJ BAD92204 BAG36495 BAI47103
EMBL CAA30564 CAG46853 CAG46876 CAH04268
GB AAH18549 AAP36299 AAX37008 AAX43402 AAX93121
PRF 1408254A
REF NP_002655 XP_001094492 XP_002811995 XP_003262529 XP_003830964
SP P08567
AlphaFold P08567