BMRB Entry 6873

Name: 1H, 15N and 13C chemical shift assignments of pleckstrin&#039;s C-terminal PH domain
Published: 2006-05-02

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6873

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C chemical shift assignments of pleckstrin's C-terminal PH domain

Deposition date:

2005-10-25

Original release date:

2006-05-02

Authors:

Edlich, Christian; Simon, Bernd; Stier, Gunter; Sattler, Michael; Muhle-Goll, Claudia

Citation:

Citation: Edlich, Christian; Simon, Bernd; Sattler, Michael; Muhle-Goll, Claudia. "New NMR Assignment 1H, 13C, and 15N Assignment of the Second PH Domain of Human Pleckstrin (234-350)" J. Biomol. NMR 36, 21-21 (2006).
PubMed: 16642402

Assembly members:

Assembly members:
C-PH, polymer, 360 residues, 13455.3 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-PH: MHHHHHHKPMMEPKRIREGY LVKKGSVFNTWKPMWVVLLE DGIEFYKKKSDNSPKGMIPL KGSTLTSPCQDFGKRMFVFK ITTTKQQDHFFQAAFLEERD AWVRDINKAIKCIEGGQKFA RKSTRRSIRLPETIDLGALY LSMKDTEKGIKELNLEKDKK IFNHCFTGNCVIDWLVSNQS VRNRQEGLMIASSLLNEGYL QPAGDMSKSAVDGTAENPFL DNPDAFYYFPDSGFFCEENS SDDDVILKEEFRGVIIKQGC LLKQGHRRKNWKVRKFILRE DPAYLHYYDPAGAEDPLGAI HLRGCVVTSVESNSNGRKSE EENLFEIITADEVHYFLQAA TPKERTEWIKAIQMASRTGK

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	401
15N chemical shifts	120
1H chemical shifts	790

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pleckstrin C-PH	1

Entities:

Entity 1, pleckstrin C-PH 360 residues - 13455.3 Da.

assignment starting from residue 234 to 350.

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	LYS	PRO	MET
2	MET	GLU	PRO	LYS	ARG	ILE	ARG	GLU	GLY	TYR
3	LEU	VAL	LYS	LYS	GLY	SER	VAL	PHE	ASN	THR
4	TRP	LYS	PRO	MET	TRP	VAL	VAL	LEU	LEU	GLU
5	ASP	GLY	ILE	GLU	PHE	TYR	LYS	LYS	LYS	SER
6	ASP	ASN	SER	PRO	LYS	GLY	MET	ILE	PRO	LEU
7	LYS	GLY	SER	THR	LEU	THR	SER	PRO	CYS	GLN
8	ASP	PHE	GLY	LYS	ARG	MET	PHE	VAL	PHE	LYS
9	ILE	THR	THR	THR	LYS	GLN	GLN	ASP	HIS	PHE
10	PHE	GLN	ALA	ALA	PHE	LEU	GLU	GLU	ARG	ASP
11	ALA	TRP	VAL	ARG	ASP	ILE	ASN	LYS	ALA	ILE
12	LYS	CYS	ILE	GLU	GLY	GLY	GLN	LYS	PHE	ALA
13	ARG	LYS	SER	THR	ARG	ARG	SER	ILE	ARG	LEU
14	PRO	GLU	THR	ILE	ASP	LEU	GLY	ALA	LEU	TYR
15	LEU	SER	MET	LYS	ASP	THR	GLU	LYS	GLY	ILE
16	LYS	GLU	LEU	ASN	LEU	GLU	LYS	ASP	LYS	LYS
17	ILE	PHE	ASN	HIS	CYS	PHE	THR	GLY	ASN	CYS
18	VAL	ILE	ASP	TRP	LEU	VAL	SER	ASN	GLN	SER
19	VAL	ARG	ASN	ARG	GLN	GLU	GLY	LEU	MET	ILE
20	ALA	SER	SER	LEU	LEU	ASN	GLU	GLY	TYR	LEU
21	GLN	PRO	ALA	GLY	ASP	MET	SER	LYS	SER	ALA
22	VAL	ASP	GLY	THR	ALA	GLU	ASN	PRO	PHE	LEU
23	ASP	ASN	PRO	ASP	ALA	PHE	TYR	TYR	PHE	PRO
24	ASP	SER	GLY	PHE	PHE	CYS	GLU	GLU	ASN	SER
25	SER	ASP	ASP	ASP	VAL	ILE	LEU	LYS	GLU	GLU
26	PHE	ARG	GLY	VAL	ILE	ILE	LYS	GLN	GLY	CYS
27	LEU	LEU	LYS	GLN	GLY	HIS	ARG	ARG	LYS	ASN
28	TRP	LYS	VAL	ARG	LYS	PHE	ILE	LEU	ARG	GLU
29	ASP	PRO	ALA	TYR	LEU	HIS	TYR	TYR	ASP	PRO
30	ALA	GLY	ALA	GLU	ASP	PRO	LEU	GLY	ALA	ILE
31	HIS	LEU	ARG	GLY	CYS	VAL	VAL	THR	SER	VAL
32	GLU	SER	ASN	SER	ASN	GLY	ARG	LYS	SER	GLU
33	GLU	GLU	ASN	LEU	PHE	GLU	ILE	ILE	THR	ALA
34	ASP	GLU	VAL	HIS	TYR	PHE	LEU	GLN	ALA	ALA
35	THR	PRO	LYS	GLU	ARG	THR	GLU	TRP	ILE	LYS
36	ALA	ILE	GLN	MET	ALA	SER	ARG	THR	GLY	LYS

Samples:

sample_1: Pleckstrin, [U-2H; U-13C; U-15N], 0.4 mM; sodium phosphate buffer 20 mM; NaCl 100 mM; DTT 2 mM; sodium azide 0.02%

conditions_1: ionic strength: 100 mM; pH: 6.8; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	conditions_1
1H13C HSQC	sample_1	not available	conditions_1
Triple-resonance experiments	sample_1	not available	conditions_1
HCCH tocsy	sample_1	not available	conditions_1
13C-HMQC-NOESY	sample_1	not available	conditions_1
15N-HSQC-NOESY	sample_1	not available	conditions_1

Software:

NMRView -

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 500 MHz

DBJ	BAG36495 BAI47103
EMBL	CAA30564 CAG46853 CAG46876
GB	AAH18549 AAP36299 AAX37008 AAX43402 AAX93121
PRF	1408254A
REF	NP_002655 XP_001094492 XP_002811995 XP_003262529 XP_003830964
SP	P08567
AlphaFold	P08567

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	LYS	PRO	MET
2	MET	GLU	PRO	LYS	ARG	ILE	ARG	GLU	GLY	TYR
3	LEU	VAL	LYS	LYS	GLY	SER	VAL	PHE	ASN	THR
4	TRP	LYS	PRO	MET	TRP	VAL	VAL	LEU	LEU	GLU
5	ASP	GLY	ILE	GLU	PHE	TYR	LYS	LYS	LYS	SER
6	ASP	ASN	SER	PRO	LYS	GLY	MET	ILE	PRO	LEU
7	LYS	GLY	SER	THR	LEU	THR	SER	PRO	CYS	GLN
8	ASP	PHE	GLY	LYS	ARG	MET	PHE	VAL	PHE	LYS
9	ILE	THR	THR	THR	LYS	GLN	GLN	ASP	HIS	PHE
10	PHE	GLN	ALA	ALA	PHE	LEU	GLU	GLU	ARG	ASP
11	ALA	TRP	VAL	ARG	ASP	ILE	ASN	LYS	ALA	ILE
12	LYS	CYS	ILE	GLU	GLY	GLY	GLN	LYS	PHE	ALA
13	ARG	LYS	SER	THR	ARG	ARG	SER	ILE	ARG	LEU
14	PRO	GLU	THR	ILE	ASP	LEU	GLY	ALA	LEU	TYR
15	LEU	SER	MET	LYS	ASP	THR	GLU	LYS	GLY	ILE
16	LYS	GLU	LEU	ASN	LEU	GLU	LYS	ASP	LYS	LYS
17	ILE	PHE	ASN	HIS	CYS	PHE	THR	GLY	ASN	CYS
18	VAL	ILE	ASP	TRP	LEU	VAL	SER	ASN	GLN	SER
19	VAL	ARG	ASN	ARG	GLN	GLU	GLY	LEU	MET	ILE
20	ALA	SER	SER	LEU	LEU	ASN	GLU	GLY	TYR	LEU
21	GLN	PRO	ALA	GLY	ASP	MET	SER	LYS	SER	ALA
22	VAL	ASP	GLY	THR	ALA	GLU	ASN	PRO	PHE	LEU
23	ASP	ASN	PRO	ASP	ALA	PHE	TYR	TYR	PHE	PRO
24	ASP	SER	GLY	PHE	PHE	CYS	GLU	GLU	ASN	SER
25	SER	ASP	ASP	ASP	VAL	ILE	LEU	LYS	GLU	GLU
26	PHE	ARG	GLY	VAL	ILE	ILE	LYS	GLN	GLY	CYS
27	LEU	LEU	LYS	GLN	GLY	HIS	ARG	ARG	LYS	ASN
28	TRP	LYS	VAL	ARG	LYS	PHE	ILE	LEU	ARG	GLU
29	ASP	PRO	ALA	TYR	LEU	HIS	TYR	TYR	ASP	PRO
30	ALA	GLY	ALA	GLU	ASP	PRO	LEU	GLY	ALA	ILE
31	HIS	LEU	ARG	GLY	CYS	VAL	VAL	THR	SER	VAL
32	GLU	SER	ASN	SER	ASN	GLY	ARG	LYS	SER	GLU
33	GLU	GLU	ASN	LEU	PHE	GLU	ILE	ILE	THR	ALA
34	ASP	GLU	VAL	HIS	TYR	PHE	LEU	GLN	ALA	ALA
35	THR	PRO	LYS	GLU	ARG	THR	GLU	TRP	ILE	LYS
36	ALA	ILE	GLN	MET	ALA	SER	ARG	THR	GLY	LYS

1	MET	HIS	HIS	HIS	HIS	HIS	HIS	LYS	PRO	MET
2	MET	GLU	PRO	LYS	ARG	ILE	ARG	GLU	GLY	TYR
3	LEU	VAL	LYS	LYS	GLY	SER	VAL	PHE	ASN	THR
4	TRP	LYS	PRO	MET	TRP	VAL	VAL	LEU	LEU	GLU
5	ASP	GLY	ILE	GLU	PHE	TYR	LYS	LYS	LYS	SER
6	ASP	ASN	SER	PRO	LYS	GLY	MET	ILE	PRO	LEU
7	LYS	GLY	SER	THR	LEU	THR	SER	PRO	CYS	GLN
8	ASP	PHE	GLY	LYS	ARG	MET	PHE	VAL	PHE	LYS
9	ILE	THR	THR	THR	LYS	GLN	GLN	ASP	HIS	PHE
10	PHE	GLN	ALA	ALA	PHE	LEU	GLU	GLU	ARG	ASP
11	ALA	TRP	VAL	ARG	ASP	ILE	ASN	LYS	ALA	ILE
12	LYS	CYS	ILE	GLU	GLY	GLY	GLN	LYS	PHE	ALA
13	ARG	LYS	SER	THR	ARG	ARG	SER	ILE	ARG	LEU
14	PRO	GLU	THR	ILE	ASP	LEU	GLY	ALA	LEU	TYR
15	LEU	SER	MET	LYS	ASP	THR	GLU	LYS	GLY	ILE
16	LYS	GLU	LEU	ASN	LEU	GLU	LYS	ASP	LYS	LYS
17	ILE	PHE	ASN	HIS	CYS	PHE	THR	GLY	ASN	CYS
18	VAL	ILE	ASP	TRP	LEU	VAL	SER	ASN	GLN	SER
19	VAL	ARG	ASN	ARG	GLN	GLU	GLY	LEU	MET	ILE
20	ALA	SER	SER	LEU	LEU	ASN	GLU	GLY	TYR	LEU
21	GLN	PRO	ALA	GLY	ASP	MET	SER	LYS	SER	ALA
22	VAL	ASP	GLY	THR	ALA	GLU	ASN	PRO	PHE	LEU
23	ASP	ASN	PRO	ASP	ALA	PHE	TYR	TYR	PHE	PRO
24	ASP	SER	GLY	PHE	PHE	CYS	GLU	GLU	ASN	SER
25	SER	ASP	ASP	ASP	VAL	ILE	LEU	LYS	GLU	GLU
26	PHE	ARG	GLY	VAL	ILE	ILE	LYS	GLN	GLY	CYS
27	LEU	LEU	LYS	GLN	GLY	HIS	ARG	ARG	LYS	ASN
28	TRP	LYS	VAL	ARG	LYS	PHE	ILE	LEU	ARG	GLU
29	ASP	PRO	ALA	TYR	LEU	HIS	TYR	TYR	ASP	PRO
30	ALA	GLY	ALA	GLU	ASP	PRO	LEU	GLY	ALA	ILE
31	HIS	LEU	ARG	GLY	CYS	VAL	VAL	THR	SER	VAL
32	GLU	SER	ASN	SER	ASN	GLY	ARG	LYS	SER	GLU
33	GLU	GLU	ASN	LEU	PHE	GLU	ILE	ILE	THR	ALA
34	ASP	GLU	VAL	HIS	TYR	PHE	LEU	GLN	ALA	ALA
35	THR	PRO	LYS	GLU	ARG	THR	GLU	TRP	ILE	LYS
36	ALA	ILE	GLN	MET	ALA	SER	ARG	THR	GLY	LYS