BMRB Entry 5490

Title:
Sequence-specific 1H, 13C, 15N Resonance Assigments of the Major Cherry Allergen Pru av 1 Mutant E45W
Deposition date:
2002-08-05
Original release date:
2003-10-06
Authors:
Neudecker, Philipp; Lehmann, Katrin; Nerkamp, Joerg; Schweimer, Kristian; Sticht, Heirich; Boehm, Markus; Scheurer, Stephan; Vieths, Stefan; Roesch, Paul
Citation:

Citation: Neudecker, Philipp; Lehmann, Katrin; Nerkamp, Joerg; Haase, Tanja; Wangorsch, Andrea; Foetisch, Kay; Hoffmann, Silke; Roesch, Paul; Vieths, Stefan; Scheurer, Stephan. "Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure"  Biochem. J. 376, 97-107 (2003).
PubMed: 12943529

Assembly members:

Assembly members:
Pru av 1, polymer, 159 residues, 17586 Da.

Natural source:

Natural source:   Common Name: sweet cherry   Taxonomy ID: 42229   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Prunus avium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
1H chemical shifts1017
13C chemical shifts558
15N chemical shifts156
coupling constants96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pru av 1 E45W1

Entities:

Entity 1, Pru av 1 E45W 159 residues - 17586 Da.

1   GLYVALPHETHRTYRGLUSERGLUPHETHR
2   SERGLUILEPROPROPROARGLEUPHELYS
3   ALAPHEVALLEUASPALAASPASNLEUVAL
4   PROLYSILEALAPROGLNALAILELYSHIS
5   SERGLUILELEUTRPGLYASPGLYGLYPRO
6   GLYTHRILELYSLYSILETHRPHEGLYGLU
7   GLYSERGLNTYRGLYTYRVALLYSHISLYS
8   ILEASPSERILEASPLYSGLUASNTYRSER
9   TYRSERTYRTHRLEUILEGLUGLYASPALA
10   LEUGLYASPTHRLEUGLULYSILESERTYR
11   GLUTHRLYSLEUVALALASERPROSERGLY
12   GLYSERILEILELYSSERTHRSERHISTYR
13   HISTHRLYSGLYASNVALGLUILELYSGLU
14   GLUHISVALLYSALAGLYLYSGLULYSALA
15   SERASNLEUPHELYSLEUILEGLUTHRTYR
16   LEULYSGLYHISPROASPALATYRASN

Samples:

sample_1: Pru av 1, [U-99% 15N], 0.7 mM

cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablecond_1
1H,15N HSQCsample_1not availablecond_1
HNHAsample_1not availablecond_1
3D 1H,15N-TOCSY-HSQCsample_1not availablecond_1
3D 1H,15N-NOESY-HSQCsample_1not availablecond_1
3D 1H,15N/1H,15N-HMQC-NOESY-HSQCsample_1not availablecond_1

Software:

NDEE - spectral analysis

NMRView v5.0.4 - spectral analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 4671
PDB
EMBL CAM35495
GB AAC02632 ABB78006 ABW99628 ACE80940 EMJ24835
REF XP_007223636
SP O24248
AlphaFold O24248

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks