BMRB Entry 4671

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4671

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Title:
Sequence-specific 1H, 13C and 15N Resonance Assignments of the Major Cherry Allergen Pru a 1
Deposition date:
2000-03-16
Original release date:
2003-03-26
Authors:
Neudecker, Philipp; Nerkamp, Joerg; Schweimer, Kristian; Sticht, Heinrich; Boehm, Markus; Scheurer, Stephan; Vieths, Stefan; Roesch, Paul
Citation:

Citation: Neudecker, Philipp; Schweimer, Kristian; Nerkamp, Joerg; Boehm, Markus; Scheurer, Stephan; Vieths, Stefan; Sticht, Heinrich; Roesch, Paul. "Sequence-specific 1H, 13C and 15N Resonance Assignments of the Major Cherry Allergen Pru a 1"  J. Biomol. NMR 18, 71-72 (2000).

Assembly members:

Assembly members:
Pru a 1, polymer, 159 residues, 17529 Da.

Natural source:

Natural source:   Common Name: sweet cherry   Taxonomy ID: 42229   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Prunus avium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pru a 1: GVFTYESEFTSEIPPPRLFK AFVLDADNLVPKIAPQAIKH SEILEGDGGPGTIKKITFGE GSQYGYVKHKIDSIDKENYS YSYTLIEGDALGDTLEKISY ETKLVASPSGGSIIKSTSHY HTKGNVEIKEEHVKAGKEKA SNLFKLIETYLKGHPDAYN

Data sets:
Data typeCount
1H chemical shifts1015
13C chemical shifts581
15N chemical shifts155
coupling constants97
heteronuclear NOE values133

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pru a 11

Entities:

Entity 1, Pru a 1 159 residues - 17529 Da.

1   GLYVALPHETHRTYRGLUSERGLUPHETHR
2   SERGLUILEPROPROPROARGLEUPHELYS
3   ALAPHEVALLEUASPALAASPASNLEUVAL
4   PROLYSILEALAPROGLNALAILELYSHIS
5   SERGLUILELEUGLUGLYASPGLYGLYPRO
6   GLYTHRILELYSLYSILETHRPHEGLYGLU
7   GLYSERGLNTYRGLYTYRVALLYSHISLYS
8   ILEASPSERILEASPLYSGLUASNTYRSER
9   TYRSERTYRTHRLEUILEGLUGLYASPALA
10   LEUGLYASPTHRLEUGLULYSILESERTYR
11   GLUTHRLYSLEUVALALASERPROSERGLY
12   GLYSERILEILELYSSERTHRSERHISTYR
13   HISTHRLYSGLYASNVALGLUILELYSGLU
14   GLUHISVALLYSALAGLYLYSGLULYSALA
15   SERASNLEUPHELYSLEUILEGLUTHRTYR
16   LEULYSGLYHISPROASPALATYRASN

Samples:

sample_1: Pru a 1, [U-90% 15N], 1.2 mM

sample_2: Pru a 1, [U-95% 13C; U-90% 15N], 0.8 mM

cond_1: pH: 7.0; temperature: 298 K; pressure: 1 atm

cond_2: pH: 7.0; temperature: 308 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
1H,15N HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D 1H,15N-TOCSY-HSQCnot availablenot availablenot available
3D 1H,15N-NOESY-HSQCnot availablenot availablenot available
3D 1H,15N/1H,15N-HMQC-NOESY-HSQCnot availablenot availablenot available
1H,13C CTHSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
H(C)CH-COSYnot availablenot availablenot available
(H)CCH-COSYnot availablenot availablenot available
HC(C)H-TOCSYnot availablenot availablenot available
3D 1H,13C-NOESY-HSQCnot availablenot availablenot available
3D 1H,13C/1H,15N-HMQC-NOESY-HSQCnot availablenot availablenot available

Software:

NDEE - spectral analysis

NMRView v4.1.0 - spectral analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAM35495
GB AAC02632 ABB78006 ABW99628 ABW99631 ABW99632
REF XP_007223636 XP_008223266
SP O24248
AlphaFold O24248

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks