BMRB Entry 5457

Title:
Complete 1H Chemical Shift Assignment of a 24-residue Sin Interacting Domain of hMad1
Deposition date:
2002-07-08
Original release date:
2003-06-02
Authors:
van Ingen, Hugo; Tessari, Marco; Vuister, Geerten
Citation:

Citation: van Ingen, Hugo; Tessari, Marco; Vuister, Geerten. "A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment"  J. Biomol. NMR 24, 155-160 (2002).

Assembly members:

Assembly members:
Mad1, polymer, 24 residues, 2973 Da.
mSin3B, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAH2 domain of mSin3B2
2SID domain of Mad11

Entities:

Entity 2, PAH2 domain of mSin3B 105 residues - Formula weight is not available

1   GLUSERASPSERVALGLUPHEASNASNALA
2   ILESERTYRVALASNLYSILELYSTHRARG
3   PHELEUASPHISPROGLUILETYRARGSER
4   PHELEUGLUILELEUHISTHRTYRGLNLYS
5   GLUGLNLEUHISTHRLYSGLYARGPROPHE
6   ARGGLYMETSERGLUGLUGLUVALPHETHR
7   GLUVALALAASNLEUPHEARGGLYGLNGLU
8   ASPLEULEUSERGLUPHEGLYGLNPHELEU
9   PROGLUALALYSARGSERLEUPHETHRGLY
10   ASNGLYSERALAGLUMETASNSERGLYGLN
11   LYSASNGLUGLULYS

Entity 1, SID domain of Mad1 24 residues - 2973 Da.

1   VALARGMETASNILEGLNMETLEULEUGLU
2   ALAALAASPTYRLEUGLUARGARGGLUARG
3   GLUALAGLUHIS

Samples:

sample_1: Mad1, [U-15N; U-13C], 1.3 mM; mSin3B 1.3 mM; potassium phosphate 50 mM

Ex-cond_1: pH: 6.3; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
The use of a 3D TOCSY-TOCSY was required to solve high degree of overlap insample_1not availableEx-cond_1
the 2D TOCSY spectrum. The overlap is caused by the highly redundant aminosample_1not availableEx-cond_1
acid composition of the C-terminal part of the SIDsample_1not availableEx-cond_1

Software:

NMRPipe -

Xeasy -

NMR spectrometers:

  • Varian UnityInova 600 MHz
  • Varian UnityPlus 800 MHz

Related Database Links:

BMRB 4874 6899 6899 5808 4874 4841
PDB
GB AAH51536 EDL10815 EDL90863 EDL90864
REF XP_006751561
GenBank EDL90864 EDL90863 EDL10815 AAH51536