BMRB Entry 4874

Title:
Sequence-specific assignment of the PAH2 domain of Sin3B free and complexed to Mad1
Deposition date:
2000-10-23
Original release date:
2001-04-26
Authors:
Spronk, Christian; Jansen, Jacobus; Tessari, Marco; Kaan, Anita; Aelen, Jan; Vermeulen, Michiel; Stunnenberg, Hendrik; Vuister, Geerten
Citation:

Citation: Spronk, Christian; Jansen, Jacobus; Tessari, Marco; Kaan, Anita; Aelen, Jan; Lasonder, Edwin; Stunnenberg, Hendrik; Vuister, Geerten. "Letter to the editor: Sequence-specific assignment of the PAH2 domain of Sin3B free and bound to Mad1"  J. Biomol. NMR 19, 377-378 (2001).

Assembly members:

Assembly members:
mSin3B-PAH2 domain, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX2T

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts296
1H chemical shifts102
15N chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAH21

Entities:

Entity 1, PAH2 105 residues - Formula weight is not available

1   GLUSERASPSERVALGLUPHEASNASNALA
2   ILESERTYRVALASNLYSILELYSTHRARG
3   PHELEUASPHISPROGLUILETYRARGSER
4   PHELEUGLUILELEUHISTHRTYRGLNLYS
5   GLUGLNLEUHISTHRLYSGLYARGPROPHE
6   ARGGLYMETSERGLUGLUGLUVALPHETHR
7   GLUVALALAASNLEUPHEARGGLYGLNGLU
8   ASPLEULEUSERGLUPHEGLYGLNPHELEU
9   PROGLUALALYSARGSERLEUPHETHRGLY
10   ASNGLYSERALAGLUMETASNSERGLYGLN
11   LYSASNGLUGLULYS

Samples:

Typical_sample: mSin3B-PAH2 domain, [U-100% 13C; U-100% 15N], 1.0 mM; Potassium Phosphate 50 mM; Potassium Chloride 100 mM

sample_conditions: pH: 6.5 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCATypical_samplenot availablesample_conditions
HNCOTypical_samplenot availablesample_conditions
CBCA(CO)NNHTypical_samplenot availablesample_conditions
HNCACBTypical_samplenot availablesample_conditions

Software:

NMRPipe v1.7 - Data processing

XEASY v1.3.9 - Spectral analysis

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 5457 6899
PDB
GB AAH51536 EDL10815 EDL90863 EDL90864
REF XP_006751561

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks