Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53581

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ERdj4 JD and TD amide backbone assignments

Deposition date:

2026-02-18

Original release date:

2026-09-04

Authors:

Leder, Anna; Mas, Guillaume; Hiller, Sebastian

Citation:

Citation: Leder, Anna; Mas, Guillaume; Hiller, Sebastian. "Deactivation mechanism of the ER stress sensor IRE1a " .

Assembly members:

Assembly members:
entity_1, polymer, 127 residues, Formula weight is not available
entity_2, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SYYDILQVPKSASERQIKKA FHKLAMEYHPDKNKSPDAEA KFREIAEAYETLSDANRRKE YDTLGHSAFTSGKGQRGSGS SFEQSFNFNFDDLFKDFGFF GQNQNTGSKKRFENHFQTRQ DGGSSRQ
entity_2: KHCRTVTQRRGNMVTTYTDC SG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ERdj4 JD	1
2	ERdj4 TD	2

Entities:

Entity 1, ERdj4 JD 127 residues - Formula weight is not available

1

SER

TYR

ASP

ILE

LEU

GLN

VAL

PRO

LYS

2

SER

ALA

SER

GLU

ARG

GLN

ILE

LYS

ALA

3

PHE

HIS

LYS

LEU

ALA

MET

GLU

TYR

HIS

PRO

4

ASP

LYS

ASN

LYS

SER

PRO

ASP

ALA

GLU

ALA

5

LYS

PHE

ARG

GLU

ILE

ALA

GLU

ALA

TYR

GLU

6

THR

LEU

SER

ASP

ALA

ASN

ARG

LYS

GLU

7

TYR

ASP

THR

LEU

GLY

HIS

SER

ALA

PHE

THR

8

SER

GLY

LYS

GLY

GLN

ARG

GLY

SER

GLY

SER

9

SER

PHE

GLU

GLN

SER

PHE

ASN

PHE

ASN

PHE

10

ASP

LEU

PHE

LYS

ASP

PHE

GLY

PHE

11

GLY

GLN

ASN

GLN

ASN

THR

GLY

SER

LYS

12

ARG

PHE

GLU

ASN

HIS

PHE

GLN

THR

ARG

GLN

13

ASP

GLY

SER

ARG

GLN

Entity 2, ERdj4 TD 22 residues - Formula weight is not available

1

LYS

HIS

CYS

ARG

THR

VAL

THR

GLN

ARG

2

GLY

ASN

MET

VAL

THR

TYR

THR

ASP

CYS

3

SER

GLY

Samples:

sample_1: ERdj4 JD, [U-98% 15N], 500 uM; D2O 10%; DSS 0.5 mM; MES 25 mM; KCl 150 mM; MgCl2 10 mM

sample_2: ERdj4 TD, [U-98% 15N], 200 uM; D2O 10%; DSS 0.5 mM; MES 25 mM; KCl 150 mM; MgCl2 10 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

CcpNMR v2.5.2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53581