data_53581 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 53581 _Entry.Title ; ERdj4 JD and TD amide backbone assignments ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2026-02-18 _Entry.Accession_date 2026-02-18 _Entry.Last_release_date 2026-02-18 _Entry.Original_release_date 2026-02-18 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Anna Leder . . . . 53581 2 Guillaume Mas . . . . 53581 3 Sebastian Hiller . . . . 53581 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 2 53581 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 93 53581 '1H chemical shifts' 93 53581 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2026-09-04 . original BMRB . 53581 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 53581 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Deactivation mechanism of the ER stress sensor IRE1a ; _Citation.Status 'in preparation' _Citation.Type journal _Citation.Journal_abbrev 'Not known' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Anna Leder . . . . 53581 1 2 Guillaume Mas . . . . 53581 1 3 Sebastian Hiller . . . . 53581 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 53581 _Assembly.ID 1 _Assembly.Name ERdj4 _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'ERdj4 JD' 1 $entity_1 . . yes native no no . . . 53581 1 2 'ERdj4 TD' 2 $entity_2 . . no native no no . . . 53581 1 stop_ loop_ _Bond.ID _Bond.Type _Bond.Value_order _Bond.Assembly_atom_ID_1 _Bond.Entity_assembly_ID_1 _Bond.Entity_assembly_name_1 _Bond.Entity_ID_1 _Bond.Comp_ID_1 _Bond.Comp_index_ID_1 _Bond.Seq_ID_1 _Bond.Atom_ID_1 _Bond.Assembly_atom_ID_2 _Bond.Entity_assembly_ID_2 _Bond.Entity_assembly_name_2 _Bond.Entity_ID_2 _Bond.Comp_ID_2 _Bond.Comp_index_ID_2 _Bond.Seq_ID_2 _Bond.Atom_ID_2 _Bond.Auth_entity_assembly_ID_1 _Bond.Auth_entity_assembly_name_1 _Bond.Auth_asym_ID_1 _Bond.Auth_seq_ID_1 _Bond.Auth_comp_ID_1 _Bond.Auth_atom_ID_1 _Bond.Auth_entity_assembly_ID_2 _Bond.Auth_entity_assembly_name_2 _Bond.Auth_asym_ID_2 _Bond.Auth_seq_ID_2 _Bond.Auth_comp_ID_2 _Bond.Auth_atom_ID_2 _Bond.Entry_ID _Bond.Assembly_ID 1 disulfide single . 2 . 2 CYS 3 3 SG . 2 . 2 CYS 20 20 SG . . . . . . . . . . . . 53581 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 53581 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; SYYDILQVPKSASERQIKKA FHKLAMEYHPDKNKSPDAEA KFREIAEAYETLSDANRRKE YDTLGHSAFTSGKGQRGSGS SFEQSFNFNFDDLFKDFGFF GQNQNTGSKKRFENHFQTRQ DGGSSRQ ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 127 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 26 SER . 53581 1 2 27 TYR . 53581 1 3 28 TYR . 53581 1 4 29 ASP . 53581 1 5 30 ILE . 53581 1 6 31 LEU . 53581 1 7 32 GLN . 53581 1 8 33 VAL . 53581 1 9 34 PRO . 53581 1 10 35 LYS . 53581 1 11 36 SER . 53581 1 12 37 ALA . 53581 1 13 38 SER . 53581 1 14 39 GLU . 53581 1 15 40 ARG . 53581 1 16 41 GLN . 53581 1 17 42 ILE . 53581 1 18 43 LYS . 53581 1 19 44 LYS . 53581 1 20 45 ALA . 53581 1 21 46 PHE . 53581 1 22 47 HIS . 53581 1 23 48 LYS . 53581 1 24 49 LEU . 53581 1 25 50 ALA . 53581 1 26 51 MET . 53581 1 27 52 GLU . 53581 1 28 53 TYR . 53581 1 29 54 HIS . 53581 1 30 55 PRO . 53581 1 31 56 ASP . 53581 1 32 57 LYS . 53581 1 33 58 ASN . 53581 1 34 59 LYS . 53581 1 35 60 SER . 53581 1 36 61 PRO . 53581 1 37 62 ASP . 53581 1 38 63 ALA . 53581 1 39 64 GLU . 53581 1 40 65 ALA . 53581 1 41 66 LYS . 53581 1 42 67 PHE . 53581 1 43 68 ARG . 53581 1 44 69 GLU . 53581 1 45 70 ILE . 53581 1 46 71 ALA . 53581 1 47 72 GLU . 53581 1 48 73 ALA . 53581 1 49 74 TYR . 53581 1 50 75 GLU . 53581 1 51 76 THR . 53581 1 52 77 LEU . 53581 1 53 78 SER . 53581 1 54 79 ASP . 53581 1 55 80 ALA . 53581 1 56 81 ASN . 53581 1 57 82 ARG . 53581 1 58 83 ARG . 53581 1 59 84 LYS . 53581 1 60 85 GLU . 53581 1 61 86 TYR . 53581 1 62 87 ASP . 53581 1 63 88 THR . 53581 1 64 89 LEU . 53581 1 65 90 GLY . 53581 1 66 91 HIS . 53581 1 67 92 SER . 53581 1 68 93 ALA . 53581 1 69 94 PHE . 53581 1 70 95 THR . 53581 1 71 96 SER . 53581 1 72 97 GLY . 53581 1 73 98 LYS . 53581 1 74 99 GLY . 53581 1 75 100 GLN . 53581 1 76 101 ARG . 53581 1 77 102 GLY . 53581 1 78 103 SER . 53581 1 79 104 GLY . 53581 1 80 105 SER . 53581 1 81 106 SER . 53581 1 82 107 PHE . 53581 1 83 108 GLU . 53581 1 84 109 GLN . 53581 1 85 110 SER . 53581 1 86 111 PHE . 53581 1 87 112 ASN . 53581 1 88 113 PHE . 53581 1 89 114 ASN . 53581 1 90 115 PHE . 53581 1 91 116 ASP . 53581 1 92 117 ASP . 53581 1 93 118 LEU . 53581 1 94 119 PHE . 53581 1 95 120 LYS . 53581 1 96 121 ASP . 53581 1 97 122 PHE . 53581 1 98 123 GLY . 53581 1 99 124 PHE . 53581 1 100 125 PHE . 53581 1 101 126 GLY . 53581 1 102 127 GLN . 53581 1 103 128 ASN . 53581 1 104 129 GLN . 53581 1 105 130 ASN . 53581 1 106 131 THR . 53581 1 107 132 GLY . 53581 1 108 133 SER . 53581 1 109 134 LYS . 53581 1 110 135 LYS . 53581 1 111 136 ARG . 53581 1 112 137 PHE . 53581 1 113 138 GLU . 53581 1 114 139 ASN . 53581 1 115 140 HIS . 53581 1 116 141 PHE . 53581 1 117 142 GLN . 53581 1 118 143 THR . 53581 1 119 144 ARG . 53581 1 120 145 GLN . 53581 1 121 146 ASP . 53581 1 122 147 GLY . 53581 1 123 148 GLY . 53581 1 124 149 SER . 53581 1 125 150 SER . 53581 1 126 151 ARG . 53581 1 127 152 GLN . 53581 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . SER 1 1 53581 1 . TYR 2 2 53581 1 . TYR 3 3 53581 1 . ASP 4 4 53581 1 . ILE 5 5 53581 1 . LEU 6 6 53581 1 . GLN 7 7 53581 1 . VAL 8 8 53581 1 . PRO 9 9 53581 1 . LYS 10 10 53581 1 . SER 11 11 53581 1 . ALA 12 12 53581 1 . SER 13 13 53581 1 . GLU 14 14 53581 1 . ARG 15 15 53581 1 . GLN 16 16 53581 1 . ILE 17 17 53581 1 . LYS 18 18 53581 1 . LYS 19 19 53581 1 . ALA 20 20 53581 1 . PHE 21 21 53581 1 . HIS 22 22 53581 1 . LYS 23 23 53581 1 . LEU 24 24 53581 1 . ALA 25 25 53581 1 . MET 26 26 53581 1 . GLU 27 27 53581 1 . TYR 28 28 53581 1 . HIS 29 29 53581 1 . PRO 30 30 53581 1 . ASP 31 31 53581 1 . LYS 32 32 53581 1 . ASN 33 33 53581 1 . LYS 34 34 53581 1 . SER 35 35 53581 1 . PRO 36 36 53581 1 . ASP 37 37 53581 1 . ALA 38 38 53581 1 . GLU 39 39 53581 1 . ALA 40 40 53581 1 . LYS 41 41 53581 1 . PHE 42 42 53581 1 . ARG 43 43 53581 1 . GLU 44 44 53581 1 . ILE 45 45 53581 1 . ALA 46 46 53581 1 . GLU 47 47 53581 1 . ALA 48 48 53581 1 . TYR 49 49 53581 1 . GLU 50 50 53581 1 . THR 51 51 53581 1 . LEU 52 52 53581 1 . SER 53 53 53581 1 . ASP 54 54 53581 1 . ALA 55 55 53581 1 . ASN 56 56 53581 1 . ARG 57 57 53581 1 . ARG 58 58 53581 1 . LYS 59 59 53581 1 . GLU 60 60 53581 1 . TYR 61 61 53581 1 . ASP 62 62 53581 1 . THR 63 63 53581 1 . LEU 64 64 53581 1 . GLY 65 65 53581 1 . HIS 66 66 53581 1 . SER 67 67 53581 1 . ALA 68 68 53581 1 . PHE 69 69 53581 1 . THR 70 70 53581 1 . SER 71 71 53581 1 . GLY 72 72 53581 1 . LYS 73 73 53581 1 . GLY 74 74 53581 1 . GLN 75 75 53581 1 . ARG 76 76 53581 1 . GLY 77 77 53581 1 . SER 78 78 53581 1 . GLY 79 79 53581 1 . SER 80 80 53581 1 . SER 81 81 53581 1 . PHE 82 82 53581 1 . GLU 83 83 53581 1 . GLN 84 84 53581 1 . SER 85 85 53581 1 . PHE 86 86 53581 1 . ASN 87 87 53581 1 . PHE 88 88 53581 1 . ASN 89 89 53581 1 . PHE 90 90 53581 1 . ASP 91 91 53581 1 . ASP 92 92 53581 1 . LEU 93 93 53581 1 . PHE 94 94 53581 1 . LYS 95 95 53581 1 . ASP 96 96 53581 1 . PHE 97 97 53581 1 . GLY 98 98 53581 1 . PHE 99 99 53581 1 . PHE 100 100 53581 1 . GLY 101 101 53581 1 . GLN 102 102 53581 1 . ASN 103 103 53581 1 . GLN 104 104 53581 1 . ASN 105 105 53581 1 . THR 106 106 53581 1 . GLY 107 107 53581 1 . SER 108 108 53581 1 . LYS 109 109 53581 1 . LYS 110 110 53581 1 . ARG 111 111 53581 1 . PHE 112 112 53581 1 . GLU 113 113 53581 1 . ASN 114 114 53581 1 . HIS 115 115 53581 1 . PHE 116 116 53581 1 . GLN 117 117 53581 1 . THR 118 118 53581 1 . ARG 119 119 53581 1 . GLN 120 120 53581 1 . ASP 121 121 53581 1 . GLY 122 122 53581 1 . GLY 123 123 53581 1 . SER 124 124 53581 1 . SER 125 125 53581 1 . ARG 126 126 53581 1 . GLN 127 127 53581 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 53581 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; KHCRTVTQRRGNMVTTYTDC SG ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 22 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all disulfide bound' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 201 LYS . 53581 2 2 202 HIS . 53581 2 3 203 CYS . 53581 2 4 204 ARG . 53581 2 5 205 THR . 53581 2 6 206 VAL . 53581 2 7 207 THR . 53581 2 8 208 GLN . 53581 2 9 209 ARG . 53581 2 10 210 ARG . 53581 2 11 211 GLY . 53581 2 12 212 ASN . 53581 2 13 213 MET . 53581 2 14 214 VAL . 53581 2 15 215 THR . 53581 2 16 216 THR . 53581 2 17 217 TYR . 53581 2 18 218 THR . 53581 2 19 219 ASP . 53581 2 20 220 CYS . 53581 2 21 221 SER . 53581 2 22 222 GLY . 53581 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . LYS 1 1 53581 2 . HIS 2 2 53581 2 . CYS 3 3 53581 2 . ARG 4 4 53581 2 . THR 5 5 53581 2 . VAL 6 6 53581 2 . THR 7 7 53581 2 . GLN 8 8 53581 2 . ARG 9 9 53581 2 . ARG 10 10 53581 2 . GLY 11 11 53581 2 . ASN 12 12 53581 2 . MET 13 13 53581 2 . VAL 14 14 53581 2 . THR 15 15 53581 2 . THR 16 16 53581 2 . TYR 17 17 53581 2 . THR 18 18 53581 2 . ASP 19 19 53581 2 . CYS 20 20 53581 2 . SER 21 21 53581 2 . GLY 22 22 53581 2 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 53581 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 53581 1 2 2 $entity_2 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 53581 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 53581 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET-28A . . . 53581 1 2 2 $entity_2 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET-28A . . . 53581 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 53581 _Sample.ID 1 _Sample.Name 'ERdj4 JD' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'ERdj4 JD' '[U-98% 15N]' . . 1 $entity_1 . . 500 . . uM . . . . 53581 1 2 D2O 'natural abundance' . . . . . . 10 . . % . . . . 53581 1 3 DSS 'natural abundance' . . . . . . 0.5 . . mM . . . . 53581 1 4 MES 'natural abundance' . . . . . . 25 . . mM . . . . 53581 1 5 KCl 'natural abundance' . . . . . . 150 . . mM . . . . 53581 1 6 MgCl2 'natural abundance' . . . . . . 10 . . mM . . . . 53581 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 53581 _Sample.ID 2 _Sample.Name 'ERdj4 TD' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'ERdj4 TD' '[U-98% 15N]' . . 2 $entity_2 . . 200 . . uM . . . . 53581 2 2 D2O 'natural abundance' . . . . . . 10 . . % . . . . 53581 2 3 DSS 'natural abundance' . . . . . . 0.5 . . mM . . . . 53581 2 4 MES 'natural abundance' . . . . . . 25 . . mM . . . . 53581 2 5 KCl 'natural abundance' . . . . . . 150 . . mM . . . . 53581 2 6 MgCl2 'natural abundance' . . . . . . 10 . . mM . . . . 53581 2 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 53581 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Amide backbone assignment ERdj4' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 150 . mM 53581 1 pH 6.5 . pH 53581 1 pressure 1 . atm 53581 1 temperature 298 . K 53581 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 53581 _Software.ID 1 _Software.Type . _Software.Name CcpNMR _Software.Version 2.5.2 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 53581 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 53581 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '700 MHz Bruker' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 53581 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53581 1 2 '2D 1H-15N HSQC' no no no . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53581 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 53581 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'ERdj4 JD amide backbone assignment' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0 internal direct 1 . . . . . 53581 1 N 15 DSS 'methyl protons' . . . . ppm 0 internal indirect . . . . . . 53581 1 stop_ save_ save_chem_shift_reference_2 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_2 _Chem_shift_reference.Entry_ID 53581 _Chem_shift_reference.ID 2 _Chem_shift_reference.Name 'ERdj4 TD amide backbone assignment' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0 internal direct 1 . . . . . 53581 2 N 15 DSS 'methyl protons' . . . . ppm 0 internal indirect . . . . . . 53581 2 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 53581 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'ERdj4 JD amide backbone assignment' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 53581 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 53581 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 SER H H 1 8.581331965 . . 1 . . . . . 26 SER H . 53581 1 2 . 1 . 1 1 1 SER N N 15 117.1950012 . . 1 . . . . . 26 SER N . 53581 1 3 . 1 . 1 2 2 TYR H H 1 9.004464927 . . 1 . . . . . 27 TYR H . 53581 1 4 . 1 . 1 2 2 TYR N N 15 122.712882 . . 1 . . . . . 27 TYR N . 53581 1 5 . 1 . 1 3 3 TYR H H 1 7.152550169 . . 1 . . . . . 28 TYR H . 53581 1 6 . 1 . 1 3 3 TYR N N 15 116.3868674 . . 1 . . . . . 28 TYR N . 53581 1 7 . 1 . 1 4 4 ASP H H 1 7.264793501 . . 1 . . . . . 29 ASP H . 53581 1 8 . 1 . 1 4 4 ASP N N 15 120.2749966 . . 1 . . . . . 29 ASP N . 53581 1 9 . 1 . 1 5 5 ILE H H 1 8.033704476 . . 1 . . . . . 30 ILE H . 53581 1 10 . 1 . 1 5 5 ILE N N 15 121.8433091 . . 1 . . . . . 30 ILE N . 53581 1 11 . 1 . 1 6 6 LEU H H 1 6.975464658 . . 1 . . . . . 31 LEU H . 53581 1 12 . 1 . 1 6 6 LEU N N 15 115.6083382 . . 1 . . . . . 31 LEU N . 53581 1 13 . 1 . 1 7 7 GLN H H 1 7.77764 . . 1 . . . . . 32 GLN H . 53581 1 14 . 1 . 1 7 7 GLN N N 15 109.2694576 . . 1 . . . . . 32 GLN N . 53581 1 15 . 1 . 1 8 8 VAL H H 1 7.856362446 . . 1 . . . . . 33 VAL H . 53581 1 16 . 1 . 1 8 8 VAL N N 15 111.6961259 . . 1 . . . . . 33 VAL N . 53581 1 17 . 1 . 1 10 10 LYS H H 1 8.043769629 . . 1 . . . . . 35 LYS H . 53581 1 18 . 1 . 1 10 10 LYS N N 15 119.7022237 . . 1 . . . . . 35 LYS N . 53581 1 19 . 1 . 1 11 11 SER H H 1 6.990958849 . . 1 . . . . . 36 SER H . 53581 1 20 . 1 . 1 11 11 SER N N 15 108.2894619 . . 1 . . . . . 36 SER N . 53581 1 21 . 1 . 1 12 12 ALA H H 1 7.506210622 . . 1 . . . . . 37 ALA H . 53581 1 22 . 1 . 1 12 12 ALA N N 15 124.568322 . . 1 . . . . . 37 ALA N . 53581 1 23 . 1 . 1 13 13 SER H H 1 8.588900991 . . 1 . . . . . 38 SER H . 53581 1 24 . 1 . 1 13 13 SER N N 15 118.057045 . . 1 . . . . . 38 SER N . 53581 1 25 . 1 . 1 14 14 GLU H H 1 8.992750236 . . 1 . . . . . 39 GLU H . 53581 1 26 . 1 . 1 14 14 GLU N N 15 122.1299939 . . 1 . . . . . 39 GLU N . 53581 1 27 . 1 . 1 15 15 ARG H H 1 8.230857943 . . 1 . . . . . 40 ARG H . 53581 1 28 . 1 . 1 15 15 ARG N N 15 119.3182054 . . 1 . . . . . 40 ARG N . 53581 1 29 . 1 . 1 16 16 GLN H H 1 7.744287514 . . 1 . . . . . 41 GLN H . 53581 1 30 . 1 . 1 16 16 GLN N N 15 120.7167689 . . 1 . . . . . 41 GLN N . 53581 1 31 . 1 . 1 17 17 ILE H H 1 8.107232279 . . 1 . . . . . 42 ILE H . 53581 1 32 . 1 . 1 17 17 ILE N N 15 122.7401561 . . 1 . . . . . 42 ILE N . 53581 1 33 . 1 . 1 18 18 LYS H H 1 8.14031647 . . 1 . . . . . 43 LYS H . 53581 1 34 . 1 . 1 18 18 LYS N N 15 120.3596591 . . 1 . . . . . 43 LYS N . 53581 1 35 . 1 . 1 19 19 LYS H H 1 8.078578072 . . 1 . . . . . 44 LYS H . 53581 1 36 . 1 . 1 19 19 LYS N N 15 119.5121504 . . 1 . . . . . 44 LYS N . 53581 1 37 . 1 . 1 20 20 ALA H H 1 7.893465708 . . 1 . . . . . 45 ALA H . 53581 1 38 . 1 . 1 20 20 ALA N N 15 122.7183755 . . 1 . . . . . 45 ALA N . 53581 1 39 . 1 . 1 21 21 PHE H H 1 8.26 . . 1 . . . . . 46 PHE H . 53581 1 40 . 1 . 1 21 21 PHE N N 15 117.783 . . 1 . . . . . 46 PHE N . 53581 1 41 . 1 . 1 22 22 HIS H H 1 8.179 . . 1 . . . . . 47 HIS H . 53581 1 42 . 1 . 1 22 22 HIS N N 15 117.952 . . 1 . . . . . 47 HIS N . 53581 1 43 . 1 . 1 23 23 LYS H H 1 7.585402486 . . 1 . . . . . 48 LYS H . 53581 1 44 . 1 . 1 23 23 LYS N N 15 118.1905084 . . 1 . . . . . 48 LYS N . 53581 1 45 . 1 . 1 24 24 LEU H H 1 7.783248782 . . 1 . . . . . 49 LEU H . 53581 1 46 . 1 . 1 24 24 LEU N N 15 120.8876723 . . 1 . . . . . 49 LEU N . 53581 1 47 . 1 . 1 25 25 ALA H H 1 8.951627386 . . 1 . . . . . 50 ALA H . 53581 1 48 . 1 . 1 25 25 ALA N N 15 123.6784022 . . 1 . . . . . 50 ALA N . 53581 1 49 . 1 . 1 26 26 MET H H 1 7.175335102 . . 1 . . . . . 51 MET H . 53581 1 50 . 1 . 1 26 26 MET N N 15 114.3598581 . . 1 . . . . . 51 MET N . 53581 1 51 . 1 . 1 27 27 GLU H H 1 7.017079996 . . 1 . . . . . 52 GLU H . 53581 1 52 . 1 . 1 27 27 GLU N N 15 117.8500696 . . 1 . . . . . 52 GLU N . 53581 1 53 . 1 . 1 28 28 TYR H H 1 7.75847803 . . 1 . . . . . 53 TYR H . 53581 1 54 . 1 . 1 28 28 TYR N N 15 114.7978252 . . 1 . . . . . 53 TYR N . 53581 1 55 . 1 . 1 29 29 HIS H H 1 7.513314738 . . 1 . . . . . 54 HIS H . 53581 1 56 . 1 . 1 29 29 HIS N N 15 122.0352906 . . 1 . . . . . 54 HIS N . 53581 1 57 . 1 . 1 32 32 LYS H H 1 7.895827994 . . 1 . . . . . 57 LYS H . 53581 1 58 . 1 . 1 32 32 LYS N N 15 117.7748281 . . 1 . . . . . 57 LYS N . 53581 1 59 . 1 . 1 33 33 ASN H H 1 7.955328846 . . 1 . . . . . 58 ASN H . 53581 1 60 . 1 . 1 33 33 ASN N N 15 119.9236693 . . 1 . . . . . 58 ASN N . 53581 1 61 . 1 . 1 34 34 LYS H H 1 8.400500663 . . 1 . . . . . 59 LYS H . 53581 1 62 . 1 . 1 34 34 LYS N N 15 122.6398259 . . 1 . . . . . 59 LYS N . 53581 1 63 . 1 . 1 35 35 SER H H 1 8.170248338 . . 1 . . . . . 60 SER H . 53581 1 64 . 1 . 1 35 35 SER N N 15 118.7376414 . . 1 . . . . . 60 SER N . 53581 1 65 . 1 . 1 37 37 ASP H H 1 8.055789494 . . 1 . . . . . 62 ASP H . 53581 1 66 . 1 . 1 37 37 ASP N N 15 116.2097109 . . 1 . . . . . 62 ASP N . 53581 1 67 . 1 . 1 38 38 ALA H H 1 7.541548122 . . 1 . . . . . 63 ALA H . 53581 1 68 . 1 . 1 38 38 ALA N N 15 123.4870575 . . 1 . . . . . 63 ALA N . 53581 1 69 . 1 . 1 39 39 GLU H H 1 8.219 . . 1 . . . . . 64 GLU H . 53581 1 70 . 1 . 1 39 39 GLU N N 15 117.953 . . 1 . . . . . 64 GLU N . 53581 1 71 . 1 . 1 40 40 ALA H H 1 7.912006287 . . 1 . . . . . 65 ALA H . 53581 1 72 . 1 . 1 40 40 ALA N N 15 122.3168897 . . 1 . . . . . 65 ALA N . 53581 1 73 . 1 . 1 41 41 LYS H H 1 7.765253015 . . 1 . . . . . 66 LYS H . 53581 1 74 . 1 . 1 41 41 LYS N N 15 119.4627622 . . 1 . . . . . 66 LYS N . 53581 1 75 . 1 . 1 42 42 PHE H H 1 8.771924799 . . 1 . . . . . 67 PHE H . 53581 1 76 . 1 . 1 42 42 PHE N N 15 120.4810223 . . 1 . . . . . 67 PHE N . 53581 1 77 . 1 . 1 43 43 ARG H H 1 8.065182197 . . 1 . . . . . 68 ARG H . 53581 1 78 . 1 . 1 43 43 ARG N N 15 119.0282187 . . 1 . . . . . 68 ARG N . 53581 1 79 . 1 . 1 44 44 GLU H H 1 7.314510211 . . 1 . . . . . 69 GLU H . 53581 1 80 . 1 . 1 44 44 GLU N N 15 119.0916068 . . 1 . . . . . 69 GLU N . 53581 1 81 . 1 . 1 45 45 ILE H H 1 7.415334287 . . 1 . . . . . 70 ILE H . 53581 1 82 . 1 . 1 45 45 ILE N N 15 120.2505725 . . 1 . . . . . 70 ILE N . 53581 1 83 . 1 . 1 46 46 ALA H H 1 8.267620795 . . 1 . . . . . 71 ALA H . 53581 1 84 . 1 . 1 46 46 ALA N N 15 123.5689579 . . 1 . . . . . 71 ALA N . 53581 1 85 . 1 . 1 47 47 GLU H H 1 7.799972364 . . 1 . . . . . 72 GLU H . 53581 1 86 . 1 . 1 47 47 GLU N N 15 119.9910244 . . 1 . . . . . 72 GLU N . 53581 1 87 . 1 . 1 48 48 ALA H H 1 7.394096386 . . 1 . . . . . 73 ALA H . 53581 1 88 . 1 . 1 48 48 ALA N N 15 122.8649928 . . 1 . . . . . 73 ALA N . 53581 1 89 . 1 . 1 49 49 TYR H H 1 8.217 . . 1 . . . . . 74 TYR H . 53581 1 90 . 1 . 1 49 49 TYR N N 15 118.202 . . 1 . . . . . 74 TYR N . 53581 1 91 . 1 . 1 50 50 GLU H H 1 8.310397735 . . 1 . . . . . 75 GLU H . 53581 1 92 . 1 . 1 50 50 GLU N N 15 122.1286712 . . 1 . . . . . 75 GLU N . 53581 1 93 . 1 . 1 51 51 THR H H 1 6.532088465 . . 1 . . . . . 76 THR H . 53581 1 94 . 1 . 1 51 51 THR N N 15 112.9652922 . . 1 . . . . . 76 THR N . 53581 1 95 . 1 . 1 52 52 LEU H H 1 7.421393497 . . 1 . . . . . 77 LEU H . 53581 1 96 . 1 . 1 52 52 LEU N N 15 113.9571886 . . 1 . . . . . 77 LEU N . 53581 1 97 . 1 . 1 53 53 SER H H 1 7.627465906 . . 1 . . . . . 78 SER H . 53581 1 98 . 1 . 1 53 53 SER N N 15 109.541911 . . 1 . . . . . 78 SER N . 53581 1 99 . 1 . 1 54 54 ASP H H 1 6.62358295 . . 1 . . . . . 79 ASP H . 53581 1 100 . 1 . 1 54 54 ASP N N 15 124.4723424 . . 1 . . . . . 79 ASP N . 53581 1 101 . 1 . 1 55 55 ALA H H 1 8.787194043 . . 1 . . . . . 80 ALA H . 53581 1 102 . 1 . 1 55 55 ALA N N 15 128.6736693 . . 1 . . . . . 80 ALA N . 53581 1 103 . 1 . 1 56 56 ASN H H 1 8.028895766 . . 1 . . . . . 81 ASN H . 53581 1 104 . 1 . 1 56 56 ASN N N 15 115.986241 . . 1 . . . . . 81 ASN N . 53581 1 105 . 1 . 1 57 57 ARG H H 1 8.489808862 . . 1 . . . . . 82 ARG H . 53581 1 106 . 1 . 1 57 57 ARG N N 15 124.1388277 . . 1 . . . . . 82 ARG N . 53581 1 107 . 1 . 1 58 58 ARG H H 1 9.194734321 . . 1 . . . . . 83 ARG H . 53581 1 108 . 1 . 1 58 58 ARG N N 15 121.9616019 . . 1 . . . . . 83 ARG N . 53581 1 109 . 1 . 1 59 59 LYS H H 1 7.362801217 . . 1 . . . . . 84 LYS H . 53581 1 110 . 1 . 1 59 59 LYS N N 15 118.2635113 . . 1 . . . . . 84 LYS N . 53581 1 111 . 1 . 1 60 60 GLU H H 1 7.500343977 . . 1 . . . . . 85 GLU H . 53581 1 112 . 1 . 1 60 60 GLU N N 15 122.0685437 . . 1 . . . . . 85 GLU N . 53581 1 113 . 1 . 1 61 61 TYR H H 1 8.534312735 . . 1 . . . . . 86 TYR H . 53581 1 114 . 1 . 1 61 61 TYR N N 15 123.3899921 . . 1 . . . . . 86 TYR N . 53581 1 115 . 1 . 1 62 62 ASP H H 1 8.90340835 . . 1 . . . . . 87 ASP H . 53581 1 116 . 1 . 1 62 62 ASP N N 15 119.779703 . . 1 . . . . . 87 ASP N . 53581 1 117 . 1 . 1 63 63 THR H H 1 7.851596863 . . 1 . . . . . 88 THR H . 53581 1 118 . 1 . 1 63 63 THR N N 15 116.0763231 . . 1 . . . . . 88 THR N . 53581 1 119 . 1 . 1 64 64 LEU H H 1 8.383533355 . . 1 . . . . . 89 LEU H . 53581 1 120 . 1 . 1 64 64 LEU N N 15 121.657178 . . 1 . . . . . 89 LEU N . 53581 1 121 . 1 . 1 65 65 GLY H H 1 7.619044887 . . 1 . . . . . 90 GLY H . 53581 1 122 . 1 . 1 65 65 GLY N N 15 107.8955921 . . 1 . . . . . 90 GLY N . 53581 1 123 . 1 . 1 66 66 HIS H H 1 8.035250533 . . 1 . . . . . 91 HIS H . 53581 1 124 . 1 . 1 66 66 HIS N N 15 118.9152815 . . 1 . . . . . 91 HIS N . 53581 1 125 . 1 . 1 68 68 ALA H H 1 8.592149289 . . 1 . . . . . 93 ALA H . 53581 1 126 . 1 . 1 68 68 ALA N N 15 124.6810192 . . 1 . . . . . 93 ALA N . 53581 1 127 . 1 . 1 69 69 PHE H H 1 8.296762967 . . 1 . . . . . 94 PHE H . 53581 1 128 . 1 . 1 69 69 PHE N N 15 118.798385 . . 1 . . . . . 94 PHE N . 53581 1 129 . 1 . 1 70 70 THR H H 1 7.938241505 . . 1 . . . . . 95 THR H . 53581 1 130 . 1 . 1 70 70 THR N N 15 109.3253004 . . 1 . . . . . 95 THR N . 53581 1 131 . 1 . 1 71 71 SER H H 1 7.659822968 . . 1 . . . . . 96 SER H . 53581 1 132 . 1 . 1 71 71 SER N N 15 117.9148275 . . 1 . . . . . 96 SER N . 53581 1 133 . 1 . 1 72 72 GLY H H 1 7.800552987 . . 1 . . . . . 97 GLY H . 53581 1 134 . 1 . 1 72 72 GLY N N 15 110.292505 . . 1 . . . . . 97 GLY N . 53581 1 135 . 1 . 1 73 73 LYS H H 1 7.670334367 . . 1 . . . . . 98 LYS H . 53581 1 136 . 1 . 1 73 73 LYS N N 15 120.8349958 . . 1 . . . . . 98 LYS N . 53581 1 137 . 1 . 1 74 74 GLY H H 1 8.199300715 . . 1 . . . . . 99 GLY H . 53581 1 138 . 1 . 1 74 74 GLY N N 15 109.6000124 . . 1 . . . . . 99 GLY N . 53581 1 139 . 1 . 1 75 75 GLN H H 1 8.017436334 . . 1 . . . . . 100 GLN H . 53581 1 140 . 1 . 1 75 75 GLN N N 15 120.3786712 . . 1 . . . . . 100 GLN N . 53581 1 141 . 1 . 1 76 76 ARG H H 1 8.203332735 . . 1 . . . . . 101 ARG H . 53581 1 142 . 1 . 1 76 76 ARG N N 15 122.3717279 . . 1 . . . . . 101 ARG N . 53581 1 stop_ save_ save_assigned_chemical_shifts_2 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_2 _Assigned_chem_shift_list.Entry_ID 53581 _Assigned_chem_shift_list.ID 2 _Assigned_chem_shift_list.Name 'ERdj4 TD amide backbone assignment' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 2 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_2 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 2 '2D 1H-15N HSQC' . . . 53581 2 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 53581 2 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 2 . 2 1 1 LYS H H 1 8.22796 . . 1 . . . . . 201 LYS H . 53581 2 2 . 2 . 2 1 1 LYS N N 15 122.8379 . . 1 . . . . . 201 LYS N . 53581 2 3 . 2 . 2 2 2 HIS H H 1 8.42787 . . 1 . . . . . 202 HIS H . 53581 2 4 . 2 . 2 2 2 HIS N N 15 120.36402 . . 1 . . . . . 202 HIS N . 53581 2 5 . 2 . 2 3 3 CYS H H 1 8.28356 . . 1 . . . . . 203 CYS H . 53581 2 6 . 2 . 2 3 3 CYS N N 15 120.48194 . . 1 . . . . . 203 CYS N . 53581 2 7 . 2 . 2 4 4 ARG H H 1 8.58992 . . 1 . . . . . 204 ARG H . 53581 2 8 . 2 . 2 4 4 ARG N N 15 121.64779 . . 1 . . . . . 204 ARG N . 53581 2 9 . 2 . 2 5 5 THR H H 1 8.57601 . . 1 . . . . . 205 THR H . 53581 2 10 . 2 . 2 5 5 THR N N 15 119.04313 . . 1 . . . . . 205 THR N . 53581 2 11 . 2 . 2 6 6 VAL H H 1 8.92383 . . 1 . . . . . 206 VAL H . 53581 2 12 . 2 . 2 6 6 VAL N N 15 128.0467 . . 1 . . . . . 206 VAL N . 53581 2 13 . 2 . 2 7 7 THR H H 1 8.28044 . . 1 . . . . . 207 THR H . 53581 2 14 . 2 . 2 7 7 THR N N 15 119.73011 . . 1 . . . . . 207 THR N . 53581 2 15 . 2 . 2 8 8 GLN H H 1 8.96738 . . 1 . . . . . 208 GLN H . 53581 2 16 . 2 . 2 8 8 GLN N N 15 125.09936 . . 1 . . . . . 208 GLN N . 53581 2 17 . 2 . 2 9 9 ARG H H 1 8.70838 . . 1 . . . . . 209 ARG H . 53581 2 18 . 2 . 2 9 9 ARG N N 15 124.93877 . . 1 . . . . . 209 ARG N . 53581 2 19 . 2 . 2 10 10 ARG H H 1 8.53779 . . 1 . . . . . 210 ARG H . 53581 2 20 . 2 . 2 10 10 ARG N N 15 126.64125 . . 1 . . . . . 210 ARG N . 53581 2 21 . 2 . 2 11 11 GLY H H 1 8.91886 . . 1 . . . . . 211 GLY H . 53581 2 22 . 2 . 2 11 11 GLY N N 15 115.98086 . . 1 . . . . . 211 GLY N . 53581 2 23 . 2 . 2 12 12 ASN H H 1 8.75171 . . 1 . . . . . 212 ASN H . 53581 2 24 . 2 . 2 12 12 ASN N N 15 123.31767 . . 1 . . . . . 212 ASN N . 53581 2 25 . 2 . 2 13 13 MET H H 1 7.97142 . . 1 . . . . . 213 MET H . 53581 2 26 . 2 . 2 13 13 MET N N 15 119.78054 . . 1 . . . . . 213 MET N . 53581 2 27 . 2 . 2 14 14 VAL H H 1 8.48599 . . 1 . . . . . 214 VAL H . 53581 2 28 . 2 . 2 14 14 VAL N N 15 124.65516 . . 1 . . . . . 214 VAL N . 53581 2 29 . 2 . 2 15 15 THR H H 1 8.69255 . . 1 . . . . . 215 THR H . 53581 2 30 . 2 . 2 15 15 THR N N 15 121.45565 . . 1 . . . . . 215 THR N . 53581 2 31 . 2 . 2 16 16 THR H H 1 8.33435 . . 1 . . . . . 216 THR H . 53581 2 32 . 2 . 2 16 16 THR N N 15 117.35793 . . 1 . . . . . 216 THR N . 53581 2 33 . 2 . 2 17 17 TYR H H 1 8.77044 . . 1 . . . . . 217 TYR H . 53581 2 34 . 2 . 2 17 17 TYR N N 15 122.44109 . . 1 . . . . . 217 TYR N . 53581 2 35 . 2 . 2 18 18 THR H H 1 8.50656 . . 1 . . . . . 218 THR H . 53581 2 36 . 2 . 2 18 18 THR N N 15 117.07299 . . 1 . . . . . 218 THR N . 53581 2 37 . 2 . 2 19 19 ASP H H 1 8.9387 . . 1 . . . . . 219 ASP H . 53581 2 38 . 2 . 2 19 19 ASP N N 15 126.70637 . . 1 . . . . . 219 ASP N . 53581 2 39 . 2 . 2 20 20 CYS H H 1 8.94674 . . 1 . . . . . 220 CYS H . 53581 2 40 . 2 . 2 20 20 CYS N N 15 124.57872 . . 1 . . . . . 220 CYS N . 53581 2 41 . 2 . 2 21 21 SER H H 1 8.51751 . . 1 . . . . . 221 SER H . 53581 2 42 . 2 . 2 21 21 SER N N 15 117.9187 . . 1 . . . . . 221 SER N . 53581 2 43 . 2 . 2 22 22 GLY H H 1 8.1361 . . 1 . . . . . 222 GLY H . 53581 2 44 . 2 . 2 22 22 GLY N N 15 116.56515 . . 1 . . . . . 222 GLY N . 53581 2 stop_ save_