BMRB Entry 53516

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53516

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Title:
Partial backbone assignments for human Golph3
Deposition date:
2026-01-30
Original release date:
2026-03-02
Authors:
Abriata, Luciano
Citation:

Citation: Theodoropoulou, Anastasia; Nasrallah, Anita; Abriata, Luciano; Dal Peraro, Matteo; D'Angelo, Giovanni. "Molecular Regulation and Physiological Role of GOLPH3-mediated Golgi retention"  Nat. commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 249 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Golph3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKGDSKETRLTLMEEVLLL GLKDREGYTSFWNDCISSGL RGCMLIELALRGRLQLEACG MRRKSLLTRKVICKSDAPTG DVLLDEALKHVKETQPPETV QNWIELLSGETWNPLKLHYQ LRNVRERLAKNLVEKGVLTT EKQNFGGGDMTTHPLTNNNI KQRLIKKVQEAVLDKWVNDP HRMDRRLLALIYLAHASDVL ENAFAPLLDEQYDLATKRVR QLLDLDPEVECLKANTNEVL WAVVAAFTK

Data sets:
Data typeCount
13C chemical shifts60
15N chemical shifts27
1H chemical shifts27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Golph31

Entities:

Entity 1, Golph3 249 residues - Formula weight is not available

1   GLYSERLYSGLYASPSERLYSGLUTHRARG
2   LEUTHRLEUMETGLUGLUVALLEULEULEU
3   GLYLEULYSASPARGGLUGLYTYRTHRSER
4   PHETRPASNASPCYSILESERSERGLYLEU
5   ARGGLYCYSMETLEUILEGLULEUALALEU
6   ARGGLYARGLEUGLNLEUGLUALACYSGLY
7   METARGARGLYSSERLEULEUTHRARGLYS
8   VALILECYSLYSSERASPALAPROTHRGLY
9   ASPVALLEULEUASPGLUALALEULYSHIS
10   VALLYSGLUTHRGLNPROPROGLUTHRVAL
11   GLNASNTRPILEGLULEULEUSERGLYGLU
12   THRTRPASNPROLEULYSLEUHISTYRGLN
13   LEUARGASNVALARGGLUARGLEUALALYS
14   ASNLEUVALGLULYSGLYVALLEUTHRTHR
15   GLULYSGLNASNPHEGLYGLYGLYASPMET
16   THRTHRHISPROLEUTHRASNASNASNILE
17   LYSGLNARGLEUILELYSLYSVALGLNGLU
18   ALAVALLEUASPLYSTRPVALASNASPPRO
19   HISARGMETASPARGARGLEULEUALALEU
20   ILETYRLEUALAHISALASERASPVALLEU
21   GLUASNALAPHEALAPROLEULEUASPGLU
22   GLNTYRASPLEUALATHRLYSARGVALARG
23   GLNLEULEUASPLEUASPPROGLUVALGLU
24   CYSLEULYSALAASNTHRASNGLUVALLEU
25   TRPALAVALVALALAALAPHETHRLYS

Samples:

sample_1: Golph3, [U-99% 13C; U-99% 15N], 300 uM; H2O 90%; D2O 10%; MES 50 mM; sodium chloride 225 mM

sample_conditions_1: ionic strength: 225 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks