data_53516 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 53516 _Entry.Title ; Partial backbone assignments for human Golph3 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2026-01-30 _Entry.Accession_date 2026-01-30 _Entry.Last_release_date 2026-02-03 _Entry.Original_release_date 2026-02-03 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details 'Partial 1H,15N,13CA and 13CB assignments' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Luciano Abriata . A. . 0000-0003-3087-8677 53516 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 53516 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 60 53516 '15N chemical shifts' 27 53516 '1H chemical shifts' 27 53516 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2026-03-02 . original BMRB . 53516 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 53516 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Molecular Regulation and Physiological Role of GOLPH3-mediated Golgi retention ; _Citation.Status submitted _Citation.Type journal _Citation.Journal_abbrev 'Nat. commun.' _Citation.Journal_name_full 'Nature communications' _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details 'Revised version to be submitted' loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Anastasia Theodoropoulou . . . . 53516 1 2 Anita Nasrallah . . . . 53516 1 3 Luciano Abriata . . . . 53516 1 4 Matteo 'Dal Peraro' . . . . 53516 1 5 Giovanni D'Angelo . . . . 53516 1 stop_ loop_ _Citation_keyword.Keyword _Citation_keyword.Entry_ID _Citation_keyword.Citation_ID 'golph3, golgi phosphoprotein, golgi biochemistry' 53516 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 53516 _Assembly.ID 1 _Assembly.Name Golph3 _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 Golph3 1 $entity_1 . . yes native no no . . . 53516 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 53516 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GSKGDSKETRLTLMEEVLLL GLKDREGYTSFWNDCISSGL RGCMLIELALRGRLQLEACG MRRKSLLTRKVICKSDAPTG DVLLDEALKHVKETQPPETV QNWIELLSGETWNPLKLHYQ LRNVRERLAKNLVEKGVLTT EKQNFGGGDMTTHPLTNNNI KQRLIKKVQEAVLDKWVNDP HRMDRRLLALIYLAHASDVL ENAFAPLLDEQYDLATKRVR QLLDLDPEVECLKANTNEVL WAVVAAFTK ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 249 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 50 GLY . 53516 1 2 51 SER . 53516 1 3 52 LYS . 53516 1 4 53 GLY . 53516 1 5 54 ASP . 53516 1 6 55 SER . 53516 1 7 56 LYS . 53516 1 8 57 GLU . 53516 1 9 58 THR . 53516 1 10 59 ARG . 53516 1 11 60 LEU . 53516 1 12 61 THR . 53516 1 13 62 LEU . 53516 1 14 63 MET . 53516 1 15 64 GLU . 53516 1 16 65 GLU . 53516 1 17 66 VAL . 53516 1 18 67 LEU . 53516 1 19 68 LEU . 53516 1 20 69 LEU . 53516 1 21 70 GLY . 53516 1 22 71 LEU . 53516 1 23 72 LYS . 53516 1 24 73 ASP . 53516 1 25 74 ARG . 53516 1 26 75 GLU . 53516 1 27 76 GLY . 53516 1 28 77 TYR . 53516 1 29 78 THR . 53516 1 30 79 SER . 53516 1 31 80 PHE . 53516 1 32 81 TRP . 53516 1 33 82 ASN . 53516 1 34 83 ASP . 53516 1 35 84 CYS . 53516 1 36 85 ILE . 53516 1 37 86 SER . 53516 1 38 87 SER . 53516 1 39 88 GLY . 53516 1 40 89 LEU . 53516 1 41 90 ARG . 53516 1 42 91 GLY . 53516 1 43 92 CYS . 53516 1 44 93 MET . 53516 1 45 94 LEU . 53516 1 46 95 ILE . 53516 1 47 96 GLU . 53516 1 48 97 LEU . 53516 1 49 98 ALA . 53516 1 50 99 LEU . 53516 1 51 100 ARG . 53516 1 52 101 GLY . 53516 1 53 102 ARG . 53516 1 54 103 LEU . 53516 1 55 104 GLN . 53516 1 56 105 LEU . 53516 1 57 106 GLU . 53516 1 58 107 ALA . 53516 1 59 108 CYS . 53516 1 60 109 GLY . 53516 1 61 110 MET . 53516 1 62 111 ARG . 53516 1 63 112 ARG . 53516 1 64 113 LYS . 53516 1 65 114 SER . 53516 1 66 115 LEU . 53516 1 67 116 LEU . 53516 1 68 117 THR . 53516 1 69 118 ARG . 53516 1 70 119 LYS . 53516 1 71 120 VAL . 53516 1 72 121 ILE . 53516 1 73 122 CYS . 53516 1 74 123 LYS . 53516 1 75 124 SER . 53516 1 76 125 ASP . 53516 1 77 126 ALA . 53516 1 78 127 PRO . 53516 1 79 128 THR . 53516 1 80 129 GLY . 53516 1 81 130 ASP . 53516 1 82 131 VAL . 53516 1 83 132 LEU . 53516 1 84 133 LEU . 53516 1 85 134 ASP . 53516 1 86 135 GLU . 53516 1 87 136 ALA . 53516 1 88 137 LEU . 53516 1 89 138 LYS . 53516 1 90 139 HIS . 53516 1 91 140 VAL . 53516 1 92 141 LYS . 53516 1 93 142 GLU . 53516 1 94 143 THR . 53516 1 95 144 GLN . 53516 1 96 145 PRO . 53516 1 97 146 PRO . 53516 1 98 147 GLU . 53516 1 99 148 THR . 53516 1 100 149 VAL . 53516 1 101 150 GLN . 53516 1 102 151 ASN . 53516 1 103 152 TRP . 53516 1 104 153 ILE . 53516 1 105 154 GLU . 53516 1 106 155 LEU . 53516 1 107 156 LEU . 53516 1 108 157 SER . 53516 1 109 158 GLY . 53516 1 110 159 GLU . 53516 1 111 160 THR . 53516 1 112 161 TRP . 53516 1 113 162 ASN . 53516 1 114 163 PRO . 53516 1 115 164 LEU . 53516 1 116 165 LYS . 53516 1 117 166 LEU . 53516 1 118 167 HIS . 53516 1 119 168 TYR . 53516 1 120 169 GLN . 53516 1 121 170 LEU . 53516 1 122 171 ARG . 53516 1 123 172 ASN . 53516 1 124 173 VAL . 53516 1 125 174 ARG . 53516 1 126 175 GLU . 53516 1 127 176 ARG . 53516 1 128 177 LEU . 53516 1 129 178 ALA . 53516 1 130 179 LYS . 53516 1 131 180 ASN . 53516 1 132 181 LEU . 53516 1 133 182 VAL . 53516 1 134 183 GLU . 53516 1 135 184 LYS . 53516 1 136 185 GLY . 53516 1 137 186 VAL . 53516 1 138 187 LEU . 53516 1 139 188 THR . 53516 1 140 189 THR . 53516 1 141 190 GLU . 53516 1 142 191 LYS . 53516 1 143 192 GLN . 53516 1 144 193 ASN . 53516 1 145 194 PHE . 53516 1 146 195 GLY . 53516 1 147 196 GLY . 53516 1 148 197 GLY . 53516 1 149 198 ASP . 53516 1 150 199 MET . 53516 1 151 200 THR . 53516 1 152 201 THR . 53516 1 153 202 HIS . 53516 1 154 203 PRO . 53516 1 155 204 LEU . 53516 1 156 205 THR . 53516 1 157 206 ASN . 53516 1 158 207 ASN . 53516 1 159 208 ASN . 53516 1 160 209 ILE . 53516 1 161 210 LYS . 53516 1 162 211 GLN . 53516 1 163 212 ARG . 53516 1 164 213 LEU . 53516 1 165 214 ILE . 53516 1 166 215 LYS . 53516 1 167 216 LYS . 53516 1 168 217 VAL . 53516 1 169 218 GLN . 53516 1 170 219 GLU . 53516 1 171 220 ALA . 53516 1 172 221 VAL . 53516 1 173 222 LEU . 53516 1 174 223 ASP . 53516 1 175 224 LYS . 53516 1 176 225 TRP . 53516 1 177 226 VAL . 53516 1 178 227 ASN . 53516 1 179 228 ASP . 53516 1 180 229 PRO . 53516 1 181 230 HIS . 53516 1 182 231 ARG . 53516 1 183 232 MET . 53516 1 184 233 ASP . 53516 1 185 234 ARG . 53516 1 186 235 ARG . 53516 1 187 236 LEU . 53516 1 188 237 LEU . 53516 1 189 238 ALA . 53516 1 190 239 LEU . 53516 1 191 240 ILE . 53516 1 192 241 TYR . 53516 1 193 242 LEU . 53516 1 194 243 ALA . 53516 1 195 244 HIS . 53516 1 196 245 ALA . 53516 1 197 246 SER . 53516 1 198 247 ASP . 53516 1 199 248 VAL . 53516 1 200 249 LEU . 53516 1 201 250 GLU . 53516 1 202 251 ASN . 53516 1 203 252 ALA . 53516 1 204 253 PHE . 53516 1 205 254 ALA . 53516 1 206 255 PRO . 53516 1 207 256 LEU . 53516 1 208 257 LEU . 53516 1 209 258 ASP . 53516 1 210 259 GLU . 53516 1 211 260 GLN . 53516 1 212 261 TYR . 53516 1 213 262 ASP . 53516 1 214 263 LEU . 53516 1 215 264 ALA . 53516 1 216 265 THR . 53516 1 217 266 LYS . 53516 1 218 267 ARG . 53516 1 219 268 VAL . 53516 1 220 269 ARG . 53516 1 221 270 GLN . 53516 1 222 271 LEU . 53516 1 223 272 LEU . 53516 1 224 273 ASP . 53516 1 225 274 LEU . 53516 1 226 275 ASP . 53516 1 227 276 PRO . 53516 1 228 277 GLU . 53516 1 229 278 VAL . 53516 1 230 279 GLU . 53516 1 231 280 CYS . 53516 1 232 281 LEU . 53516 1 233 282 LYS . 53516 1 234 283 ALA . 53516 1 235 284 ASN . 53516 1 236 285 THR . 53516 1 237 286 ASN . 53516 1 238 287 GLU . 53516 1 239 288 VAL . 53516 1 240 289 LEU . 53516 1 241 290 TRP . 53516 1 242 291 ALA . 53516 1 243 292 VAL . 53516 1 244 293 VAL . 53516 1 245 294 ALA . 53516 1 246 295 ALA . 53516 1 247 296 PHE . 53516 1 248 297 THR . 53516 1 249 298 LYS . 53516 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 53516 1 . SER 2 2 53516 1 . LYS 3 3 53516 1 . GLY 4 4 53516 1 . ASP 5 5 53516 1 . SER 6 6 53516 1 . LYS 7 7 53516 1 . GLU 8 8 53516 1 . THR 9 9 53516 1 . ARG 10 10 53516 1 . LEU 11 11 53516 1 . THR 12 12 53516 1 . LEU 13 13 53516 1 . MET 14 14 53516 1 . GLU 15 15 53516 1 . GLU 16 16 53516 1 . VAL 17 17 53516 1 . LEU 18 18 53516 1 . LEU 19 19 53516 1 . LEU 20 20 53516 1 . GLY 21 21 53516 1 . LEU 22 22 53516 1 . LYS 23 23 53516 1 . ASP 24 24 53516 1 . ARG 25 25 53516 1 . GLU 26 26 53516 1 . GLY 27 27 53516 1 . TYR 28 28 53516 1 . THR 29 29 53516 1 . SER 30 30 53516 1 . PHE 31 31 53516 1 . TRP 32 32 53516 1 . ASN 33 33 53516 1 . ASP 34 34 53516 1 . CYS 35 35 53516 1 . ILE 36 36 53516 1 . SER 37 37 53516 1 . SER 38 38 53516 1 . GLY 39 39 53516 1 . LEU 40 40 53516 1 . ARG 41 41 53516 1 . GLY 42 42 53516 1 . CYS 43 43 53516 1 . MET 44 44 53516 1 . LEU 45 45 53516 1 . ILE 46 46 53516 1 . GLU 47 47 53516 1 . LEU 48 48 53516 1 . ALA 49 49 53516 1 . LEU 50 50 53516 1 . ARG 51 51 53516 1 . GLY 52 52 53516 1 . ARG 53 53 53516 1 . LEU 54 54 53516 1 . GLN 55 55 53516 1 . LEU 56 56 53516 1 . GLU 57 57 53516 1 . ALA 58 58 53516 1 . CYS 59 59 53516 1 . GLY 60 60 53516 1 . MET 61 61 53516 1 . ARG 62 62 53516 1 . ARG 63 63 53516 1 . LYS 64 64 53516 1 . SER 65 65 53516 1 . LEU 66 66 53516 1 . LEU 67 67 53516 1 . THR 68 68 53516 1 . ARG 69 69 53516 1 . LYS 70 70 53516 1 . VAL 71 71 53516 1 . ILE 72 72 53516 1 . CYS 73 73 53516 1 . LYS 74 74 53516 1 . SER 75 75 53516 1 . ASP 76 76 53516 1 . ALA 77 77 53516 1 . PRO 78 78 53516 1 . THR 79 79 53516 1 . GLY 80 80 53516 1 . ASP 81 81 53516 1 . VAL 82 82 53516 1 . LEU 83 83 53516 1 . LEU 84 84 53516 1 . ASP 85 85 53516 1 . GLU 86 86 53516 1 . ALA 87 87 53516 1 . LEU 88 88 53516 1 . LYS 89 89 53516 1 . HIS 90 90 53516 1 . VAL 91 91 53516 1 . LYS 92 92 53516 1 . GLU 93 93 53516 1 . THR 94 94 53516 1 . GLN 95 95 53516 1 . PRO 96 96 53516 1 . PRO 97 97 53516 1 . GLU 98 98 53516 1 . THR 99 99 53516 1 . VAL 100 100 53516 1 . GLN 101 101 53516 1 . ASN 102 102 53516 1 . TRP 103 103 53516 1 . ILE 104 104 53516 1 . GLU 105 105 53516 1 . LEU 106 106 53516 1 . LEU 107 107 53516 1 . SER 108 108 53516 1 . GLY 109 109 53516 1 . GLU 110 110 53516 1 . THR 111 111 53516 1 . TRP 112 112 53516 1 . ASN 113 113 53516 1 . PRO 114 114 53516 1 . LEU 115 115 53516 1 . LYS 116 116 53516 1 . LEU 117 117 53516 1 . HIS 118 118 53516 1 . TYR 119 119 53516 1 . GLN 120 120 53516 1 . LEU 121 121 53516 1 . ARG 122 122 53516 1 . ASN 123 123 53516 1 . VAL 124 124 53516 1 . ARG 125 125 53516 1 . GLU 126 126 53516 1 . ARG 127 127 53516 1 . LEU 128 128 53516 1 . ALA 129 129 53516 1 . LYS 130 130 53516 1 . ASN 131 131 53516 1 . LEU 132 132 53516 1 . VAL 133 133 53516 1 . GLU 134 134 53516 1 . LYS 135 135 53516 1 . GLY 136 136 53516 1 . VAL 137 137 53516 1 . LEU 138 138 53516 1 . THR 139 139 53516 1 . THR 140 140 53516 1 . GLU 141 141 53516 1 . LYS 142 142 53516 1 . GLN 143 143 53516 1 . ASN 144 144 53516 1 . PHE 145 145 53516 1 . GLY 146 146 53516 1 . GLY 147 147 53516 1 . GLY 148 148 53516 1 . ASP 149 149 53516 1 . MET 150 150 53516 1 . THR 151 151 53516 1 . THR 152 152 53516 1 . HIS 153 153 53516 1 . PRO 154 154 53516 1 . LEU 155 155 53516 1 . THR 156 156 53516 1 . ASN 157 157 53516 1 . ASN 158 158 53516 1 . ASN 159 159 53516 1 . ILE 160 160 53516 1 . LYS 161 161 53516 1 . GLN 162 162 53516 1 . ARG 163 163 53516 1 . LEU 164 164 53516 1 . ILE 165 165 53516 1 . LYS 166 166 53516 1 . LYS 167 167 53516 1 . VAL 168 168 53516 1 . GLN 169 169 53516 1 . GLU 170 170 53516 1 . ALA 171 171 53516 1 . VAL 172 172 53516 1 . LEU 173 173 53516 1 . ASP 174 174 53516 1 . LYS 175 175 53516 1 . TRP 176 176 53516 1 . VAL 177 177 53516 1 . ASN 178 178 53516 1 . ASP 179 179 53516 1 . PRO 180 180 53516 1 . HIS 181 181 53516 1 . ARG 182 182 53516 1 . MET 183 183 53516 1 . ASP 184 184 53516 1 . ARG 185 185 53516 1 . ARG 186 186 53516 1 . LEU 187 187 53516 1 . LEU 188 188 53516 1 . ALA 189 189 53516 1 . LEU 190 190 53516 1 . ILE 191 191 53516 1 . TYR 192 192 53516 1 . LEU 193 193 53516 1 . ALA 194 194 53516 1 . HIS 195 195 53516 1 . ALA 196 196 53516 1 . SER 197 197 53516 1 . ASP 198 198 53516 1 . VAL 199 199 53516 1 . LEU 200 200 53516 1 . GLU 201 201 53516 1 . ASN 202 202 53516 1 . ALA 203 203 53516 1 . PHE 204 204 53516 1 . ALA 205 205 53516 1 . PRO 206 206 53516 1 . LEU 207 207 53516 1 . LEU 208 208 53516 1 . ASP 209 209 53516 1 . GLU 210 210 53516 1 . GLN 211 211 53516 1 . TYR 212 212 53516 1 . ASP 213 213 53516 1 . LEU 214 214 53516 1 . ALA 215 215 53516 1 . THR 216 216 53516 1 . LYS 217 217 53516 1 . ARG 218 218 53516 1 . VAL 219 219 53516 1 . ARG 220 220 53516 1 . GLN 221 221 53516 1 . LEU 222 222 53516 1 . LEU 223 223 53516 1 . ASP 224 224 53516 1 . LEU 225 225 53516 1 . ASP 226 226 53516 1 . PRO 227 227 53516 1 . GLU 228 228 53516 1 . VAL 229 229 53516 1 . GLU 230 230 53516 1 . CYS 231 231 53516 1 . LEU 232 232 53516 1 . LYS 233 233 53516 1 . ALA 234 234 53516 1 . ASN 235 235 53516 1 . THR 236 236 53516 1 . ASN 237 237 53516 1 . GLU 238 238 53516 1 . VAL 239 239 53516 1 . LEU 240 240 53516 1 . TRP 241 241 53516 1 . ALA 242 242 53516 1 . VAL 243 243 53516 1 . VAL 244 244 53516 1 . ALA 245 245 53516 1 . ALA 246 246 53516 1 . PHE 247 247 53516 1 . THR 248 248 53516 1 . LYS 249 249 53516 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 53516 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 53516 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 53516 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET-Golph3 . . . 53516 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 53516 _Sample.ID 1 _Sample.Name '13C,15N GOlph3' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 Golph3 '[U-99% 13C; U-99% 15N]' . . 1 $entity_1 . . 300 . . uM . . . . 53516 1 2 H2O 'natural abundance' . . . . . . 90 . . % . . . . 53516 1 3 D2O 'natural abundance' . . . . . . 10 . . % . . . . 53516 1 4 MES 'natural abundance' . . . . . . 50 . . mM . . . . 53516 1 5 'sodium chloride' 'natural abundance' . . . . . . 225 . . mM . . . . 53516 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 53516 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Condition 1' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 225 . mM 53516 1 pH 6.5 0.1 pH 53516 1 pressure 1 . atm 53516 1 temperature 308 1 K 53516 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 53516 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 53516 1 processing . 53516 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 53516 _Software.ID 2 _Software.Type . _Software.Name CARA _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 53516 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 53516 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Bruker 800 at EPFL' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 53516 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 2 '3D HNCA' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 3 '3D HN(CO)CACB' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 4 '3D HNCO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 5 '3D HN(CA)CO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 6 '3D CBCACONH' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 7 '3D HNCACB' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53516 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 53516 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'Golph3 partial backbone assignment' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 DSS 'methyl protons' . . . . ppm 0 internal indirect . . . . . . 53516 1 H 1 DSS 'methyl protons' . . . . ppm 0 internal direct 1 . . . . . 53516 1 N 15 DSS 'methyl protons' . . . . ppm 0 internal indirect . . . . . . 53516 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 53516 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'Golph3 partial backbone assignment' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N HSQC' . . . 53516 1 2 '3D HNCA' . . . 53516 1 4 '3D HNCO' . . . 53516 1 5 '3D HN(CA)CO' . . . 53516 1 6 '3D CBCACONH' . . . 53516 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 2 $software_2 . . 53516 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 27 27 GLY H H 1 7.7302 . . . . . . . . 76 GLY H . 53516 1 2 . 1 . 1 27 27 GLY CA C 13 43.1271 . . . . . . . . 76 GLY CA . 53516 1 3 . 1 . 1 27 27 GLY N N 15 104.4004 . . . . . . . . 76 GLY N . 53516 1 4 . 1 . 1 28 28 TYR H H 1 7.3368 . . . . . . . . 77 TYR H . 53516 1 5 . 1 . 1 28 28 TYR CA C 13 52.6107 . . . . . . . . 77 TYR CA . 53516 1 6 . 1 . 1 28 28 TYR CB C 13 39.3925 . . . . . . . . 77 TYR CB . 53516 1 7 . 1 . 1 28 28 TYR N N 15 120.7746 . . . . . . . . 77 TYR N . 53516 1 8 . 1 . 1 146 146 GLY H H 1 8.2864 . . . . . . . . 195 GLY H . 53516 1 9 . 1 . 1 146 146 GLY CA C 13 42.6103 . . . . . . . . 195 GLY CA . 53516 1 10 . 1 . 1 146 146 GLY N N 15 108.7969 . . . . . . . . 195 GLY N . 53516 1 11 . 1 . 1 147 147 GLY H H 1 8.3448 . . . . . . . . 196 GLY H . 53516 1 12 . 1 . 1 147 147 GLY CA C 13 42.6175 . . . . . . . . 196 GLY CA . 53516 1 13 . 1 . 1 147 147 GLY N N 15 108.9055 . . . . . . . . 196 GLY N . 53516 1 14 . 1 . 1 148 148 GLY H H 1 8.3115 . . . . . . . . 197 GLY H . 53516 1 15 . 1 . 1 148 148 GLY C C 13 171.2667 . . . . . . . . 197 GLY C . 53516 1 16 . 1 . 1 148 148 GLY CA C 13 42.6223 . . . . . . . . 197 GLY CA . 53516 1 17 . 1 . 1 148 148 GLY N N 15 108.75 . . . . . . . . 197 GLY N . 53516 1 18 . 1 . 1 149 149 ASP H H 1 8.2825 . . . . . . . . 198 ASP H . 53516 1 19 . 1 . 1 149 149 ASP C C 13 173.8144 . . . . . . . . 198 ASP C . 53516 1 20 . 1 . 1 149 149 ASP CA C 13 51.655 . . . . . . . . 198 ASP CA . 53516 1 21 . 1 . 1 149 149 ASP CB C 13 38.5638 . . . . . . . . 198 ASP CB . 53516 1 22 . 1 . 1 149 149 ASP N N 15 120.2743 . . . . . . . . 198 ASP N . 53516 1 23 . 1 . 1 150 150 MET H H 1 8.4254 . . . . . . . . 199 MET H . 53516 1 24 . 1 . 1 150 150 MET C C 13 173.8309 . . . . . . . . 199 MET C . 53516 1 25 . 1 . 1 150 150 MET CA C 13 53.0886 . . . . . . . . 199 MET CA . 53516 1 26 . 1 . 1 150 150 MET CB C 13 29.9318 . . . . . . . . 199 MET CB . 53516 1 27 . 1 . 1 150 150 MET N N 15 120.4332 . . . . . . . . 199 MET N . 53516 1 28 . 1 . 1 151 151 THR H H 1 8.2657 . . . . . . . . 200 THR H . 53516 1 29 . 1 . 1 151 151 THR C C 13 171.9781 . . . . . . . . 200 THR C . 53516 1 30 . 1 . 1 151 151 THR CA C 13 59.7786 . . . . . . . . 200 THR CA . 53516 1 31 . 1 . 1 151 151 THR CB C 13 67.1529 . . . . . . . . 200 THR CB . 53516 1 32 . 1 . 1 151 151 THR N N 15 114.4234 . . . . . . . . 200 THR N . 53516 1 33 . 1 . 1 152 152 THR H H 1 8.0514 . . . . . . . . 201 THR H . 53516 1 34 . 1 . 1 152 152 THR CA C 13 58.8964 . . . . . . . . 201 THR CA . 53516 1 35 . 1 . 1 152 152 THR CB C 13 67.4606 . . . . . . . . 201 THR CB . 53516 1 36 . 1 . 1 152 152 THR N N 15 115.3135 . . . . . . . . 201 THR N . 53516 1 37 . 1 . 1 153 153 HIS H H 1 8.2315 . . . . . . . . 202 HIS H . 53516 1 38 . 1 . 1 153 153 HIS C C 13 170.2667 . . . . . . . . 202 HIS C . 53516 1 39 . 1 . 1 153 153 HIS CA C 13 51.5815 . . . . . . . . 202 HIS CA . 53516 1 40 . 1 . 1 153 153 HIS CB C 13 27.7911 . . . . . . . . 202 HIS CB . 53516 1 41 . 1 . 1 153 153 HIS N N 15 122.0959 . . . . . . . . 202 HIS N . 53516 1 42 . 1 . 1 196 196 ALA H H 1 7.5637 . . . . . . . . 245 ALA H . 53516 1 43 . 1 . 1 196 196 ALA C C 13 175.965 . . . . . . . . 245 ALA C . 53516 1 44 . 1 . 1 196 196 ALA CA C 13 51.9491 . . . . . . . . 245 ALA CA . 53516 1 45 . 1 . 1 196 196 ALA CB C 13 16.8112 . . . . . . . . 245 ALA CB . 53516 1 46 . 1 . 1 196 196 ALA N N 15 119.252 . . . . . . . . 245 ALA N . 53516 1 47 . 1 . 1 197 197 SER H H 1 7.0681 . . . . . . . . 246 SER H . 53516 1 48 . 1 . 1 197 197 SER C C 13 171.6638 . . . . . . . . 246 SER C . 53516 1 49 . 1 . 1 197 197 SER CA C 13 58.198 . . . . . . . . 246 SER CA . 53516 1 50 . 1 . 1 197 197 SER N N 15 102.7262 . . . . . . . . 246 SER N . 53516 1 51 . 1 . 1 198 198 ASP H H 1 7.6041 . . . . . . . . 247 ASP H . 53516 1 52 . 1 . 1 198 198 ASP CA C 13 51.7653 . . . . . . . . 247 ASP CA . 53516 1 53 . 1 . 1 198 198 ASP N N 15 123.2618 . . . . . . . . 247 ASP N . 53516 1 54 . 1 . 1 209 209 ASP H H 1 8.5439 . . . . . . . . 258 ASP H . 53516 1 55 . 1 . 1 209 209 ASP C C 13 174.9889 . . . . . . . . 258 ASP C . 53516 1 56 . 1 . 1 209 209 ASP CA C 13 55.6249 . . . . . . . . 258 ASP CA . 53516 1 57 . 1 . 1 209 209 ASP CB C 13 37.8208 . . . . . . . . 258 ASP CB . 53516 1 58 . 1 . 1 209 209 ASP N N 15 120.8278 . . . . . . . . 258 ASP N . 53516 1 59 . 1 . 1 210 210 GLU H H 1 9.1364 . . . . . . . . 259 GLU H . 53516 1 60 . 1 . 1 210 210 GLU C C 13 176.7921 . . . . . . . . 259 GLU C . 53516 1 61 . 1 . 1 210 210 GLU CA C 13 57.1687 . . . . . . . . 259 GLU CA . 53516 1 62 . 1 . 1 210 210 GLU CB C 13 26.2028 . . . . . . . . 259 GLU CB . 53516 1 63 . 1 . 1 210 210 GLU N N 15 118.5201 . . . . . . . . 259 GLU N . 53516 1 64 . 1 . 1 211 211 GLN H H 1 7.3913 . . . . . . . . 260 GLN H . 53516 1 65 . 1 . 1 211 211 GLN C C 13 174.1618 . . . . . . . . 260 GLN C . 53516 1 66 . 1 . 1 211 211 GLN CA C 13 56.1395 . . . . . . . . 260 GLN CA . 53516 1 67 . 1 . 1 211 211 GLN CB C 13 26.6791 . . . . . . . . 260 GLN CB . 53516 1 68 . 1 . 1 211 211 GLN N N 15 117.8191 . . . . . . . . 260 GLN N . 53516 1 69 . 1 . 1 212 212 TYR H H 1 8.7091 . . . . . . . . 261 TYR H . 53516 1 70 . 1 . 1 212 212 TYR CA C 13 59.9624 . . . . . . . . 261 TYR CA . 53516 1 71 . 1 . 1 212 212 TYR N N 15 121.7632 . . . . . . . . 261 TYR N . 53516 1 72 . 1 . 1 213 213 ASP H H 1 8.3054 . . . . . . . . 262 ASP H . 53516 1 73 . 1 . 1 213 213 ASP CA C 13 54.8162 . . . . . . . . 262 ASP CA . 53516 1 74 . 1 . 1 213 213 ASP N N 15 120.0985 . . . . . . . . 262 ASP N . 53516 1 75 . 1 . 1 214 214 LEU H H 1 7.2088 . . . . . . . . 263 LEU H . 53516 1 76 . 1 . 1 214 214 LEU CA C 13 54.8897 . . . . . . . . 263 LEU CA . 53516 1 77 . 1 . 1 214 214 LEU N N 15 120.1409 . . . . . . . . 263 LEU N . 53516 1 78 . 1 . 1 216 216 THR H H 1 7.6116 . . . . . . . . 265 THR H . 53516 1 79 . 1 . 1 216 216 THR CA C 13 63.1971 . . . . . . . . 265 THR CA . 53516 1 80 . 1 . 1 216 216 THR CB C 13 66.048 . . . . . . . . 265 THR CB . 53516 1 81 . 1 . 1 216 216 THR N N 15 107.8329 . . . . . . . . 265 THR N . 53516 1 82 . 1 . 1 217 217 LYS H H 1 7.7896 . . . . . . . . 266 LYS H . 53516 1 83 . 1 . 1 217 217 LYS C C 13 176.7756 . . . . . . . . 266 LYS C . 53516 1 84 . 1 . 1 217 217 LYS CA C 13 57.132 . . . . . . . . 266 LYS CA . 53516 1 85 . 1 . 1 217 217 LYS CB C 13 29.3794 . . . . . . . . 266 LYS CB . 53516 1 86 . 1 . 1 217 217 LYS N N 15 123.9872 . . . . . . . . 266 LYS N . 53516 1 87 . 1 . 1 244 244 VAL H H 1 7.949 . . . . . . . . 293 VAL H . 53516 1 88 . 1 . 1 244 244 VAL CA C 13 65.1085 . . . . . . . . 293 VAL CA . 53516 1 89 . 1 . 1 244 244 VAL N N 15 117.3537 . . . . . . . . 293 VAL N . 53516 1 90 . 1 . 1 245 245 ALA H H 1 8.5961 . . . . . . . . 294 ALA H . 53516 1 91 . 1 . 1 245 245 ALA C C 13 176.6763 . . . . . . . . 294 ALA C . 53516 1 92 . 1 . 1 245 245 ALA CA C 13 52.5004 . . . . . . . . 294 ALA CA . 53516 1 93 . 1 . 1 245 245 ALA CB C 13 14.8086 . . . . . . . . 294 ALA CB . 53516 1 94 . 1 . 1 245 245 ALA N N 15 122.1431 . . . . . . . . 294 ALA N . 53516 1 95 . 1 . 1 246 246 ALA H H 1 8.1231 . . . . . . . . 295 ALA H . 53516 1 96 . 1 . 1 246 246 ALA C C 13 176.1139 . . . . . . . . 295 ALA C . 53516 1 97 . 1 . 1 246 246 ALA CA C 13 51.655 . . . . . . . . 295 ALA CA . 53516 1 98 . 1 . 1 246 246 ALA CB C 13 14.6878 . . . . . . . . 295 ALA CB . 53516 1 99 . 1 . 1 246 246 ALA N N 15 120.1343 . . . . . . . . 295 ALA N . 53516 1 100 . 1 . 1 247 247 PHE H H 1 7.7406 . . . . . . . . 296 PHE H . 53516 1 101 . 1 . 1 247 247 PHE C C 13 173.9136 . . . . . . . . 296 PHE C . 53516 1 102 . 1 . 1 247 247 PHE CA C 13 56.0292 . . . . . . . . 296 PHE CA . 53516 1 103 . 1 . 1 247 247 PHE CB C 13 37.0001 . . . . . . . . 296 PHE CB . 53516 1 104 . 1 . 1 247 247 PHE N N 15 114.0837 . . . . . . . . 296 PHE N . 53516 1 105 . 1 . 1 248 248 THR H H 1 7.8556 . . . . . . . . 297 THR H . 53516 1 106 . 1 . 1 248 248 THR C C 13 171.1509 . . . . . . . . 297 THR C . 53516 1 107 . 1 . 1 248 248 THR CA C 13 60.5505 . . . . . . . . 297 THR CA . 53516 1 108 . 1 . 1 248 248 THR CB C 13 67.4982 . . . . . . . . 297 THR CB . 53516 1 109 . 1 . 1 248 248 THR N N 15 114.0999 . . . . . . . . 297 THR N . 53516 1 110 . 1 . 1 249 249 LYS H H 1 7.902 . . . . . . . . 298 LYS H . 53516 1 111 . 1 . 1 249 249 LYS C C 13 178.7442 . . . . . . . . 298 LYS C . 53516 1 112 . 1 . 1 249 249 LYS CA C 13 54.853 . . . . . . . . 298 LYS CA . 53516 1 113 . 1 . 1 249 249 LYS CB C 13 30.708 . . . . . . . . 298 LYS CB . 53516 1 114 . 1 . 1 249 249 LYS N N 15 129.6113 . . . . . . . . 298 LYS N . 53516 1 stop_ save_