BMRB Entry 53296
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53296
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NMR-STAR v3 text file.
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Citation: Toyama, Yuki; Inakami, Shinichiro; Takarada, Masaharu; Okabe, Kohki; Takeuchi, Koh; Shimada, Ichio. "Phase Separation Driven by Dynamic Interactions in the N-Terminal Intrinsically Disordered Region of the DEAD-Box RNA Helicase DDX3X" J. Am. Chem. Soc. 148, 21313-21331 (2026).
PubMed: 42159288
Assembly members:
entity_1, polymer, 132 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO
Entity Sequences (FASTA):
entity_1: MSHVAVENALGLDQQFAGLD
LNSSDNQSGGSTASKGRYIP
PHLRNREATKGFYDKDSSGW
SSSKDKDAYSSFGSRSDSRG
KSSFFSDRGSGSRGRFDDRG
RSDYDGIGSRGDRSGFGKFE
RGGNSRWCDKSD
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1
- heteronucl_T2_relaxation_2
- heteronucl_T2_relaxation_3
- heteronucl_T2_relaxation_4
- heteronucl_T2_relaxation_5
- heteronucl_T2_relaxation_6
- heteronucl_T2_relaxation_7
- heteronucl_T2_relaxation_8
- heteronucl_T2_relaxation_9
- heteronucl_T2_relaxation_10
- heteronucl_T2_relaxation_11
- heteronucl_T2_relaxation_12
- heteronucl_T2_relaxation_13
| Data type | Count |
| T1 relaxation values | 624 |
| T2 relaxation values | 1164 |
| heteronuclear NOE values | 354 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-IDR | 1 |
Entities:
Entity 1, N-IDR 132 residues - Formula weight is not available
| 1 | MET | SER | HIS | VAL | ALA | VAL | GLU | ASN | ALA | LEU | ||||
| 2 | GLY | LEU | ASP | GLN | GLN | PHE | ALA | GLY | LEU | ASP | ||||
| 3 | LEU | ASN | SER | SER | ASP | ASN | GLN | SER | GLY | GLY | ||||
| 4 | SER | THR | ALA | SER | LYS | GLY | ARG | TYR | ILE | PRO | ||||
| 5 | PRO | HIS | LEU | ARG | ASN | ARG | GLU | ALA | THR | LYS | ||||
| 6 | GLY | PHE | TYR | ASP | LYS | ASP | SER | SER | GLY | TRP | ||||
| 7 | SER | SER | SER | LYS | ASP | LYS | ASP | ALA | TYR | SER | ||||
| 8 | SER | PHE | GLY | SER | ARG | SER | ASP | SER | ARG | GLY | ||||
| 9 | LYS | SER | SER | PHE | PHE | SER | ASP | ARG | GLY | SER | ||||
| 10 | GLY | SER | ARG | GLY | ARG | PHE | ASP | ASP | ARG | GLY | ||||
| 11 | ARG | SER | ASP | TYR | ASP | GLY | ILE | GLY | SER | ARG | ||||
| 12 | GLY | ASP | ARG | SER | GLY | PHE | GLY | LYS | PHE | GLU | ||||
| 13 | ARG | GLY | GLY | ASN | SER | ARG | TRP | CYS | ASP | LYS | ||||
| 14 | SER | ASP |
Samples:
sample_1: potassium phosphate 20 mM; potassium chloride 200 mM; EDTA 1 mM; DTT 5 mM; N-IDR, [U-99% 15N], 300 uM
sample_2: potassium phosphate 20 mM; potassium chloride 200 mM; EDTA 1 mM; DTT 5 mM; N-IDR, [U-2H; U-15N], 100 uM
sample_3: potassium phosphate 20 mM; potassium chloride 200 mM; EDTA 1 mM; DTT 5 mM; N-IDR, [U-2H; U-15N], 300 uM
sample_4: potassium phosphate 20 mM; potassium chloride 200 mM; EDTA 1 mM; DTT 5 mM; N-IDR, [U-2H; U-15N], 500 uM
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 283.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| T1rho/R1rho relaxation | sample_1 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HxNx | sample_2 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HxNz | sample_2 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HzNx | sample_2 | isotropic | sample_conditions_1 |
| T1/R1 relaxation HzNz | sample_2 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation | sample_2 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_2 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HxNx | sample_3 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HxNz | sample_3 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HzNx | sample_3 | isotropic | sample_conditions_1 |
| T1/R1 relaxation HzNz | sample_3 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation | sample_3 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_3 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_3 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HxNx | sample_4 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HxNz | sample_4 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation HzNx | sample_4 | isotropic | sample_conditions_1 |
| T1/R1 relaxation HzNz | sample_4 | isotropic | sample_conditions_1 |
| T1rho/R1rho relaxation | sample_4 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_4 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_4 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1.4 - collection
NMRPipe v11.1 - processing
NMRFAM-SPARKY v1.470 - data analysis
peakipy v0.1.30 - data analysis
NMR spectrometers:
- Bruker AVANCE NEO 1000 MHz