BMRB Entry 53244

Name: 1H Chemical Shift Assignments of the HCV 5BSL3.2 apical stem loop element
Published: 2025-12-20

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PDB ID: 9w1l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53244
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H Chemical Shift Assignments of the HCV 5BSL3.2 apical stem loop element

Deposition date:

2025-06-25

Original release date:

2025-12-20

Authors:

kumar, Ajit; Penumutchu, Srinivas; Panchariya, Love; Daripa, Purba; Arulandu, Arockiasamy; Deshmukh, Mandar; Maiti, Souvik

Citation:

Citation: Kumar, Ajit; Penumutchu, Srinivas; Panchariya, Love; Kumari, Priyanka; Thakur, Shubham; Daripa, Purba; Singh, Vandana; Arulandu, Arockiasamy; Maiti, Souvik; Deshmukh, Mandar; Jain, Niyati. "Loop of Fate: Structural and Mechanistic Insights into hnRNPA1 Binding to the Hepatitis C Virus RNA" RNA ., .-. (2025).
PubMed: 41285614

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Hepatitis C Virus Taxonomy ID: 3052230 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus hominis

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAGACAUAUAUCACAGCCU GUCUCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	71
15N chemical shifts	7
1H chemical shifts	184

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	5BSL3.2 Apical stem loop	1

Entities:

Entity 1, 5BSL3.2 Apical stem loop 26 residues - Formula weight is not available

1

G

A

G

A

C

A

U

A

U

2

A

U

C

A

C

A

G

C

U

3

G

U

C

U

C

Samples:

sample_1: 5BSL3.2 Apical stem loop, [rRTPs (3',4',5',5''), and rYTPs (5,3',4',5',5'')], 200 uM

sample_conditions_1: ionic strength: 5 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
TOCSY	sample_1	isotropic	sample_conditions_1
HMQC	sample_1	isotropic	sample_conditions_1
HSQC	sample_1	isotropic	sample_conditions_1

Software:

topspin4, NMRPipe/nmrdraw,NMRViewJ, XplorNIH 2.25 - chemical shift assignment, collection, structure solution

NMR spectrometers:

Bruker Avance II 600 MHz